LOCUS       sequence-193708-        5503 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express rabies virus CVS N2c strain nucleoprotein gene (N2cN).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5503)
  TITLE     Genetic Neuroengineering Group Plasmids 2017
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAG-N2cN
FEATURES             Location/Qualifiers
     source          1..5503
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     402..424
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     486..505
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      536..545
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1980..1999)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2045..2100
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2099..2118)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2461..2477)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2461..2477)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2474..2496)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2485..2501
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2509..2515,2516..2533,2534..2539))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2554..2575
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2634..2829
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2680..2815
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2835..2969
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2872..2891)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2926..2945
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3037..3054)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3208..3796)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3288..3307)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3967..4758,4759..4827))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4590..4609
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4828..4932)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4963..5342
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
       61 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
      121 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
      181 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
      241 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
      301 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
      361 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
      421 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
      481 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgccgccac
      541 catggatgcc gacaagattg tgttcaaagt caataatcag gtggtctctt tgaagcctga
      601 gattatcgtg gatcaatatg agtacaagta ccctgccatc aaggatttga aaaagccttg
      661 tatcacccta gggaaagccc ccgacttgaa caaagcatac aaatcagttt tatcaggcat
      721 gaatgccgcc aaacttgatc cggatgatgt atgctcctac ttggcagcag caatgcagtt
      781 ctttgagggg acatgtccgg aagactggac cagctatgga atcctgattg cacgaaaagg
      841 agataggatc accccaaact ctctagtgga gataaagcgt actgatgtag aagggaattg
      901 ggctctgaca ggaggcatgg aattgacaag ggaccccact gtctctgaac atgcatcttt
      961 agtcggtctt ctcctgagtc tgtacaggtt gagcaaaata tcaggacaga acactggtaa
     1021 ctataagaca aacattgcag ataggataga gcagattttc gagacagcac cttttgttaa
     1081 gatcgtggaa caccataccc taatgacaac tcacaagatg tgtgctaatt ggagtactat
     1141 accgaacttc agatttttgg ccggaaccta cgacatgttt ttctcacgga ttgagcatct
     1201 atattcggca atcagagtgg gcacagtcgt caccgcttat gaagactgct caggactggt
     1261 atcgtttaca gggttcataa agcagatcaa tctcaccgca agggaagcaa tactatattt
     1321 cttccacaag aactttgagg aagagataag aagaatgttc gagccagggc aagagacagc
     1381 tgttcctcac tcttatttca tccacttccg ttcactaggc ttgagtggga agtctcctta
     1441 ttcatcgaat gctgtcggtc atgtgttcaa tctcattcac tttgttggat gctacatggg
     1501 tcaagtcaga tctctaaatg cgacggttat tgctgcatgt gcccctcatg agatgtctgt
     1561 tctagggggc tatttgggag aggaattctt cggaaaaggg acatttgaaa gaaggttctt
     1621 cagagacgag aaagaacttc aagaatatga ggcggctgaa ctaacaaagt ccgacgtggc
     1681 actggcagat gacggaaccg tcaactctga tgacgaggac tatttctctg gtgaaaccag
     1741 aagtccagaa gctgtctata ctcgaatcat gatgaatgga ggtcgactga agagatctca
     1801 tatacggaga tatgtctcag tcagttccaa tcatcaagcc cgtccaaact cattcgccga
     1861 atttttaaac aagacgtatt cgaatgactc ataagcggcc gcactcctca ggtgcaggct
     1921 gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat
     1981 ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct
     2041 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca
     2101 ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag
     2161 agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt
     2221 catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact
     2281 tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa
     2341 ttttccttac atgttttact agccagattt ttcctcctct cctgactact cccagtcata
     2401 gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg
     2461 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     2521 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     2581 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgcatctc
     2641 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     2701 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     2761 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     2821 ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     2881 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     2941 tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg
     3001 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     3061 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     3121 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     3181 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3241 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3301 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     3361 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     3421 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     3481 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     3541 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     3601 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     3661 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     3721 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     3781 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     3841 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     3901 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     3961 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     4021 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     4081 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     4141 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     4201 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     4261 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     4321 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     4381 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     4441 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     4501 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     4561 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     4621 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     4681 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     4741 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     4801 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     4861 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     4921 aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga ttattgacta
     4981 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     5041 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     5101 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     5161 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     5221 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     5281 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     5341 tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc
     5401 caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg
     5461 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcg
//
