LOCUS       sequence-193705-        5913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express fusion protein of EnvA (ASLV-A envelope protein) 
            extracellular and transmembrane domains with rabies virus SAD B19 
            strain glycoprotein intracellular domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5913)
  TITLE     Genetic Neuroengineering Group Plasmids 2017
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAG-EnvARGCD
FEATURES             Location/Qualifiers
     source          1..5913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     336..358
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     420..439
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      470..479
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2397..2416)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2462..2517
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2516..2535)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2878..2894)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2878..2894)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2891..2913)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2902..2918
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2926..2932,2933..2950,2951..2956))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2971..2992
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3051..3246
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3097..3232
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3252..3386
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3289..3308)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3343..3362
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3454..3471)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3625..4213)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3705..3724)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4384..5175,5176..5244))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5007..5026
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5245..5349)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5380..5759
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg
       61 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
      121 acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc
      181 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct
      241 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg
      301 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
      361 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
      421 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattcgccg ccaccatgga
      481 agccgtcata aaggcatttc tgactgggca ccctggaaag gtgagcaaga aggactccaa
      541 gaagaagccg ccagcaacaa gcaagaaaga cccggagaag acgcccttgc tgccaacgag
      601 agttaattat attattggtg tcctggtctt gtgtgaggta acgggggtaa gagctgatgt
      661 ccacttactc gagcagccag ggaacctttg gattacatgg gccaaccgta caggccaaac
      721 ggatttttgc ctctctacac agtcagccac ctcccctttt caaacatgtt tgataggtat
      781 cccgtcccct atttccgagg gtgattttaa gggatatgtt tctgatacaa attgcaccac
      841 cttgggaact gatcggttag tctcgtcagc cgactttact ggcggacctg acaacagtac
      901 caccctcact tatcggaagg tctcatgctt gttgttaaag ctgaatgtct ctatgtggga
      961 tgagccacct gaactacagc tgttaggttc ccagtctctc cctaacatta ctaatattgc
     1021 tcagatttcc ggtataaccg ggggatgcgt aggcttcaga ccacaagggg ttccttggta
     1081 tctaggttgg tctagacagg aggccacgcg gtttctcctt agacacccct ctttctctaa
     1141 atccacggaa ccgtttacgg tggtgacagc ggataggcac aatcttttta tggggagtga
     1201 gtactgcggt gcatatggct acagattttg gaacatgtat aactgctcac aggtggggcg
     1261 gcagtaccgc tgtggtaatg cgcgcagccc ccgcccgggt cttcctgaaa tccagtgtac
     1321 aaggagagga ggcaaatggg ttaatcaatc acaggaaatt aatgagtcgg agccgttcag
     1381 ctttacggtg aactgtacag ctagtagttt gggtaatgcc agtgggtgtt gcggaaaagc
     1441 aggcacgatt ctcccgggaa agtgggtcga cagcacacaa ggtagtttca ccaaaccaaa
     1501 agcgctacca cccgcaattt tcctcatttg tggggatcgc gcatggcaag gaattcccag
     1561 tcgtccggta gggggcccct gctatttagg caagcttacc atgttagcac ctaagcatac
     1621 agatattctc aaggtgcttg tcaattcatc gcggacaggt ataagacgta aacgaagcac
     1681 ctcacacctg gatgatacat gctcagatga agtgcagctt tggggtccta cagcaagaat
     1741 ctttgcatct atcctagccc cgggggtagc agctgcgcaa gccttaagag aaattgagag
     1801 actagcctgt tggtccgtta aacaggctaa cttgacaaca tcactcctcg gggacttatt
     1861 ggatgatgtc acgagtattc gacacgcggt cctgcagaac cgagcggcta ttgacttctt
     1921 gctcctagct cacggccatg gctgtgagga cgttgccgga atgtgctgtt tcaatttgag
     1981 tgatcacagt gagtctatac agaagaagtt ccagctaatg aaggaacatg tcaataagat
     2041 cggcgtggac agcgacccaa ttggaagttg gctgcgagga ctattcgggg gaataggaga
     2101 atgggccgtt catttgctga aaggactgct tttggggctt gtagttattt tgttgctagt
     2161 agtgtgcctg ccttgcagaa gagtcaatcg atcagaacct acgcaacaca atctcagagg
     2221 gacagggagg gaggtgtcag tcactcccca aagcgggaag atcatatctt catgggaatc
     2281 acacaagagt gggggtgaga ccagactgtg agcggccgca ctcctcaggt gcaggctgcc
     2341 tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt
     2401 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
     2461 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
     2521 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
     2581 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat
     2641 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
     2701 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
     2761 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
     2821 gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc
     2881 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     2941 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     3001 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat
     3061 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     3121 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     3181 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     3241 tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     3301 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     3361 tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag
     3421 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     3481 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3541 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3601 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3661 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3721 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3781 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     3841 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3901 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3961 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     4021 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     4081 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4141 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4201 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     4261 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     4321 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     4381 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     4441 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     4501 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     4561 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     4621 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     4681 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     4741 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     4801 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     4861 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     4921 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     4981 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     5041 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     5101 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     5161 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     5221 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     5281 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     5341 ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg acattgatta ttgactagtt
     5401 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     5461 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     5521 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     5581 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     5641 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     5701 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     5761 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
     5821 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
     5881 cgcgcgccag gcggggcggg gcggggcgag ggg
//
