LOCUS       sequence-193745-        9868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast expression of fluorescent sensor reporting the activity of 
            ammonium transceptors. Contains a stable reference LSSmOrange nested
            within the reporting superfolder cpEGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Ast C, Foret J, Oltrogge LM, De Michele R, Kleist TJ, Ho CH, Frommer
            WB
  TITLE     Ratiometric Matryoshka biosensors from a nested cassette of green- 
            and orange-emitting fluorescent proteins.
  JOURNAL   Nat Commun. 2017 Sep 5;8(1):431. doi: 10.1038/s41467-017-00400-2.
  PUBMED    28874729
REFERENCE   2  (bases 1 to 9868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-09-5"; volume: "8(1):431. doi"; pages: " 
            10.1038/s41467-017-00400-2"
COMMENT     Sequence Label: pDRF'- AmTryoshka1;3-GS-F138I
FEATURES             Location/Qualifiers
     source          1..9868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    425..449
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1433..2140
                     /codon_start=1
                     /product="monomeric orange fluorescent protein with a large
                     Stokes shift (Shcherbakova et al., 2012)"
                     /label=LSSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTVKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGMEASSERMYPEDGALK
                     GEDKLRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1846..1865
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(3384..3408)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3470..3489)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3471..3489)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      3637..3824
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3692..3716)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(3836..3853)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3836..3852)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3845..3867)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4288..5634
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(5745..6548)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6549..6764)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(6789..6807)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6875..6979
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6980..7048,7049..7840)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7198..7217)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8011..8599
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8500..8519
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8753..8770
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8887..8908
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8923..8928,8929..8946,8947..8953)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8961..8977
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8966..8988
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8985..9001
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8985..9001
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(9031..9486)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9168..9189)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9380..9399
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 acctctgtgg aaattgttac tctcacactc tttagttcgt ttgtttgttt tgtttattcc
       61 aattatgacc ggtgacgaaa cgtggtcgat ggtgggtacc gcttatgctc ccctccatta
      121 gtttcgatta tataaaaagg ccaaatattg tattattttc aaatgtccta tcattatcgt
      181 ctaacatcta atttctctta aattttttct ctttctttcc tataacacca atagtgaaaa
      241 tctttttttc ttctatatct acaaaaactt tttttttcta tcaacctcgt tgataaattt
      301 tttctttaac aatcgttaat aattaattaa ttggaaaata accatttttt ctctctttta
      361 tacacacatt caaaagaaag aaaaaaaata taccccagcc tcgactagtg aattcgcggc
      421 catcacaagt ttgtacaaaa aagcaggctt tatgtcagga gcaataacat gctctgcggc
      481 cgatctcgcc accctacttg gccccaacgc cacggcggcg gccgactaca tttgcggcca
      541 attaggcacc gttaacaaca agttcaccga tgcagccttc gccatagaca acacctacct
      601 cctcttctct gcctaccttg tcttcgccat gcagctcggc ttcgctatgc tttgtgctgg
      661 ttctgttaga gccaagaata cgatgaacat catgcttacc aatgtccttg acgctgcagc
      721 cggaggactc ttctactatc tctttggtta cgcctttgcc tttggaggat cctccgaagg
      781 gttcattgga agacacaact ttgctcttag agactttccg actcccacag ctgattactc
      841 tttcttcctc taccaatggg cgatcgcaat cgcggccgct ggaatcacaa gtggttcgat
      901 cgcagagagg actcagttcg tggcttactt gatatactct tctttcttaa ccggatttgt
      961 ttacccggtt gtctctcact ggttttggtc cccggatgga tgggccagtc cctttcgttc
     1021 agcggatgat cgtttgttta gcaccggagc cattgacttt gctggctccg gtgttgttca
     1081 catggttggt ggcatagcag gtttatgggg tgctcttatt gaaggtcctc gtcgtggtcg
     1141 gttcgagaaa ggtagtaacg tgtatattac cgcggataaa cagaaaaacg gcattaaagc
     1201 gaactttacc gtgcgccata acgtggaaga tggcagcgtg cagctggcgg atcattatca
     1261 gcagaacacc ccgattggcg atggcccggt gctgctgccg gataaccatt atctgagcac
     1321 ccagaccaag ctgagcaaag atccgaacga aaaacgcgat cacatggtgc tgctggaatt
     1381 tgtgaccgca gcgggcatta cacacggcat ggatgaactg tatggcggca ccatggtgag
     1441 caagggcgag gagaataaca tggccatcat caaggagttc atgcgcttca aggtgcgcat
     1501 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     1561 cgagggcttt cagaccgtta agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     1621 ggacatcttg tcccctcagt tcacctacgg ctccaaggcc tacgtgaagc accccgccga
     1681 catccccgac tacctcaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     1741 cttcgaggac ggcggcgtgg tgaccgtgac tcaggactcc tccctgcagg acggcgagtt
     1801 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     1861 gaagaccatg ggcatggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     1921 gggcgaggac aagctcaggc tgaagctgaa ggacggcggc cactacacct ccgaggtcaa
     1981 gaccacctac aaggccaaga agcccgtgca gttgcccggc gcctacatcg tcgacatcaa
     2041 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     2101 gggccgccac tccaccggcg gcatggacga gctgtacaag ggcggcagcg cgagccaggg
     2161 cgaagaactg tttaccggcg tggtgccgat tctggtggaa ctggatggcg atgtgaacgg
     2221 ccataaattt agcgtgcgcg gcgaaggcga aggcgatgcg accattggca aactgaccct
     2281 gaaatttatt tccaccaccg gcaaactacc ggtgccgtgg ccgaccctgg tgaccacctt
     2341 aacctatggc gtgcagtgct ttagccgcta tccggatcat atgaaacgcc atgatttttt
     2401 taaaagcgcg atgccggaag gctatgtgca ggaacgcacc attagcttta aagatgatgg
     2461 caaatataaa acccgcgcgg tggtgaaatt tgaaggcgat accctggtga accgcattga
     2521 actgaaaggc accgatttta aagaagatgg caacattctg gggcataaac tggaatataa
     2581 ctttaatggt ggtcgcgcta ttgctctgcg cggccactct gcctcgctag tagtcttagg
     2641 aaccttcctc ctatggtttg gatggtatgg tttcaacccc ggttccttca ctaagatact
     2701 cgttccgtat aattctggtt ccaactacgg ccaatggagc ggaatcggcc gtacagcggt
     2761 taacaccaca ctctcaggat gcacagcagc tctaaccaca ctctttggta aacgtctcct
     2821 atcaggccac tggaacgtaa cggacgtttg caacgggtta ctcggtgggt ttgcggccat
     2881 aaccgcaggt tgctccgtcg tagagccatg ggcagcgatt gtgtgcggct tcatggcttc
     2941 tgtcgtcctt atcggatgca acaagctcgc ggagcttgta caatatgatg atccactcga
     3001 ggcagcccaa ctacatggag ggtgtggcgc gtgggggttg atattcgtag gattgtttgc
     3061 caaagagaag tatctaaacg aggtttatgg cgccaccccg ggaaggccat atggactatt
     3121 tatgggcgga ggagggaagc tgttgggagc acaattggtt caaatacttg tgattgtagg
     3181 atgggttagt gccacaatgg gaacactctt cttcatcctc aaaaggctca atctgcttag
     3241 gatctcggag cagcatgaaa tgcaagggat ggatatgaca cgtcacggtg gctttgctta
     3301 tatctaccat gataatgatg atgagtctca tagagtggat cctggatctc ctttccctcg
     3361 atcagctact cctcctcgcg ttgacccagc tttcttgtac aaagtggtgt agttaattca
     3421 tgatggccgc tgcaggtcga cctcgagggg gggcccggta cccaattcgc cctatagtga
     3481 gtcgtattac gcgcggatcc agctttggac ttcttcgcca gaggtttggt caagtctcca
     3541 atcaaggttg tcggcttgtc taccttgcca gaaatttacg aaaagatgga aaagggtcaa
     3601 atcgttggta gatacgttgt tgacacttct aaataagcga atttcttatg atttatgatt
     3661 tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag
     3721 gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct
     3781 caggtatagc atgaggtcgc tcttattgac cacacctcta ccggcatgcc aattcactgg
     3841 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     3901 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     3961 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
     4021 atctgtgcgg tatttcacac cgcataatcg gatcgtactt gttacccatc attgaatttt
     4081 gaacatccga acctgggagt tttccctgaa acagatagta tatttgaacc tgtataataa
     4141 tatatagtct agcgctttac ggaagacaat gtatgtattt cggttcctgg agaaactatt
     4201 gcatctattg cataggtaat cttgcacgtc gcatccccgg ttcattttct gcgtttccat
     4261 cttgcacttc aatagcatat ctttgttaac gaagcatctg tgcttcattt tgtagaacaa
     4321 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
     4381 cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc atttttgtaa
     4441 aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta
     4501 cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat acttcttttt
     4561 tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct tagattactt
     4621 tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca ctgtaggtcc
     4681 gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa aaagcctgac
     4741 tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt caagataaag
     4801 gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa cagaaagtga
     4861 tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta ttttgtctct
     4921 atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac tctatgaata
     4981 gttcttacta caattttttt gtctaaagag taatactaga gataaacata aaaaatgtag
     5041 aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt atatagggat
     5101 atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga agcggtattc
     5161 gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt
     5221 cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag ctagagaata
     5281 ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg agcgcttccg aaaatgcaac
     5341 gcgagctgcg cacatacagc tcactgttca cgtcgcacct atatctgcgt gttgcctgta
     5401 tatatatata catgagaaga acggcatagt gcgtgtttat gcttaaatgc gtacttatat
     5461 gcgtctattt atgtaggatg aaaggtagtc tagtacctcc tgtgatatta tcccattcca
     5521 tgcggggtat cgtatgcttc cttcagcact accctttagc tgttctatat gctgccactc
     5581 ctcaattgga ttagtctcat ccttcaatgc tatcatttcc tttgatattg gatcgatccg
     5641 atgataagct gtcaaacatg agaattgggt aataactgat ataattaaat tgaagctcta
     5701 atttgtgagt ttagtataca tgcatttact tataatacag ttttttagtt ttgctggccg
     5761 catcttctca aatatgcttc ccagcctgct tttctgtaac gttcaccctc taccttagca
     5821 tcccttccct ttgcaaatag tcctcttcca acaataataa tgtcagatcc tgtagagacc
     5881 acatcatcca cggttctata ctgttgaccc aatgcgtctc ccttgtcatc taaacccaca
     5941 ccgggtgtca taatcaacca atcgtaacct tcatctcttc cacccatgtc tctttgagca
     6001 ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc cttagtatat
     6061 tctccagtag atagggagcc cttgcatgac aattctgcta acatcaaaag gcctctaggt
     6121 tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa caatacctgg gcccaccaca
     6181 ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt atacacccgc agagtactgc
     6241 aatttgactg tattaccaat gtcagcaaat tttctgtctt cgaagagtaa aaaattgtac
     6301 ttggcggata atgcctttag cggcttaact gtgccctcca tggaaaaatc agtcaagata
     6361 tccacatgtg tttttagtaa acaaattttg ggacctaatg cttcaactaa ctccagtaat
     6421 tccttggtgg tacgaacatc caatgaagca cacaagtttg tttgcttttc gtgcatgata
     6481 ttaaatagct tggcagcaac aggactagga tgagtagcag cacgttcctt atatgtagct
     6541 ttcgacatga tttatcttcg tttcctgcat gtttttgttc tgtgcagttg ggttaagaat
     6601 actgggcaat ttcatgtttc ttcaacacta catatgcgta tatataccaa tctaagtctg
     6661 tgctccttcc ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa atttcaagga
     6721 aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat tgaaaagcta attcttgaag
     6781 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     6841 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     6901 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     6961 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     7021 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     7081 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     7141 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     7201 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     7261 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     7321 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     7381 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     7441 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     7501 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     7561 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     7621 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     7681 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     7741 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     7801 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     7861 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     7921 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7981 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     8041 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     8101 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     8161 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     8221 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     8281 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     8341 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     8401 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     8461 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     8521 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     8581 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     8641 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     8701 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     8761 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     8821 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     8881 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     8941 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     9001 ccatgattac gccaagctta ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat
     9061 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     9121 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     9181 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     9241 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     9301 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     9361 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     9421 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     9481 gcgcgtccat tcgccaagct tcctgaaacg gagaaacata aacaggcatt gctgggatca
     9541 cccatacatc actctgtttt gcctgacctt ttccggtaat ttgaaaacaa acccggtctc
     9601 gaagcggaga tccggcgata attaccgcag aaataaaccc atacacgaga cgtagaacca
     9661 gccgcacatg gccggagaaa ctcctgcgag aatttcgtaa actcgcgcgc attgcatctg
     9721 tatttcctaa tgcggcactt ccaggcctcg atcgagaccg tttatccatt gcttttttgt
     9781 tgtctttttc cctcgttcac agaaagtctg aagaagctat agtagaacta tgagcttttt
     9841 ttgtttctgt tttccttttt tttttttt
//
