LOCUS       sequence-193784-       11288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MosSCI targeting vector expressing an ubiquitin ligase adaptor 
            ZIF-1, serving as a control for pOD2048-csnDEG. Expression is 
            controlled by Posm-6 (ciliated sensory neuron specific)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11288)
  AUTHORS   Wang S, Tang NH, Lara-Gonzalez P, Zhao Z, Cheerambathur DK, Prevo B,
            Chisholm AD, Desai A, Oegema K
  TITLE     A toolkit for GFP-mediated tissue-specific protein degradation in C.
            elegans.
  JOURNAL   Development. 2017 Jun 15. pii: dev.150094. doi: 10.1242/dev.150094.
  PUBMED    28619826
REFERENCE   2  (bases 1 to 11288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2017 Jun 15. pii: dev.150094. doi: 
            10.1242/dev.150094."
COMMENT     Sequence Label: pOD2049-csnControl
FEATURES             Location/Qualifiers
     source          1..11288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1491..1502)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      7112..7435
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     promoter        complement(7454..7472)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     9273..9295
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9333..9351)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9419..9523
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9524..9592,9593..10384)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9742..9761)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10555..11143
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11044..11063
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggccctgtc ggattatggg ctcttctttt atcctctact ttctttctag aaaatgcaaa
       61 tgttgagtca gaaattgctg aatggagtac aactgcaatg ggtttagctt atgtttctgg
      121 aacaattact ggtggaattg tgattgaaaa gtaaggttat atttcatttc aataaatgtt
      181 accacagtat aacaaaaaca agttacattg ttcagagtat gtctgaatgt attattatta
      241 ttgtatttgt gcatcagtac tgtagttgta ctgaagaagg gtttaaaata atctcaacgt
      301 ttttaagacg agttagtgag aatacggtcg cagttaggat gtttggacat agttttatta
      361 gataatcgtg aactaactca ctataaaaac ccgctaactc attgaataag atagttttaa
      421 acgttcaaaa tttgaagaca aaattgtatg aataagttta cagagttggt cgacgcaagc
      481 ttctccttct cttcacattt ctcaataatt tagctctaat tgcattcgta ttcttcgcaa
      541 aaatacgtat tctgattgac ccgatgaaat acggatgcct agttgcattg atcatctacg
      601 gttatactta tgggtgaggt ttttcaaata tattttgatt taatcgaata atgaaatttc
      661 agaactggag ttggtccaat ttcgtggttt atatcctctg aactggtacc tcaaaaacat
      721 cgtagtattg ctcaatctgt ggcatattca atgaatactg taatggtggt tatcagtaca
      781 tttacagtat tacctctata ttccgttatt ggtagttatg ctttccttat tctatattca
      841 attccttcat ttataagtat gcttatatta tttcgctact taccggaaac caaaggacga
      901 gagattcatg atattgtgaa tgaacttaaa aaaaaatgaa aacctttgaa aacgttcata
      961 cggccgaaat tttacttgat cgagccaatt gaacttttgt taagacttac agaaaagtga
     1021 gagattttgt tacaaaaaag tgagaaaaaa gcgaaaatga acaggaaaaa gaagaaaaat
     1081 acacccgaag tatcatataa aaattaaaaa gattgtttga cctggcggaa cttttttgca
     1141 aaaactctga atatccatgg cacatgcttc gtgcaaaaca ggatccaaat gaatatcaac
     1201 aagagattct tgaagacgac gagccacttg ctcagagcat tgcttatctc gaatgagacc
     1261 cttgttaaaa tcatttttca aacattatat gacattgtcg aaatgtcctc ctgattccat
     1321 gatggtagca aactcacttc gtctttgagc caatttatcc aagtccttgt aaaaagtatt
     1381 cgaaaattgt taacggataa aatgtttatt ataatatcaa aaacaatttg tcagttgacc
     1441 actttttgat atatttgaca gaaaccggga tgaattggct caaagttagg gcgcccttct
     1501 attacagggt ttctgataac aaaccggtta ttaactccca acaagggatg atttcaattc
     1561 atcatgctca aattgaccca aattaagtta catgacaaat tcatcgccct tttcaactct
     1621 tcttggctca tcatctgtta ttctgttcct attctgtctg caccctatac ccctttgcat
     1681 actctctcgt cattcctttc ttggatagtg tcttcttctc cccagctctg ctacttctat
     1741 gacttgcgcc cgctgctttt ccgctcgttc ctctcctctc tgacgtcttc ttctgcttct
     1801 tttctccttc ctttttccct tctcaatctt cctctttttc ccatttctgt caatcattcg
     1861 aagaagaaga agaagaagac cctcattcat ttcatttttt tttctgtcgg tgtgtgctgc
     1921 tcggttaaga gtgagctctc tattccacgt cttcttcttt ttcttcttcg attcgaatca
     1981 atcactccac caaaacgcat tcgtttttgg gattcacccc gcggttcgca ataggtttct
     2041 ttttcaaatt attagcgtta taaatagaaa aatggtggag tttcaataaa aatgataatt
     2101 tacaaagtgt attttgatta catgtactca aaaaggttga aatttttcat accagttttc
     2161 cggaaatcca tctgatatca ttatcgtatt tttcttttta aaaatgtttt caaaaaaaaa
     2221 caaaatatag ctggttattt ggcaccctct aattaccatt ttcctgtcac accacactct
     2281 tttccttctt ccacttcttt ccgttttcag ccgcttccaa ccaaaccgat atgaaagccg
     2341 agcaacaacg atcgattcca cccggctcgg cgaccttccc gtcgcaggtg agactcagaa
     2401 aactagagaa accgctcaac taactcttga tatccgattt cattcttttc tcttttcttt
     2461 ttgttgaact tttccatatt tccagatgcc acggccacca ccaagcaccg aacaaggaat
     2521 cacaacggaa tcggagcttg cgaagaaagc tcaaatcact ccgaacgacg ttttagcact
     2581 tccgggaatc actcaaggta tcttgctccc tttggatttc agtttaaaac ataaatttag
     2641 gattcttatg ctccccatct gcgaacatct ataacatcga gttcaccaag ttccaaatcc
     2701 gtgatctgga cactgagcaa gtgcttttcg agatcgccaa accggagaac gatcaggaga
     2761 atgatgagtc gccacaggag tcggcaagat acgtgcgtta tagatttgct ccaaactttt
     2821 tgaaactcaa aacggtcgga gcaactgtgg aattcaaagt aggagacatc ccaatccatc
     2881 atttccgaat gatcgaacgt cacttcttca aagatcgcct tctgaagtgt tttgactttg
     2941 aattcggatt ctgtattccg aattcacgaa acaactgtga acatatctat gagttccctc
     3001 aactctctca acaactcagt gagtcattat tctaaaaagt acaattcaaa agactaatct
     3061 ctttttcagt ggacgacatg atcaacaatc caaacgagac tcgttctgac agcttctatt
     3121 tcgtcgataa caaactcgtc atgcacaaca aagccgacta ctcatatgat gcataaatat
     3181 ttaatacaaa aatgttctgg ataattattc tgtcgaatag aaaaaaaact ccaaatgtga
     3241 ttaaattcca ataattcctg tctagtttgt tccttcccct tccccttctc atgttcaatg
     3301 cattcctaag cttttcagtt ccccccttgt ttctatattt tttcgctgtc ctgtcacact
     3361 cgctaaaaac actaatcaca cggaaatctg ttttcaataa aaactccaac tttaactcat
     3421 tttcaatttc aactgaaaga ttttttcatt agagaatgtc tagaactagg cccgggctac
     3481 gtaatacgac tcacttaagg ccttgactag agggtaccag agctcatttt ataattgctt
     3541 atatgtagta gttatatttt cagttttcat tacatttcat gggtatttat ttattaacta
     3601 taatcttgta taagacgatg taattatgaa acaacgattt cacacttccg gttttcatgt
     3661 aaaatttttt tcgttccaaa taaattgtta taaaattaat tacatctttc atcaaacttc
     3721 aaaaatgaaa ttgcattttt aataattagg agtctattac ggaattcatt aaatttcaga
     3781 aaacaaagtt aactatatat ttctctagta gttcctttcc caggagaccc ttccaagatt
     3841 tgtatccaca tgttaccata gtaaccactc attgcttctc gctcacattg tctgctccct
     3901 ctcttggggc ttatatctct ttcaagctat taccttcatt agtatacatc tatgagtgag
     3961 tgttccgcga gtaccagtca gttatctact ttctgtgtaa atgagatacc atggaagttg
     4021 gttccacagg taaaaatctt gaaaaccact cagtttcgac aaaacaaaaa tttcagactt
     4081 gcgacgaatt cagcgaatac gagttcatag atgaaaaata taggtgagga tctctagata
     4141 tatatctatt aaattttata accttttcag gcgcctttct gacgtagaat cgtttgtctg
     4201 gaagtggtcg ccgtgtggaa gatatggaat tgatcttccg ggcggtcgtg acgtcattaa
     4261 ccaacaacta cgtgaccgta tggctgtatt cgatctacat acgaacaaat ggaccctata
     4321 caccatggac cgtggtgctt tcgaaattcc acgagtttgc tttctctact gggcacgaaa
     4381 cgatataatc ggcacaataa cccttgtagt aagtgtccga tctaccatac ctaatccaaa
     4441 aaataaaatt tcagaaagac tatgaagcag gaccggatga tgaaattcca ttgaaattca
     4501 agcaatcatt cttctacgta tctcacgaaa cacagacatt agtgtactct ggttcatgca
     4561 agtaccgtac gaataaaaca atcgtagcat cttattggta ccatattttc gaaaatcgac
     4621 gtggcgaacc gttcattgat cacaataact acctgtggtt ggtattctca aacggagtgg
     4681 attctctgat tcttcttcca tttattccaa cttcaacgtg tttcaaagcg aaatgtgtat
     4741 catttgatat gaacgatatc gtcagtcaat tcactggtat tcgcgtattt ctcgatccat
     4801 ttgtatcatc tcactacgtg ccatgggtct acagaaatga cgttaatttt ttcattagga
     4861 ttgattcgca ttcatttatc gaggagcaaa accgggacta tggtctagat ccgcaaattg
     4921 ataacccgga gacgaaaccg cacagaggag tatttcaact tcgtgtgaat ttgaaagatg
     4981 tcatcgaaaa gggcgaaaaa gatgcgattc atgcagttaa tcctgaaaca atagagacaa
     5041 gattcaatcg aggtgacatt gaactatggc gaaaaatcag cgatgagctc cgtcaagaac
     5101 atcagagcat agcacagcat ggcaaaatgg ttgtagtaca acgatggtca cactttactg
     5161 ttgatatggg gctcagcaag aaaatgagaa ccgtaggata tgaaaatatt atttgttgat
     5221 agtttattaa tttaatttca gcgtgttgcc gatttggacg cccgtgatta ctgttgcagc
     5281 cataagccac gtaattcgtc attctgctgt attgacttgg acagcgagcg aatcaaggct
     5341 gtggatgctc tcattcttgg gtacaaatta atctattttt ttcctaagta tttttttttc
     5401 agaactgacg tggttcttcc ccatcctagt ggctctgtat tcctatttcg atacaaggaa
     5461 aattatggta atccctccac caaatagacg ttttcttcat cgtaattttt cagggatgag
     5521 ttattgcgag cttccattct tccagccact ttcattgaag ctaaaatgta ttcaagttct
     5581 caaggaatct gtcgaaatcg acccaagtta ctatagaatc aaatgctgcc ttaaaatgca
     5641 agaagaactt gaaaaagaaa ctgccgaaaa tttgtgcacg ataaatattc aacaataatg
     5701 gtctcttctc aataaaggtt gtatatttat tcatcttatt gaatctgcca tttcctcgtt
     5761 tttgcgagtt tatatacctt ccaattttct ttctattgta ttttcaactt ctaattttaa
     5821 ttcagggaaa ctgcttcaac gcatcatggg aggtagggcc ggctctgtct caaagggtga
     5881 agaagataac atggcaatta ttaaagagtt tatgcgtttc aaggtgcata tggagggatc
     5941 tgtcaatggg catgagtttg aaattgaagg tgaaggagaa ggccgaccat atgagggaac
     6001 acaaaccgca aaactaaagg taagtttaaa catatatata ctaactaacc ctgattattt
     6061 aaattttcag gtaactaaag gcggaccatt accattcgcc tgggacatcc tctctccaca
     6121 gttcatgtat ggaagtaaag cttatgttaa acatccggca gatataccag attatttgaa
     6181 actttcattc ccggagggtt ttaagtggga acgcgtaatg aattttgaag acggaggagt
     6241 tgttacagtg acgcaagact caaggtaagt ttaaacagtt cggtactaac taaccataca
     6301 tatttaaatt ttcagcctcc aagatggaga atttatttat aaagtcaaac ttcgaggaac
     6361 gaatttcccc tcggatggac ctgttatgca gaagaagact atgggatggg aagcttcaag
     6421 tgaaagaatg taccctgaag acggtgctct taagggagag attaaacaac gtcttaaatt
     6481 gaaagatgga ggacattacg atgctgaggt aagtttaaac atgattttac taactaacta
     6541 atctgattta aattttcagg tgaagacaac ttacaaagcc aaaaaaccag ttcagctgcc
     6601 aggagcgtac aatgttaata ttaaactgga tatcacctcc cacaacgagg attacactat
     6661 cgttgagcaa tatgaaagag ctgaagggcg gcactcgaca ggtggcatgg atgaattgta
     6721 taagggagga ggaggaggtg gaatgccacc aaagccatct gccaagggag ccaagaaggc
     6781 cgccaagacc gttacgaagc caaaggacgg aaagaagaga cgtcatgccc gtaaggaatc
     6841 atactccgtc tacatctacc gtgtcctcaa gcaagttcat ccagacactg gagtttcctc
     6901 caaagccatg tctatcatga actcttttgt caacgatgtc ttcgagcgta ttgctgctga
     6961 agcatcccgt cttgctcact acaacaagcg ttccacaatc tcatcccgcg aaattcagac
     7021 cgctgtccgt ctgatccttc caggagagct tgccaagcac gccgtgtctg agggaaccaa
     7081 ggccgttacc aagtacactt ccagcaagta aatgcaagat cctttcaagc attcccttct
     7141 tctctatcac tcttctttct ttttgtcaaa aaattctctc gctaatttat ttgctttttt
     7201 aatgttatta ttttatgact ttttatagtc actgaaaagt ttgcatctga gtgaagtgaa
     7261 tgctatcaaa atgtgattct gtctgatgta ctttcacaat ctctcttcaa ttccattttg
     7321 aagtgcttta aacccgaaag gttgagaaaa atgcgagcgc tcaaatattt gtattgtgtt
     7381 cgttgagtga cccaacaaaa agaggaaact ttattgtgcc gccaagaaaa aagtctcatt
     7441 gcagcttttg ttccctttag tgagggttaa ttcattcgaa gatctgccca ctagtgagtc
     7501 gtattataag tgcaagtaag atcagtgttt gtttcgatat cagtctgttt cgtaacggtc
     7561 ttctgtataa ctacaaaaaa gaataaaaaa ccgtatcaca actgagaaaa aagacaaaac
     7621 ttattgaaca gtgttccttt caaaaaatta ttttgtaaaa aatcgagttt ttctccaaaa
     7681 taacgcacgc aatcaaaaac aagaaaacta acgtgttctc ctgggcgtgg aactccttat
     7741 caagattacc attattgtct gtcagaattg atatttcaat tatttcatat ctagacaaac
     7801 ccgggtgtct tgcatttatt agtactgtga gtttacatac aaacgaggct tactcagata
     7861 acatagagtc acctctaatt agcaaaaacc catttctaat tagctatttc gaccagttct
     7921 aaagtcaccg taattagaaa tggtttttgt agcttttcta attagaaatg accccgagat
     7981 aagtgaaata tggcattttc tgggttttct agtaagaaat gatcccgcac gtagtgattc
     8041 cgcgttacaa gccaaattca aattaaaaaa gtatattaca caattcaagt tacattattt
     8101 tcaggaaaca atggcgaaga gcaaagcaga gattcaaaag gaggaaagag acaacagata
     8161 tgaacgaaga cttcttgaaa agagaaagag acatgaggtg agtaaccatg ataataaata
     8221 atcaagtaag ggctatagat ttataaattc tggagttaac agtttcgaaa aaaagtagtt
     8281 ttcatatgtg cgaaggaata tcaaaaatag gggaatagcc taaacgagtg agaaaattaa
     8341 atgtttgtta ttttttcaaa aatgtctgca gggccgttat cctttcaaat gcactctatc
     8401 aaacatagat catatccatc atatcttatc cagaatttat atcatatcca agattgagag
     8461 cgtttcaagg aaaatctcaa acaaccatac aattttgtag atcgacaaca aggcctccac
     8521 ttcttcatcg atggcctctg cagcacctgc accaccaagt ttgtattttt catattaata
     8581 taaagtgagc cacgcatagt cgtttttagg atttttgcca gatcgatgtc gcgcaaacag
     8641 actgtttttt tttctttgaa agccgtgcgt gacatgggcc acggattgta acgccgcgct
     8701 ggatggataa aaatttattc agttttttag cctttacaaa atatggttcg actactttct
     8761 ttatttttac atttttatag gtgctcattg agctgagata ttttgggtat aatcggaaaa
     8821 tcgctgattt tggtcatttt tctgcactaa agattacaca aaaagttcag tatttagatt
     8881 cattgttgaa aattggggat gatctacaga tacatatcgc cgtgttcaaa aacacacgtg
     8941 ctgtgacgtc gtgactgggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     9001 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     9061 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
     9121 cggtatttca caccgcatat ggtgcactct cagtgcaatc tgctctgatg ccgcatagtt
     9181 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     9241 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     9301 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     9361 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     9421 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     9481 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     9541 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     9601 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     9661 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     9721 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     9781 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     9841 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     9901 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     9961 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
    10021 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
    10081 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
    10141 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
    10201 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
    10261 actggggtca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
    10321 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
    10381 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
    10441 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
    10501 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
    10561 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
    10621 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
    10681 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
    10741 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
    10801 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
    10861 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
    10921 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
    10981 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
    11041 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    11101 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
    11161 ctttttacgg ttcctggtct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
    11221 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgatacca gcggataaca
    11281 atttcaca
//
