LOCUS       sequence-193742-        9868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression of fluorescent sensor reporting the activity of 
            ammonium transceptors. Contains a stable reference LSSmOrange nested
            within the reporting superfolder cpEGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Ast C, Foret J, Oltrogge LM, De Michele R, Kleist TJ, Ho CH, Frommer
            WB
  TITLE     Ratiometric Matryoshka biosensors from a nested cassette of green- 
            and orange-emitting fluorescent proteins.
  JOURNAL   Nat Commun. 2017 Sep 5;8(1):431. doi: 10.1038/s41467-017-00400-2.
  PUBMED    28874729
REFERENCE   2  (bases 1 to 9868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-09-5"; volume: "8(1):431. doi"; pages: " 
            10.1038/s41467-017-00400-2"
COMMENT     Sequence Label: pDRF'- AmTryoshka1;3-LS-F138I-T78H
FEATURES             Location/Qualifiers
     source          1..9868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             982..1689
                     /codon_start=1
                     /product="monomeric orange fluorescent protein with a large
                     Stokes shift (Shcherbakova et al., 2012)"
                     /label=LSSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTVKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGMEASSERMYPEDGALK
                     GEDKLRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1395..1414
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(2933..2957)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3019..3038)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3020..3038)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      3186..3373
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3241..3265)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(3385..3402)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3385..3401)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3394..3416)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3837..5183
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(5294..6097)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6098..6313)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(6338..6356)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6424..6528
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6529..6597,6598..7389)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6747..6766)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7560..8148
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8049..8068
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8302..8319
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8436..8457
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8472..8477,8478..8495,8496..8502)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8510..8526
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8515..8537
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8534..8550
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8534..8550
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(8580..9035)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8717..8738)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8929..8948
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    9842..9866
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgtcaggag caataacatg ctctgcggcc gatctcgcca ccctacttgg ccccaacgcc
       61 acggcggcgg ccgactacat ttgcggccaa ttaggcaccg ttaacaacaa gttcaccgat
      121 gcagccttcg ccatagacaa cacctacctc ctcttctctg cctaccttgt cttcgccatg
      181 cagctcggct tcgctatgct ttgtgctggt tctgttagag ccaagaatac gatgaacatc
      241 atgcttacca atgtccttga cgctgcagcc ggaggactct tctactatct ctttggttac
      301 gcctttgcct ttggaggatc ctccgaaggg ttcattggaa gacacaactt tgctcttaga
      361 gactttccga ctcccacagc tgattactct ttcttcctct accaatgggc gatcgcaatc
      421 gcggccgctg gaatcacaag tggttcgatc gcagagagga ctcagttcgt ggcttacttg
      481 atatactctt ctttcttaac cggatttgtt tacccggttg tctctcactg gttttggtcc
      541 ccggatggat gggccagtcc ctttcgttca gcggatgatc gtttgtttag caccggagcc
      601 attgactttg ctggctccgg tgttgttcac atggttggtg gcatagcagg tttatggggt
      661 gctcttattg aaggtcctcg tcgtggtcgg ttcgagaaat tgtccaacgt gtatattacc
      721 gcggataaac agaaaaacgg cattaaagcg aactttcacg tgcgccataa cgtggaagat
      781 ggcagcgtgc agctggcgga tcattatcag cagaacaccc cgattggcga tggcccggtg
      841 ctgctgccgg ataaccatta tctgagcacc cagaccaagc tgagcaaaga tccgaacgaa
      901 aaacgcgatc acatggtgct gctggaattt gtgaccgcag cgggcattac acacggcatg
      961 gatgaactgt atggcggcac catggtgagc aagggcgagg agaataacat ggccatcatc
     1021 aaggagttca tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca cgagttcgag
     1081 atcgagggcg agggcgaggg ccgcccctac gagggctttc agaccgttaa gctgaaggtg
     1141 accaagggtg gccccctgcc cttcgcctgg gacatcttgt cccctcagtt cacctacggc
     1201 tccaaggcct acgtgaagca ccccgccgac atccccgact acctcaagct gtccttcccc
     1261 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgact
     1321 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     1381 ttcccctccg acggccccgt aatgcagaag aagaccatgg gcatggaggc ctcctccgag
     1441 cggatgtacc ccgaggacgg cgccctgaag ggcgaggaca agctcaggct gaagctgaag
     1501 gacggcggcc actacacctc cgaggtcaag accacctaca aggccaagaa gcccgtgcag
     1561 ttgcccggcg cctacatcgt cgacatcaag ttggacatca cctcccacaa cgaggactac
     1621 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     1681 ctgtacaagg gcggcagcgc gagccagggc gaagaactgt ttaccggcgt ggtgccgatt
     1741 ctggtggaac tggatggcga tgtgaacggc cataaattta gcgtgcgcgg cgaaggcgaa
     1801 ggcgatgcga ccattggcaa actgaccctg aaatttattt ccaccaccgg caaactaccg
     1861 gtgccgtggc cgaccctggt gaccacctta acctatggcg tgcagtgctt tagccgctat
     1921 ccggatcata tgaaacgcca tgattttttt aaaagcgcga tgccggaagg ctatgtgcag
     1981 gaacgcacca ttagctttaa agatgatggc aaatataaaa cccgcgcggt ggtgaaattt
     2041 gaaggcgata ccctggtgaa ccgcattgaa ctgaaaggca ccgattttaa agaagatggc
     2101 aacattctgg ggcataaact ggaatataac tttaatggtg gtcgcgctat tgctctgcgc
     2161 ggccactctg cctcgctagt agtcttagga accttcctcc tatggtttgg atggtatggt
     2221 ttcaaccccg gttccttcac taagatactc gttccgtata attctggttc caactacggc
     2281 caatggagcg gaatcggccg tacagcggtt aacaccacac tctcaggatg cacagcagct
     2341 ctaaccacac tctttggtaa acgtctccta tcaggccact ggaacgtaac ggacgtttgc
     2401 aacgggttac tcggtgggtt tgcggccata accgcaggtt gctccgtcgt agagccatgg
     2461 gcagcgattg tgtgcggctt catggcttct gtcgtcctta tcggatgcaa caagctcgcg
     2521 gagcttgtac aatatgatga tccactcgag gcagcccaac tacatggagg gtgtggcgcg
     2581 tgggggttga tattcgtagg attgtttgcc aaagagaagt atctaaacga ggtttatggc
     2641 gccaccccgg gaaggccata tggactattt atgggcggag gagggaagct gttgggagca
     2701 caattggttc aaatacttgt gattgtagga tgggttagtg ccacaatggg aacactcttc
     2761 ttcatcctca aaaggctcaa tctgcttagg atctcggagc agcatgaaat gcaagggatg
     2821 gatatgacac gtcacggtgg ctttgcttat atctaccatg ataatgatga tgagtctcat
     2881 agagtggatc ctggatctcc tttccctcga tcagctactc ctcctcgcgt tgacccagct
     2941 ttcttgtaca aagtggtgta gttaattcat gatggccgct gcaggtcgac ctcgaggggg
     3001 ggcccggtac ccaattcgcc ctatagtgag tcgtattacg cgcggatcca gctttggact
     3061 tcttcgccag aggtttggtc aagtctccaa tcaaggttgt cggcttgtct accttgccag
     3121 aaatttacga aaagatggaa aagggtcaaa tcgttggtag atacgttgtt gacacttcta
     3181 aataagcgaa tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa
     3241 gtgtatacaa attttaaagt gactcttagg ttttaaaacg aaaattctta ttcttgagta
     3301 actctttcct gtaggtcagg ttgctttctc aggtatagca tgaggtcgct cttattgacc
     3361 acacctctac cggcatgcca attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     3421 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     3481 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     3541 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcataatcgg
     3601 atcgtacttg ttacccatca ttgaattttg aacatccgaa cctgggagtt ttccctgaaa
     3661 cagatagtat atttgaacct gtataataat atatagtcta gcgctttacg gaagacaatg
     3721 tatgtatttc ggttcctgga gaaactattg catctattgc ataggtaatc ttgcacgtcg
     3781 catccccggt tcattttctg cgtttccatc ttgcacttca atagcatatc tttgttaacg
     3841 aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa
     3901 caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgaaagcg ctattttacc
     3961 aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt
     4021 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga gagcgctatt
     4081 ttaccaacaa agaatctata cttctttttt gttctacaaa aatgcatccc gagagcgcta
     4141 tttttctaac aaagcatctt agattacttt ttttctcctt tgtgcgctct ataatgcagt
     4201 ctcttgataa ctttttgcac tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc
     4261 tattttctct tccataaaaa aagcctgact ccacttcccg cgtttactga ttactagcga
     4321 agctgcgggt gcattttttc aagataaagg catccccgat tatattctat accgatgtgg
     4381 attgcgcata ctttgtgaac agaaagtgat agcgttgatg attcttcatt ggtcagaaaa
     4441 ttatgaacgg tttcttctat tttgtctcta tatactacgt ataggaaatg tttacatttt
     4501 cgtattgttt tcgattcact ctatgaatag ttcttactac aatttttttg tctaaagagt
     4561 aatactagag ataaacataa aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg
     4621 aaaggtggat gggtaggtta tatagggata tagcacagag atatatagca aagagatact
     4681 tttgagcaat gtttgtggaa gcggtattcg caatatttta gtagctcgtt acagtccggt
     4741 gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt tggttttcaa aagcgctctg
     4801 aagttcctat actttctagc tagagaatag gaacttcgga ataggaactt caaagcgttt
     4861 ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc acatacagct cactgttcac
     4921 gtcgcaccta tatctgcgtg ttgcctgtat atatatatac atgagaagaa cggcatagtg
     4981 cgtgtttatg cttaaatgcg tacttatatg cgtctattta tgtaggatga aaggtagtct
     5041 agtacctcct gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta
     5101 ccctttagct gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct
     5161 atcatttcct ttgatattgg atcgatccga tgataagctg tcaaacatga gaattgggta
     5221 ataactgata taattaaatt gaagctctaa tttgtgagtt tagtatacat gcatttactt
     5281 ataatacagt tttttagttt tgctggccgc atcttctcaa atatgcttcc cagcctgctt
     5341 ttctgtaacg ttcaccctct accttagcat cccttccctt tgcaaatagt cctcttccaa
     5401 caataataat gtcagatcct gtagagacca catcatccac ggttctatac tgttgaccca
     5461 atgcgtctcc cttgtcatct aaacccacac cgggtgtcat aatcaaccaa tcgtaacctt
     5521 catctcttcc acccatgtct ctttgagcaa taaagccgat aacaaaatct ttgtcgctct
     5581 tcgcaatgtc aacagtaccc ttagtatatt ctccagtaga tagggagccc ttgcatgaca
     5641 attctgctaa catcaaaagg cctctaggtt cctttgttac ttcttctgcc gcctgcttca
     5701 aaccgctaac aatacctggg cccaccacac cgtgtgcatt cgtaatgtct gcccattctg
     5761 ctattctgta tacacccgca gagtactgca atttgactgt attaccaatg tcagcaaatt
     5821 ttctgtcttc gaagagtaaa aaattgtact tggcggataa tgcctttagc ggcttaactg
     5881 tgccctccat ggaaaaatca gtcaagatat ccacatgtgt ttttagtaaa caaattttgg
     5941 gacctaatgc ttcaactaac tccagtaatt ccttggtggt acgaacatcc aatgaagcac
     6001 acaagtttgt ttgcttttcg tgcatgatat taaatagctt ggcagcaaca ggactaggat
     6061 gagtagcagc acgttcctta tatgtagctt tcgacatgat ttatcttcgt ttcctgcatg
     6121 tttttgttct gtgcagttgg gttaagaata ctgggcaatt tcatgtttct tcaacactac
     6181 atatgcgtat atataccaat ctaagtctgt gctccttcct tcgttcttcc ttctgttcgg
     6241 agattaccga atcaaaaaaa tttcaaggaa accgaaatca aaaaaaagaa taaaaaaaaa
     6301 atgatgaatt gaaaagctaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     6361 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     6421 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     6481 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     6541 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     6601 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     6661 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     6721 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     6781 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     6841 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     6901 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     6961 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     7021 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     7081 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     7141 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     7201 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     7261 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     7321 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     7381 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     7441 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     7501 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     7561 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     7621 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7681 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     7741 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7801 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7861 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7921 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7981 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     8041 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     8101 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     8161 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     8221 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     8281 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     8341 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     8401 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     8461 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     8521 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttac cgcatcagga
     8581 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     8641 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     8701 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     8761 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     8821 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     8881 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     8941 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     9001 acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccaagctt cctgaaacgg
     9061 agaaacataa acaggcattg ctgggatcac ccatacatca ctctgttttg cctgaccttt
     9121 tccggtaatt tgaaaacaaa cccggtctcg aagcggagat ccggcgataa ttaccgcaga
     9181 aataaaccca tacacgagac gtagaaccag ccgcacatgg ccggagaaac tcctgcgaga
     9241 atttcgtaaa ctcgcgcgca ttgcatctgt atttcctaat gcggcacttc caggcctcga
     9301 tcgagaccgt ttatccattg cttttttgtt gtctttttcc ctcgttcaca gaaagtctga
     9361 agaagctata gtagaactat gagctttttt tgtttctgtt ttcctttttt tttttttacc
     9421 tctgtggaaa ttgttactct cacactcttt agttcgtttg tttgttttgt ttattccaat
     9481 tatgaccggt gacgaaacgt ggtcgatggt gggtaccgct tatgctcccc tccattagtt
     9541 tcgattatat aaaaaggcca aatattgtat tattttcaaa tgtcctatca ttatcgtcta
     9601 acatctaatt tctcttaaat tttttctctt tctttcctat aacaccaata gtgaaaatct
     9661 ttttttcttc tatatctaca aaaacttttt ttttctatca acctcgttga taaatttttt
     9721 ctttaacaat cgttaataat taattaattg gaaaataacc attttttctc tcttttatac
     9781 acacattcaa aagaaagaaa aaaaatatac cccagcctcg actagtgaat tcgcggccat
     9841 cacaagtttg tacaaaaaag caggcttt
//
