LOCUS       sequence-193636-        1350 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of N-terminal region of human HRASLS3 in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Han BG, Cho JW, Cho YD, Kim SY, Yoon HJ, Song HK, Cheong HK, Jeon 
            YH, Lee DK, Lee S, Lee BI
  TITLE     Expression, purification and biochemical characterization of the 
            N-terminal regions of human TIG3 and HRASLS3 proteins.
  JOURNAL   Protein Expr Purif. 2010 May;71(1):103-7. doi: 
            10.1016/j.pep.2010.01.018. Epub 2010 Jan 25.
  PUBMED    20100577
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.pep.2010.01.018"; journalName: "Protein Expr Purif"; 
            date: "2010-05"; volume: "71"; issue: "1"; pages: "103-7"
COMMENT     Sequence Label: pET28b-HRASLS3-1-132
FEATURES             Location/Qualifiers
     source          1..1350
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             33..55
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             74..91
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             101..118
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             527..544
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(597..615)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      611..658
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      695..1150
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(782..801)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     992..1013
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctatagaacg gtcaattccc tctagaaata attttgttta actttaagaa ggagatatac
       61 catgggcagc agccatcatc atcatcatca cagcagcggc ctggtgccgc gcggcagcca
      121 tatgcgtgcg cccattccag agcctaagcc tggagacctg attgagattt ttcgcccttt
      181 ctacagacac tgggccatct atgttggcga tggatatgtg gttcatctgg cccctccaag
      241 tgaggtcgca ggagctggtg cagccagtgt catgtccgcc ctgactgaca aggccatcgt
      301 gaagaaggaa ttgctgtatg atgtggccgg gagtgacaag taccaggtca acaacaaaca
      361 tgatgacaag tactcgccgc tgccctgcag caaaatcatc cagcgggcgg aggagctggt
      421 ggggcaggag gtgctctaca agctgaccag tgagaactgc gagcactttg tgaatgagct
      481 gcgctatgga gtcgcccgca gtgaccaggt cagagattaa ctcgagcacc accaccacca
      541 ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
      601 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
      661 gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc
      721 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
      781 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
      841 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
      901 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
      961 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     1021 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     1081 tggttaaaaa atgagctgat ttaccaaaaa tttaacgcga attttaacaa aatattaacg
     1141 cttacaattt aggtggcact tttcggggaa atgtgcccgg aacccctatt tgttattttt
     1201 caaaaacatt caaatatgtt ccggtcatga ataattctta gaaaaccctc cgagctccat
     1261 ggaactggca tttttctatc cggatttcaa tcccattttt ggaaaagcgg ttctgtatga
     1321 agagaaactc ccgaggcgtt ccaagggagg
//
