LOCUS       sequence-193712-        5637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express rabies virus CVS N2c strain glycoprotein gene (N2cG).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5637)
  TITLE     Genetic Neuroengineering Group Plasmids 2017
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAG-N2cG
FEATURES             Location/Qualifiers
     source          1..5637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     326..348
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     410..429
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      460..469
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2126..2145)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2191..2246
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2245..2264)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2607..2623)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2607..2623)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2620..2642)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2631..2647
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2655..2661,2662..2679,2680..2685))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2700..2721
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2780..2975
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2826..2961
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2981..3115
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3018..3037)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3072..3091
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3183..3200)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3354..3942)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3434..3453)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4113..4904,4905..4973))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4736..4755
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4974..5078)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5109..5488
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg
       61 gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg
      121 tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc
      181 ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg
      241 ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc
      301 cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga
      361 gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
      421 gttattgtgc tgtctcatca ttttggcaaa gaattcgccg ccaccatggt tcctcaggtt
      481 cttttgtttg tactccttct gggtttttcg ttgtgtttcg ggaagttccc catttacacg
      541 ataccagacg aacttggtcc ctggagccct attgacatac accatctcag ctgtccaaat
      601 aacctggttg tggaggatga aggatgtacc aacctgtccg agttctccta catggaactc
      661 aaagtgggat acatctcagc catcaaagtg aacgggttca cttgcacagg tgttgtgaca
      721 gaggcagaga cctacaccaa ctttgttggt tatgtcacaa ccacattcaa gagaaagcat
      781 ttccgcccca ccccagacgc atgtagagcc gcgtataact ggaagatggc cggtgacccc
      841 agatatgaag agtccctaca caatccatac cccgactacc actggcttcg aactgtaaga
      901 accaccaaag agtccctcat tatcatatcc ccaagtgtga cagatttgga cccatatgac
      961 aaatcccttc actcaagggt cttccctggc ggaaagtgct caggaataac ggtgtcctct
     1021 acctactgct caactaacca tgattacacc atttggatgc ccgagaatcc gagaccaagg
     1081 acaccttgtg acatttttac caatagcaga gggaagagag catccaacgg gaacaagact
     1141 tgcggctttg tggatgaaag aggcctgtat aagtctctaa aaggagcatg caggctcaag
     1201 ttatgtggag ttcttggact tagacttatg gatggaacat gggtcgcgat gcaaacatca
     1261 gatgagacca aatggtgccc tccagatcag ttggtgaatt tgcacgactt tcgctcagac
     1321 gagattgagc atctcgttgt ggaggagtta gtcaagaaaa gagaggaatg tctggacgca
     1381 ttagagtcca tcatgaccac caagtcagta agtttcagac gtctcagtca cctgagaaaa
     1441 cttgtcccag ggtttggaaa agcatatacc atattcaaca aaaccttgat ggaggctgat
     1501 gctcactaca agtcagtccg gacctggaat gagatcatcc cctcaaaagg gtgtttgaaa
     1561 gttggaggaa ggtgccatcc tcatgtgaac ggggtgtttt tcaatggtat aatattaggg
     1621 cctgacgacc atgtcctaat cccagagatg caatcatccc tcctccagca acatatggag
     1681 ttgttggaat cttcagttat ccccctgatg caccccctgg cagacccttc tacagttttc
     1741 aaagaaggtg atgaggctga ggattttgtt gaagttcacc tccccgatgt gtacaaacag
     1801 atctcagggg ttgacctggg tctcccgaac tggggaaagt atgtattgat gactgcaggg
     1861 gccatgattg gcctggtgtt gatattttcc ctaatgacat ggtgcagaag agccaatcga
     1921 ccagaatcga aacaacgcag ttttggaggg acagggggga atgtgtcagt cacttcccaa
     1981 agcggaaaag tcataccttc atgggaatca tataagagtg gaggtgagat cagactgtga
     2041 gcggccgcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg
     2101 ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca
     2161 tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag
     2221 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa
     2281 aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca
     2341 tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt
     2401 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt
     2461 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc
     2521 tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc
     2581 tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     2641 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     2701 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     2761 ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc
     2821 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     2881 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     2941 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta
     3001 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     3061 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg
     3121 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     3181 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     3241 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     3301 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     3361 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     3421 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     3481 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     3541 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     3601 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     3661 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     3721 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3781 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3841 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     3901 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     3961 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     4021 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     4081 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     4141 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     4201 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     4261 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     4321 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     4381 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     4441 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     4501 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     4561 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     4621 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     4681 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     4741 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     4801 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     4861 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     4921 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     4981 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     5041 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     5101 cctgggtcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     5161 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     5221 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     5281 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     5341 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     5401 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     5461 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct
     5521 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
     5581 tgcagcgatg ggggcggggg gggggggggg gcgcgccagg cggggcgggg cggggcg
//
