LOCUS       sequence-193664-        9120 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gRNA with 14 MCP binding site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9120)
  AUTHORS   Qin P, Parlak M, Kuscu C, Bandaria J, Mir M, Szlachta K, Singh R, 
            Darzacq X, Yildiz A, Adli M
  TITLE     Live cell imaging of low- and non-repetitive chromosome loci using 
            CRISPR-Cas9.
  JOURNAL   Nat Commun. 2017 Mar 14;8:14725. doi: 10.1038/ncomms14725.
  PUBMED    28290446
REFERENCE   2  (bases 1 to 9120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-03-14"; volume: "8:14725. doi"; pages: " 
            10.1038/ncomms14725"
COMMENT     Sequence Label: sg14x(MS2) MUC4.1
FEATURES             Location/Qualifiers
     source          1..9120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(195..228)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     232..252
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_RNA        452..470
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        491..509
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        561..579
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        600..618
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        670..688
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        709..727
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        779..797
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        818..836
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        888..906
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        927..945
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        997..1015
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1036..1054
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1106..1124
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1145..1163
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     enhancer        1367..1670
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1671..1874
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             1908..2504
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1908..1927)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2404..2424
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             2514..2567
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2580..3281
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     protein_bind    complement(3301..3334)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3390..3978
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3443..3463)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3861..3872)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3981..3997)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3982..3998)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4507..4687
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(4749..5337)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4829..4848)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5508..6299,6300..6368))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6131..6150
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6369..6473)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6548..6567)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6739..7118
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7120..7318
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7336..7516
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7563..7688
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8185..8418
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8603..8647
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8945..9062
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gatccgacgc gccatctcta ggcccgcgcc ggccccctcg cacggacttg tgggagaagc
       61 tcggctactc ccctgccccg gttaatttgc atataatatt tcctagtaac tatagaggct
      121 taatgtgcga taaaagacag ataatctgtt ctttttaata ctagctacat tttacatgat
      181 aggcttggat ttctataact tcgtatagca tacattatac gaagttataa acagcacaaa
      241 aggaaactca ccctaactgt aaagtaattg tgtgttttga gactataagt atcccttgga
      301 gaaccacctt gttgggtaaa gtagaaaagg cataaagttt aagagctatg ctggaaacag
      361 catagcaagt ttaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
      421 gcggatccta aggtacctaa ttgcctagaa aacatgagga tcacccatgt ctgcaggtcg
      481 actctagaaa acatgaggat cacccatgtc tgcagtattc ccgggttcat tagatcctaa
      541 ggtacctaat tgcctagaaa acatgaggat cacccatgtc tgcaggtcga ctctagaaaa
      601 catgaggatc acccatgtct gcagtattcc cgggttcatt agatcctaag gtacctaatt
      661 gcctagaaaa catgaggatc acccatgtct gcaggtcgac tccagaaaac atgaggatca
      721 cccatgtctg cagtattccc gggttcatta gatcctaagg tacctaattg cctagaaaac
      781 atgaggatca cccatgtctg caggtcgact ccagaaaaca tgaggatcac ccatgtctgc
      841 agtattcccg ggttcattag atcctaaggt acctaattgc ctagaaaaca tgaggatcac
      901 ccatgtctgc aggtcgactc tagaaaacat gaggatcacc catgtctgca gtattcccgg
      961 gttcattaga tcctaaggta cctaattgcc tagaaaacat gaggatcacc catgtctgca
     1021 ggtcgactct agaaaacatg aggatcaccc atgtctgcag tattcccggg ttcattagat
     1081 cctaaggtac ctaattgcct agaaaacatg aggatcaccc atgtctgcag gtcgactcca
     1141 gaaaacatga ggatcaccca tgtctgcagt attcccgggt tcattagatc tgcgcgcaat
     1201 agcaagttaa aataatatta gtccgttttt agcgcgtgcg ccaattctgc aggggcgcct
     1261 ttttttatta ggcggccgcg tggataaccg tattaccgcc atgcattagt tattaatagt
     1321 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     1381 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     1441 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     1501 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     1561 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     1621 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     1681 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     1741 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     1801 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     1861 atataagcag agctggttta gtgaaccgtc agatccgcta gcccaccatg accgagtaca
     1921 agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg
     1981 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc
     2041 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     2101 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     2161 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     2221 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     2281 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     2341 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     2401 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     2461 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgccggatcg ggagagggca
     2521 gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc tggcccaccg gtcgccacca
     2581 tgagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca
     2641 accatcactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca
     2701 tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta
     2761 gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca
     2821 agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg
     2881 tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga
     2941 tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg
     3001 aggccttcac cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg
     3061 ccctgaagct cgtgggcggg agccatctga tcgcaaacat caagaccaca tatagatcca
     3121 agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa
     3181 gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat
     3241 actgcgacct ccctagcaaa ctggggcaca agcttaatta agaattcgtc gagggaccta
     3301 ataacttcgt atagcataca ttatacgaag ttatacatgt ttaagggttc cggttccact
     3361 aggtacaatt cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     3421 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     3481 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     3541 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     3601 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     3661 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     3721 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     3781 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     3841 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     3901 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     3961 tttgggccgc ctccccgcat cgataccgtc gacctcgatc gagacctaga aaaacatgga
     4021 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     4081 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     4141 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     4201 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     4261 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg
     4321 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac
     4381 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta
     4441 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac
     4501 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     4561 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     4621 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     4681 tctagcagca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     4741 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     4801 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     4861 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     4921 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     4981 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     5041 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     5101 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     5161 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     5221 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     5281 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     5341 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     5401 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     5461 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     5521 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     5581 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     5641 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     5701 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     5761 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     5821 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     5881 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     5941 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     6001 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     6061 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     6121 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     6181 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     6241 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     6301 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     6361 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     6421 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     6481 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
     6541 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     6601 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     6661 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
     6721 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     6781 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     6841 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     6901 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     6961 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     7021 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     7081 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     7141 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     7201 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     7261 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg
     7321 cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     7381 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7441 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7501 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     7561 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     7621 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     7681 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     7741 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     7801 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     7861 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     7921 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     7981 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat
     8041 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     8101 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     8161 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     8221 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     8281 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     8341 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     8401 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     8461 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     8521 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     8581 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     8641 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     8701 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     8761 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     8821 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     8881 attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc
     8941 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     9001 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     9061 ttcgggttta ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga
//
