LOCUS       sequence-193713-       10417 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express rabies virus CVS N2c strain polymerase gene (N2cL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10417)
  TITLE     Genetic Neuroengineering Group Plasmids 2017
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAG-N2cL
FEATURES             Location/Qualifiers
     source          1..10417
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     297..319
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     381..400
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2081..2092
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(3884..3895)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6906..6925)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6971..7026
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7025..7044)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7387..7403)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7387..7403)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7400..7422)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7411..7427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7435..7441,7442..7459,7460..7465))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7480..7501
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7560..7755
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7606..7741
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7761..7895
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7798..7817)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7852..7871
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7963..7980)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8134..8722)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8214..8233)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8893..9684,9685..9753))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9516..9535
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9754..9858)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        9889..10268
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
       61 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
      121 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
      181 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
      241 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
      301 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt
      361 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
      421 agaattcgcc gccaccatgc tagatccggg agaggtttat gatgacccta ttgatccaat
      481 tgagtcagag gctgaaccca gaggaacccc cactgtcccc aacatcttga ggaactccga
      541 ctacaatctc aattctcctt tgatagagga tcctgccaaa ctaatgttag aatggttgaa
      601 gacagggaac agaccttatc ggatgacttt gacagacaat tgctccaggt cttacaaagt
      661 tttgaaagat tatttcaaga aagtagattt gggttctctc aaagtgggcg gaactgctgc
      721 acagtcaatg gtttctctct ggttgtgtgg tgcccactct gaatcaaaca ggagccggag
      781 atgtataacc gacttggccc atttctattc caagtcatcc cccatagaga agctattgaa
      841 ttgtacgcta ggaaacagag gcctgagaat cccaccagag ggggtgttaa attgcctcga
      901 gagggtcaat tatgacaagg catttgggag gtatctggcc aacacgtatt cctcttactt
      961 gtttttccat gtaatcacct tatacatgaa tgccttagac tgggaagagg aaaagaccat
     1021 cctggcatta tggaaagata taacctcagt ggataccgag aaggacttgg tcaaattcaa
     1081 agatcaaata tggggactgt tgattgtgac aaaggacttt gtttactctc agagttcaaa
     1141 ctgtcttttt gacagaaact acacactgat gctaaaggat cttttcttgt ctcgattcaa
     1201 ctccttaatg attctgcttt ctccccctga gccccgatac tcagatgact taatatctca
     1261 gctgtgccag ctatacatcg ctggggatca agtcttgtcc atgtgtggga actccggcta
     1321 tgaagtcatc aaaatattgg agccatatgt cgtgaacagt ttggtccaga gggcagagaa
     1381 gtttaggcct ctcatccacc ccttgggaga ctttcctatg ttcataaaag acaaggtgaa
     1441 tcaacttgaa gggacttttg gtcccagtgc aaagaggttt tttagggttt tagatcaatt
     1501 cgacaacata catgacttag tatttgtgta tggctgttac agacattggg ggcaccccta
     1561 tatagattat cggaagggtc tgtcgaaact atatgatcaa gttcacatta agaaagtaat
     1621 agataagtcc taccaggagt gtttagcaag tgacttggcc agaaggatcc tcagatgggg
     1681 atttgacaag tactccaaat ggtatctaga ttcgagattc cttgcccgag accacccctt
     1741 gactccttat gtcaagaccc aaacatggcc acccaaacat atagtagact tggtggggga
     1801 cacatggcat aagctcccga tcacgcagat ctttgaaatt cctgaatcaa tggacccgtc
     1861 agagatactg gatgacaaat cacattcttt caccagaaca aggttagctt cttggctgtc
     1921 agagaaccga ggggggcctg ttcctagcga gaaggtcatt atcacggccc tgtctaagcc
     1981 acctgtcaat ccccgagagt ttttgaaatc tatagacctc ggaggattgc cagatgagga
     2041 tttgataatt ggcctcaaac caaaggaacg ggagttgaag atcgagggcc gattctttgc
     2101 tctaatgtca tggaatctaa gattatattt tgtcatcacc gaaaagctct tggccaacta
     2161 cattttgcca ctttttgacg cactgactat gacagacaac ctgaacaagg tgttcaaaaa
     2221 gttgatcgac agggtcaccg ggcaagggct tttggactat tctagggtca catacgcatt
     2281 tcacctggac tatgagaaat ggaacaatca tcaaagattg gagtcaacag aggatgtatt
     2341 ctctgtccta gatcaggtgt ttggattgaa gagggtgttt tctagaacac acgagttttt
     2401 tcagaagtcc tggatctatt attcagacag atcagacctc attgggttat gggaggacca
     2461 aatatattgc ttggatatgt ctaacggccc aacctgctgg aatggccaag atggcgggct
     2521 agagggcttg cggcagaagg gctggagtct agtcagttta ttaatgatag atagagaatc
     2581 tcaaaccagg aacacaagaa ccaagatact agctcaagga gacaaccagg ttctgtgtcc
     2641 tacatacatg ttgtcaccgg gattgtctca agaggggctt ctctatgagt tagagagcat
     2701 atcaaggaat gcactctcaa tataccgagc tatcgaggaa ggagcatcta agctggggct
     2761 gatcatcaag aaggaagaga ccatgtgtag ttatgacttt ctcatatatg ggaagacccc
     2821 cttatttcga ggcaacatat tggtgcctga atccaaaaga tgggcccgag tctcttgcat
     2881 ctctaacgac caaatagtca acctcgccaa tataatgtcg acagtatcca ccaatgcgct
     2941 gacagtggca caacactctc aatctctgat caaacctatg agggattttc tgctcatgtc
     3001 agtacaggca gttttccact acctgttgtt tagcccaatc ctaaaaggca gagtttataa
     3061 gattctgagt gctgaagggg agagctttct cctagccatg tcgcggataa tctacctaga
     3121 tccttctttg ggaggggtgt ctggaatgtc tctcgggagg ttccatatac gtcagttctc
     3181 agaccctgtc tctgaagggt tgtcattctg gagagagatc tggttaggct ctcatgagtc
     3241 ctggattcac gcgttgtgtc aggaggccgg gaaccccgat cttggagaga gaacactcga
     3301 gagcttcact cgccttttag aagatcctac taccttaaat atcaaaggag gggccagccc
     3361 taccattcta ctcaaggatg ctatcagaaa ggctctgtac gacgaggtgg acaaggtgga
     3421 aaattcagag tttcgagagg caatcctgct gtccaagacc catagagata actttatact
     3481 ctttttaaaa tctgttgagc ctctgttccc tcgatttctc agtgagctct tcagttcgtc
     3541 tttcttggga ataccggagt caatcattga actgatacaa aactcccgga caataagaag
     3601 gcagtttaga aagagtctct caagaacttt agaagagtcc ttctacaact cagagatcca
     3661 cgggattaat cggataaccc agacacctca aagggtcgga agggtgtggc cttgctcttc
     3721 agagagggca gatctactta gggagatctc ttgggggagg aaagtggtag gcacgacagt
     3781 tcctcaccct tccgagatgt tggagttgtt tcccaaatcc tccatttcct gcacttgtgg
     3841 agcaacaggg ggaggcaatc ctagagtctc tgtatcagta ctcccgtcct tcgatcagtc
     3901 atttttctca cggggccccc taaagggata cttgggctcg tccacctcca tgtcaaccca
     3961 gctattccat gcatgggaaa aagtcactaa tgttcatgtg gtgaaaaggg ctctatcgtt
     4021 aaaagaatct ataaactggt tcatcaatag gaattccaat ttggctcaaa ctctaattgg
     4081 aaacatcatg tctctgacag gccctgattt ccctctagaa gaggcccctg ttttcaaacg
     4141 gacagggtca gccttgcata ggttcaagtc tgccagatac agcgaaggag ggtattcttc
     4201 tgtttgccct aaccttctct cccatatctc tgttagtaca gacactatgt ctgatttgac
     4261 ccaaaacggg aagaactatg atttcatgtt tcagccattg atgctttatg cgcaaacatg
     4321 gacatcggaa ctggtacaga gggatacaag acttagagac tccacgtttc actggcacct
     4381 tcggtgcaac agatgtgtga ggcccattga tgatataaca ctggaaactt ctcagatctt
     4441 cgagttcccg gatgtgtcaa aaaggatatc caggatggtt tctggagctg tccctcaatt
     4501 tcagaagctt cctgatatcc gtctaagacc aggtgatttt gaatccctaa gtggtagaga
     4561 aaagtctcgc catatagggt cagctcaggg gctcttatac tcaatcttag tagcaattca
     4621 cgactcagga tacaatgatg ggaccatctt ccctgtcaac atatacggca aagtttcccc
     4681 cagagactat ttgagagggc ttgcaagagg gatcttgata gggtcctcga tttgcttctt
     4741 gacacgaatg acaaatatta acattaacag acctcttgaa ttgatctcag gggtaatttc
     4801 ctatattctc ctgaggctgg ataatcatcc ctctctgtat ataatgctta gagaaccgtc
     4861 tcttagagga gaaatattct ctatccctca gaaaatcccc gccgcttacc caaccactat
     4921 gagagaaggc aacagatcga ttttgtgtta cctccaacac gtgctacgct atgagcgaga
     4981 ggcaatcacg gcgtccccgg agaatgactg gctgtggatc ttctcagact tcagaagtgt
     5041 gaaaatgacg tacttgaccc tcattaccta ccagtctcac ctcctactcc agagggttga
     5101 gagaaacttg tctaagagta tgagagctac tctgcgacaa atgagttcct taatgaggca
     5161 agtgctgggt gggcacggag aagatacctt ggagtcagac gatgacattc aacgattact
     5221 aaaagactct ttgcgaaaga caaggtgggt ggatcaagag gtgcgccatg cagctagaac
     5281 catgaatgga gattacagcc ccgacaagaa agtatcccac aaggcaggat gttcagaatg
     5341 ggtctgctct gctcaacaga ttgccgtctc cacctcagcc aacccggccc ctgtctcaga
     5401 gcttgacatt agggccctct ctaagaggtt tcaaaacccc ttgatctcgg gcctgagagt
     5461 ggttcagtgg gcaaccggtg cccattataa gcttaagcct attctagatg atctaaatgt
     5521 tttcccatct ctctgtcttg taattggaga cgggtcaggg ggaatatcaa gggcagttct
     5581 caacatgttt ccagattcta agcttgtgtt caacagccta ttggaggtga atgatctgat
     5641 ggcttccgga acacatccac tgcctccttc agcaatcatg agtggaggag atgacatcat
     5701 ctccagagtg atagactttg actcaatatg ggagaaaccg tccgacctga ggaactcggc
     5761 cacctggaga tacttccagt cggttcaaaa acaggtcaac atgtcgtatg acctcattat
     5821 ttgtgatgca gaagttactg atattgcatc tatcaaccgg ataactctgt tgatgtctga
     5881 tttcgcattg tctatagatg gaccacttta tctggtcttc aaaacttacg ggactatgct
     5941 agtaaacccg gactataaag ctattcaaca tctgtcaaga gcgttccctt cggtcacagg
     6001 gtttgtaacc caagtaactt catccttttc ttctgagcta tacctccggt tctctaaacg
     6061 aggaaagttt ttcagggacg ccgagtactt gacctcttcc acccttcgag agatgagcct
     6121 tgtgttgttc aattgcagca gccccaaaag tgagatgcag agagctcgtt ccttaaacta
     6181 tcaagacctg gtaaggggat ttcctgaaga gatcatatca aatccttaca acgaaatgat
     6241 cataactctg attgacaatg atgtagagtc cttcctagtc cacaagatgg tggatgatct
     6301 tgagctacag aggggaactc tgtctaaagt ggctatcatt atatccatca tgatcgtttt
     6361 ttccaataga gtcttcaaca tttccaaacc tttgactgac cccttgttct accccccatc
     6421 tgatcctaaa atcctgaggc acttcaacat atgttgcagt actatgatgt atctatctac
     6481 cgctttaggc gacgtcccta acttcgcaag acttcatgac ctgtataaca gacctataac
     6541 ttgttacttc agaaaacaag ttattcgagg gaatatttat ctatcttgga gttggtccga
     6601 tgatacccca gtgttcaaga gagtagcctg taattctagc ttgagtctgt catctcactg
     6661 gatcaggttg atctacaaga tagtgaagac taccagactc attggcagca taaaagacct
     6721 atcaggagag gtagaacgac atcttcatgg gtataacaga tggatcaccc tcgaggatat
     6781 ccgatctaga tcatccctac tagattacag ttgtttgtaa gcggccgcac tcctcaggtg
     6841 caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac
     6901 tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg
     6961 acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt
     7021 ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg
     7081 gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa
     7141 agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc
     7201 ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc
     7261 ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca
     7321 gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa gcttggcgta
     7381 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7441 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     7501 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg
     7561 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     7621 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7681 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7741 ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac aaataaagca
     7801 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7861 ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa tcggccaacg
     7921 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     7981 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     8041 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     8101 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     8161 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     8221 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     8281 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     8341 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8401 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8461 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8521 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     8581 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8641 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     8701 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     8761 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     8821 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     8881 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     8941 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     9001 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     9061 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     9121 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     9181 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     9241 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9301 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     9361 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     9421 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     9481 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     9541 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9601 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9661 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     9721 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     9781 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     9841 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga cattgattat
     9901 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     9961 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
    10021 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
    10081 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
    10141 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
    10201 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
    10261 ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc
    10321 cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg
    10381 gggggggggc gcgcgccagg cggggcgggg cggggcg
//
