LOCUS       sequence-193710-        4966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express rabies virus CVS N2c strain phosphoprotein gene (N2cP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4966)
  TITLE     Genetic Neuroengineering Group Plasmids 2017
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAG-N2cP
FEATURES             Location/Qualifiers
     source          1..4966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     331..353
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     415..434
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(1450..1469)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1515..1570
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1569..1588)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(1931..1947)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1931..1947)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1944..1966)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1955..1971
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1979..1985,1986..2003,2004..2009))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2024..2045
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2104..2299
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2150..2285
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2305..2439
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2342..2361)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2396..2415
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2507..2524)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2678..3266)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2758..2777)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3437..4228,4229..4297))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4060..4079
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4298..4402)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4433..4812
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
       61 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
      121 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
      181 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
      241 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg
      301 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
      361 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
      421 tgctggttat tgtgctgtct catcattttg gcaaagaatt cgccgccacc atgagcaaga
      481 tctttgttaa tccgagtgca atcagagccg gtctggccga tcttgagatg gccgaagaga
      541 ctgttgatct gatcaacaga aacatagaag acaatcaggc tcatctccag ggagaaccca
      601 tagaagtgga caacctccct gaggacatga agcgacttca cctggacgat gaaaaatcgt
      661 ccaaccttgg tgagatggtt agggtgggag aaggcaagta tcgagaggac tttcagatgg
      721 atgagggaga ggaccccaac ctcctgttcc aatcgtacct ggataatgtt ggagtccaaa
      781 tagtcagaca aatgaggtca ggagagagat tcctcaagat atggtcacag accgtagagg
      841 aaattgtatc ctatgtcacg gtcaactttc ctaaccctcc aagaaggtct tcggaggata
      901 aatcaaccca gactactggc agagagctca agaaggagac aacgtctgct ttctctcaga
      961 gagaaagcca accttcgaaa gctaggatgg tggctcaagt tgcccctggt cctccagccc
     1021 ttgaatggtc agccaccaat gaagaagatg atctatcagt agaggctgag atcgctcatc
     1081 agattgctga aagcttttcc aagaagtaca agtttccctc ccgatcttca ggaatattct
     1141 tgtataattt tgagcaactg aagatgaacc ttgatgacat agttaaagag gcaaaaaatg
     1201 taccgggcgt gacccgtctg gcccatgatg gatccaaaat ccccctgaga tgcgtactgg
     1261 gatgggtcgc tttggctaat tccaaaaaat tccaattact agtcgaggct gacaagctaa
     1321 gcaaaatcat gcaagatgat ttgaatcgct atacatcctg ctaagcggcc gcactcctca
     1381 ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata
     1441 ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca
     1501 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     1561 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta
     1621 tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct
     1681 ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa
     1741 agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac
     1801 atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct cctgactact
     1861 cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagcttgg
     1921 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     1981 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     2041 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga
     2101 tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     2161 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     2221 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     2281 aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa
     2341 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     2401 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc
     2461 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     2521 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     2581 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2641 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2701 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2761 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2821 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     2881 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2941 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3001 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     3061 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     3121 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3181 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3241 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     3301 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     3361 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3421 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     3481 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     3541 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     3601 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3661 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3721 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     3781 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3841 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     3901 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     3961 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     4021 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     4081 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     4141 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     4201 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     4261 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     4321 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     4381 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga
     4441 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4501 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4561 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4621 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4681 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4741 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4801 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc
     4861 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg
     4921 gggggggggg gggcgcgcgc caggcggggc ggggcggggc gagggg
//
