LOCUS       sequence-193638-        1271 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human transglutaminase 2 in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Han BG, Cho JW, Cho YD, Jeong KC, Kim SY, Lee BI
  TITLE     Crystal structure of human transglutaminase 2 in complex with 
            adenosine triphosphate.
  JOURNAL   Int J Biol Macromol. 2010 Aug 1;47(2):190-5. doi: 
            10.1016/j.ijbiomac.2010.04.023. Epub 2010 May 5.
  PUBMED    20450932
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijbiomac.2010.04.023"; journalName: "Int J Biol 
            Macromol"; date: "2010-08-1- 1"; volume: "47"; issue: "2"; pages: 
            "190-5"
COMMENT     Sequence Label: pHis-hTG2
FEATURES             Location/Qualifiers
     source          1..1271
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(51..74)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gtgaatcgct ctttcgggct ttgtttagca gcctaggtat taatcaatta gtggtggtgg
       61 tggtggtggt ggtgctcgag ggcggggcca atgatgacat tccggaagcc cttcacagcc
      121 ttcagcttgt cgctctcgaa gttcaccacc agcttgtgga ggcccatgtg gagcggcagc
      181 aggtccattc tcaccttaac ttcctcccct gcctccacgg ggtctgggat ctccaccgtc
      241 ttctgctcct cagtcaggcc ggccccctcc acagtgaagg tgcagccttc cagggccaca
      301 gggagcgggt tctgcaggga cacctcagcc accagcttgc gtttctgctt gggctcccca
      361 aggatccgga tcttgatttc tggattctcc aggtagaggt cacgctcagc cagcaggtag
      421 ctgttgataa ctggctccac gaggagggcc cgcaccttga tgaggttgga ctccgtaagg
      481 cagtcacggt atttctcata gaggatgcaa agaggaacgc tcttctcaga gaaaggctcc
      541 aggttgaggt tgagcaggta cttggtgcca cactcgggcc ccaagatccc attgtagctg
      601 acggtgcggg cacagagcag gaggcggcag acgtactcct cagcggtgtt gttggtgatg
      661 tgggcaaaga cgtcaaagtc actgcccatg ttcatgctct ggcccacacg gatccgcatg
      721 gccatccctg tctcctcctt ctcggccagt ttgttcaggt ggttcgccct tgtgaaggcc
      781 tccctctcct ctgaggaccc ctctgggtat ttgtaggtgt gggtgatatc ctcccgctcg
      841 tctcggccca cgctcttagt gctgatcttc agcccaacga tcagggaacg gttgatggat
      901 ttgtgcacag acccatcgtc ctgctggatc cagtctacca cgtcggcatt gacctccgca
      961 aagacaaagg gcgcatcgta cttggtgctc aggtcgccct ccttgatggc acgaactgga
     1021 actgggccac agcagtacgt cccttcgctc ttctcctggg gcgttgggtc cagggcctgc
     1081 cagccctcgt accccggctg caggtccggc ctggtcatcc acgactccac ccagcatggg
     1141 aagttcccga tcatctccct cttggcaccc tggatttccc caaactcatt gcggaaataa
     1201 tccgatgaaa aagtttgttg tttttggtca tgggcccaaa tttgaatttg gtcacaaatt
     1261 tcttttttgt t
//
