LOCUS       sequence-193692-        6088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV expression of FLEXed ArcLightD driven by hSynap promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6088)
  TITLE     Pieribone Lab AAV Arclight plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pENN.AAV.hSynap.Flex.ArcLightDco.WPRE.SV40
FEATURES             Location/Qualifiers
     source          1..6088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   33..162
                     /label=AAV2 ITR
     promoter        244..691
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    721..754
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    817..850
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      2359..2368
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2379..2412)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(2483..2516)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    2558..3146
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2611..2631)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3029..3040)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3149..3165)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3150..3166)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    3195..3316
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3206..3225)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3260..3279
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3478..3607
                     /label=AAV2 ITR
     rep_origin      complement(3844..4432)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3924..3943)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4603..5394,5395..5463))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5226..5245
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5464..5568)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5594..6049)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5731..5752)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5943..5962
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gatcggtgcg ggcctcttcg ctattacgcc agctgcgcgc tcgctcgctc actgaggccg
       61 cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag
      121 cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta atgattaacc
      181 cgccatgcta cttatctacg tagccatgct ctaggaagat ctctgcagag ggccctgcgt
      241 atgagtgcaa gtgggtttta ggaccaggat gaggcggggt gggggtgcct acctgacgac
      301 cgaccccgac ccactggaca agcacccaac ccccattccc caaattgcgc atcccctatc
      361 agagaggggg aggggaaaca ggatgcggcg aggcgcgtgc gcactgccag cttcagcacc
      421 gcggacagtg ccttcgcccc cgcctggcgg cgcgcgccac cgccgcctca gcactgaagg
      481 cgcgctgacg tcactcgccg gtcccccgca aactcccctt cccggccacc ttggtcgcgt
      541 ccgcgccgcc gccggcccag ccggaccgca ccacgcgagg cgcgagatag gggggcacgg
      601 gcgcgaccat ctgcgctgcg gcgccggcga ctcagcgctg cctcagtctg cggtgggcag
      661 cggaggagtc gtgtcgtgcc tgagagcgca gtcgaattca agctgctagc aaggatcccc
      721 ataacttcgt ataaagtatc ctatacgaag ttatatcaaa ataggaagac caatgcttca
      781 ccatcgaccc gaattgccaa gcatcaccat cgacccataa cttcgtataa tgtatgctat
      841 acgaagttat actactagta aggtacccag agctcactgt acattgtcta gggccgcttc
      901 acacctcgtt ctcgtagcag aacttataca gctcgtccat tccgtgggta atcccgtcgg
      961 cagtgacaaa ctccagcagc accatatgat ctcttttctc gttggggtcc ttagacagag
     1021 tggatgtggt gaacagatag tgattatctg ggagcaggac aggcccgtct ccgattggtg
     1081 tgttctgctg gtaatgatcg gccagctgca ctccgccgtc ttcaatgttg tggcggatct
     1141 tgaagttagc cttaatgccg tttttctgct tatcggccat gatgtacacc tgatggtcgt
     1201 tatagttgta ctcgagcttg tgccccagga tatttccgtc ttctttgaag tcaattccct
     1261 tgagctcgat tcggttgacc agggtgtcgc cttcaaactt cacctctgcg cgagttttat
     1321 aattgccgtc gtccttaaag aagatggtcc gttcctgcac gtagccctct ggcatagcag
     1381 atttaaagaa atcgtgtcgc ttcatatggt caggatagcg actgaaacac tgcacgccgt
     1441 atgtgagagt tgtgaccagg gtaggccaag gcactggcag cttcccggta gtgcagatga
     1501 atttcagggt gagctttccg taagtagcgt cgccttcacc ctccccgctc actgaaaact
     1561 tatggccgtt cacgtcgccg tcgagctcca ccagaatggg gaccacacca gtgaacagtt
     1621 cctccccttt agacattgga tccccctgct ggtgggagta aaagatcctt gccagtcgga
     1681 ccactcggag cagtcgagcg aggaccacca gctgtccgag tccgtctgct cctgtctcct
     1741 gcacgtactc gtccagcact gtgtagaaaa tggtgaccac aaaagtgacc acaatgatca
     1801 gtccgtcggc cacctcccaa ggattggtaa agaagttctt gggtccgtat gcgaagatcc
     1861 tcaggccgag atccagcatg aagtagcagc tgagggccag tgccatgccg tcgtagaagc
     1921 tctgggagct ctctgacttc ccagggagac tcagatcaat gatcatcaga atgatgtcca
     1981 ggaagatgag aaagacgccg aacacccgca ttcccagatg gtcgatgaca gctctcaccc
     2041 taaactggac tctacccact cctgtggtag gaatttccat cctcccgtcg tccacgttga
     2101 tgtcgatctg gtccaggggg atgtactcgc cctcgctggt gatcctgctc ttctcctgtc
     2161 ttgtttcggg cttctcgctt ggtccctgat ccacgtcgtt ccagtggact ttcttcacac
     2221 tctcagattt tctaggagct gccttaggtg gtgcagtcac gtcgccgttg attgtcacgt
     2281 ccaccacgtt ccgcatcaca gcaccgtcca cacccacgag atcggcagga gggctaaagt
     2341 ctgacccgtc aaaaccttcc atggtggcac cggctagtat aacttcgtat aggatacttt
     2401 atacgaagtt atcattggga ttcttcctat tttgatccaa gcatcaccat cgaccctcta
     2461 gtccagatct caccatcgac ccataacttc gtatagcata cattatacga agttatgtcc
     2521 ctcgaagagg ttcgaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     2581 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     2641 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     2701 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     2761 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     2821 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     2881 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     2941 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     3001 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     3061 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     3121 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgacccg ggcggccgct
     3181 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     3241 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     3301 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     3361 gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga
     3421 tcttcctaga gcatggctac gtagataagt agcatggcgg gttaatcatt aactacaagg
     3481 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     3541 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     3601 cgcgcagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     3661 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3721 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3781 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3841 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3901 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3961 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4021 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4081 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4141 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4201 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4261 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     4321 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4381 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4441 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4501 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4561 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     4621 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     4681 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     4741 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     4801 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     4861 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     4921 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     4981 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5041 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5101 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5161 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     5221 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     5281 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     5341 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     5401 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     5461 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     5521 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     5581 aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     5641 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     5701 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     5761 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     5821 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     5881 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     5941 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     6001 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca
     6061 ttcaggctgc gcaactgttg ggaagggc
//
