LOCUS       sequence-193662-        9180 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gRNA with 16 MCP binding site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9180)
  AUTHORS   Qin P, Parlak M, Kuscu C, Bandaria J, Mir M, Szlachta K, Singh R, 
            Darzacq X, Yildiz A, Adli M
  TITLE     Live cell imaging of low- and non-repetitive chromosome loci using 
            CRISPR-Cas9.
  JOURNAL   Nat Commun. 2017 Mar 14;8:14725. doi: 10.1038/ncomms14725.
  PUBMED    28290446
REFERENCE   2  (bases 1 to 9180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-03-14"; volume: "8:14725. doi"; pages: " 
            10.1038/ncomms14725"
COMMENT     Sequence Label: sg2.02 16x(MS2) MUC4.1
FEATURES             Location/Qualifiers
     source          1..9180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(195..228)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     232..252
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_RNA        359..377
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        434..452
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        512..530
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        551..569
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        621..639
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        660..678
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        730..748
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        769..787
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        839..857
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        878..896
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        948..966
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        987..1005
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1057..1075
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1096..1114
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1166..1184
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1205..1223
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     enhancer        1427..1730
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1731..1934
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             1968..2564
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1968..1987)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2464..2484
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             2574..2627
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2640..3341
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     protein_bind    complement(3361..3394)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3450..4038
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3503..3523)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3921..3932)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4041..4057)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4042..4058)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4567..4747
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(4809..5397)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4889..4908)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5568..6359,6360..6428))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6191..6210
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6429..6533)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6608..6627)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6799..7178
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7180..7378
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7396..7576
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7623..7748
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8245..8478
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8663..8707
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9005..9122
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gatccgacgc gccatctcta ggcccgcgcc ggccccctcg cacggacttg tgggagaagc
       61 tcggctactc ccctgccccg gttaatttgc atataatatt tcctagtaac tatagaggct
      121 taatgtgcga taaaagacag ataatctgtt ctttttaata ctagctacat tttacatgat
      181 aggcttggat ttctataact tcgtatagca tacattatac gaagttataa acagcacaaa
      241 aggaaactca ccctaactgt aaagtaattg tgtgttttga gactataagt atcccttgga
      301 gaaccacctt gttgggtaaa gtagaaaagg cataaagttt aagagctatg ctgggccaac
      361 atgaggatca cccatgtctg cagggcccag catagcaagt ttaaataagg ctagtccgtt
      421 atcaacttgg ccaacatgag gatcacccat gtctgcaggg ccaagtggca ccgagtcggt
      481 gcggatccta aggtacctaa ttgcctagaa aacatgagga tcacccatgt ctgcaggtcg
      541 actctagaaa acatgaggat cacccatgtc tgcagtattc ccgggttcat tagatcctaa
      601 ggtacctaat tgcctagaaa acatgaggat cacccatgtc tgcaggtcga ctctagaaaa
      661 catgaggatc acccatgtct gcagtattcc cgggttcatt agatcctaag gtacctaatt
      721 gcctagaaaa catgaggatc acccatgtct gcaggtcgac tccagaaaac atgaggatca
      781 cccatgtctg cagtattccc gggttcatta gatcctaagg tacctaattg cctagaaaac
      841 atgaggatca cccatgtctg caggtcgact ccagaaaaca tgaggatcac ccatgtctgc
      901 agtattcccg ggttcattag atcctaaggt acctaattgc ctagaaaaca tgaggatcac
      961 ccatgtctgc aggtcgactc tagaaaacat gaggatcacc catgtctgca gtattcccgg
     1021 gttcattaga tcctaaggta cctaattgcc tagaaaacat gaggatcacc catgtctgca
     1081 ggtcgactct agaaaacatg aggatcaccc atgtctgcag tattcccggg ttcattagat
     1141 cctaaggtac ctaattgcct agaaaacatg aggatcaccc atgtctgcag gtcgactcca
     1201 gaaaacatga ggatcaccca tgtctgcagt attcccgggt tcattagatc tgcgcgcaat
     1261 agcaagttaa aataatatta gtccgttttt agcgcgtgcg ccaattctgc aggggcgcct
     1321 ttttttatta ggcggccgcg tggataaccg tattaccgcc atgcattagt tattaatagt
     1381 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     1441 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     1501 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     1561 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     1621 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     1681 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     1741 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     1801 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     1861 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     1921 atataagcag agctggttta gtgaaccgtc agatccgcta gcccaccatg accgagtaca
     1981 agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg
     2041 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc
     2101 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     2161 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     2221 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     2281 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     2341 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     2401 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     2461 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     2521 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgccggatcg ggagagggca
     2581 gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc tggcccaccg gtcgccacca
     2641 tgagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca
     2701 accatcactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca
     2761 tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta
     2821 gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca
     2881 agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg
     2941 tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga
     3001 tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg
     3061 aggccttcac cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg
     3121 ccctgaagct cgtgggcggg agccatctga tcgcaaacat caagaccaca tatagatcca
     3181 agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa
     3241 gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat
     3301 actgcgacct ccctagcaaa ctggggcaca agcttaatta agaattcgtc gagggaccta
     3361 ataacttcgt atagcataca ttatacgaag ttatacatgt ttaagggttc cggttccact
     3421 aggtacaatt cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     3481 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     3541 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     3601 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     3661 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     3721 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     3781 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     3841 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     3901 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     3961 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     4021 tttgggccgc ctccccgcat cgataccgtc gacctcgatc gagacctaga aaaacatgga
     4081 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     4141 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     4201 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     4261 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     4321 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg
     4381 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac
     4441 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta
     4501 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac
     4561 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     4621 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     4681 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     4741 tctagcagca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     4801 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     4861 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     4921 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     4981 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     5041 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     5101 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     5161 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     5221 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     5281 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     5341 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     5401 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     5461 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     5521 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     5581 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     5641 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     5701 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     5761 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     5821 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     5881 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     5941 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     6001 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     6061 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     6121 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     6181 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     6241 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     6301 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     6361 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     6421 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     6481 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     6541 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
     6601 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     6661 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     6721 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
     6781 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     6841 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     6901 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     6961 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     7021 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     7081 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     7141 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     7201 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     7261 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     7321 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg
     7381 cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     7441 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7501 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7561 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     7621 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     7681 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     7741 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     7801 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     7861 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     7921 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     7981 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     8041 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat
     8101 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     8161 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     8221 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     8281 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     8341 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     8401 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     8461 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     8521 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     8581 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     8641 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     8701 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     8761 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     8821 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     8881 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     8941 attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc
     9001 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     9061 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     9121 ttcgggttta ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga
//
