LOCUS       sequence-193863-        9658 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enhanced specificity ciCas9 (e-ciCas9) in mammalian cells.
            Can be used to generate Flp-In and Flp-In T-REx stables..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9658)
  AUTHORS   Rose JC, Stephany JJ, Valente WJ, Trevillian BM, Dang HV, Bielas JH,
            Maly DJ, Fowler DM
  TITLE     Rapidly inducible Cas9 and DSB-ddPCR to probe editing kinetics.
  JOURNAL   Nat Methods. 2017 Jul 24. doi: 10.1038/nmeth.4368.
  PUBMED    28737741
REFERENCE   2  (bases 1 to 9658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Jul 24. doi: 10.1038/nmeth.4368."
COMMENT     Sequence Label: e-ciCas9_pcDNA5
FEATURES             Location/Qualifiers
     source          1..9658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      972..981
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             981..1004
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5379..5399
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5610..5627)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5616..5840
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5973..5992)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6124..6171
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6179..7201
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6179..7201
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7331..7452
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7368..7387)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7422..7441
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7501..7517)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7501..7517)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7514..7536)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7525..7541
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7549..7555,7556..7573,7574..7579))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7594..7615
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7732..7749)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7903..8491)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7983..8002)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8662..9453,9454..9522))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRHIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9285..9304
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9523..9627)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt tgccaccatg gactacaagg acgacgacga caaggacaag aagtactcca
     1021 ttgggctcga tatcggcaca aacagcgtcg gctgggccgt cattacggac gagtacaagg
     1081 tgccgagcaa aaaattcaaa gttctgggca ataccgatcg ccacagcata aagaagaacc
     1141 tcattggcgc cctcctgttc gactccgggg agacggccga agccacgcgg ctcaaaagaa
     1201 cagcacggcg cagatatacc cgcagaaaga atcggatctg ctacctgcag gagatcttta
     1261 gtaatgagat ggctaaggtg gatgactctt tcttccatag gctggaggag tcctttttgg
     1321 tggaggagga taaaaagcac gagcgccacc caatctttgg caatatcgtg gacgaggtgg
     1381 cgtaccatga aaagtaccca accatatatc atctgaggaa gaagcttgta gacagtactg
     1441 ataaggctga cttgcggttg atctatctcg cgctggcgca tatgatcaaa tttcggggac
     1501 acttcctcat cgagggggac ctgaacccag acaacagcag caaccgggag ctggtggttg
     1561 actttctctc ctacaagctt tcccagaaag gatacagctg gagtcagttt agtgatgtgg
     1621 aagagaacag gactgaggcc ccagaaggga ctgaatcgga gatggagacc cccagtgcca
     1681 tcaatggcaa cccatcctgg cacctggcag acagccccgc ggtgaatgga gccactggcc
     1741 acagcagcag tttggatgcc cgggaggtga tccccatggc agcagtaaag caagcgctga
     1801 gggaggcagg cgacgagttt gaactgcggt accggcgggc attcagtgac ctgacatccc
     1861 agctccacat caccccaggg acagcatatc agagctttga acaggtagtg aatgaactct
     1921 tccgggatgg ggtaaactgg ggtcgcattg tggccttttt ctccttcggc ggggcactgt
     1981 gcgtggaaag cgtagacaag gagatgcagg tattggtgag tcggatcgca gcttggatgg
     2041 ccacttacct gaatgaccac ctagagcctt ggatccagga gaacggcggc tgggatactt
     2101 ttgtggaact ctatgggaac aatggtagcg gaactgcttc tgggactggg agtggcaccg
     2161 gttcagcaac aggaagcggc actgtgaaca cggagatcac caaagctccg ctgagcgcct
     2221 ctatgatcaa gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc
     2281 ggcagcagct gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg
     2341 ccggctacat tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc
     2401 tggaaaagat ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc
     2461 ggaagcagcg gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc
     2521 acgccattct gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga
     2581 tcgagaagat cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca
     2641 gcagattcgc ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg
     2701 aagtggtgga caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata
     2761 agaacctgcc caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg
     2821 tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc
     2881 tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga
     2941 ccgtgaagca gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa
     3001 tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa
     3061 ttatcaagga caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg
     3121 tgctgaccct gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg
     3181 cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg
     3241 gcaggctgag ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc
     3301 tggatttcct gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg
     3361 acagcctgac ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc
     3421 tgcacgagca cattgccaat ctggccggca gccccgccat taagaagggc atcctgcaga
     3481 cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg
     3541 tgatcgaaat ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga
     3601 gaatgaagcg gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc
     3661 ccgtggaaaa cacccagctg cagaacgaga agctgtacct gtactacctg cagaatgggc
     3721 gggatatgta cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacc
     3781 atatcgtgcc tcagagcttt ctggccgacg actccatcga caacaaggtg ctgaccagaa
     3841 gcgacaagaa ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga
     3901 agaactactg gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc
     3961 tgaccaaggc cgagagaggc ggcctgagcg aactggataa ggccggcttc atcaagagac
     4021 agctggtgga aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga
     4081 acactaagta cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt
     4141 ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca
     4201 actaccacca cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa
     4261 agtaccctgc gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga
     4321 agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca
     4381 gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagg
     4441 cgcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt
     4501 ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg
     4561 tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga
     4621 tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg
     4681 cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg
     4741 tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgaaaaa aaccccatcg
     4801 actttctcga ggcgaaagga tataaagagg tcaaaaaaga cctcatcatt aagcttccca
     4861 agtactctct ctttgagctt gaaaacggcc ggaaacgaat gctcgctagt gcgggcgagc
     4921 tgcagaaagg taacgagctg gcactgccct ctaaatacgt taatttcttg tatctggcca
     4981 gccactatga aaagctcaaa gggtctcccg aagataatga gcagaagcag ctgttcgtgg
     5041 aacaacacaa acactacctt gatgagatca tcgagcaaat aagcgaattc tccaaaagag
     5101 tgatcctcgc cgacgctaac ctcgataagg tgctttctgc ttacaataag cacagggata
     5161 agcccatcag ggagcaggca gaaaacatta tccacttgtt tactctgacc aacttgggcg
     5221 cgcctgcagc cttcaagtac ttcgacacca ccatagacag aaagcggtac acctctacaa
     5281 aggaggtcct ggacgccaca ctgattcatc agtcaattac ggggctctat gaaacaagaa
     5341 tcgacctctc tcagctcggt ggagacagca gggctgaccc caagaagaag aggaaggtga
     5401 caggaagcgg cactgctccc cccaatctct gggcagcgca gcgctacggc cgtgagctcc
     5461 gaaggatggc cgatgagggt gagggttcct tcaagtgaaa gcttggtacc gagctcggat
     5521 ccactagtcc agtgtggtgg aattctgcag atatccagca cagtggcggc cgctcgagtc
     5581 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     5641 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     5701 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     5761 gggtagggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     5821 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     5881 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     5941 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     6001 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     6061 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtac
     6121 ctagaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt ccttggccaa
     6181 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     6241 ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
     6301 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta
     6361 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga
     6421 attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga
     6481 cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat
     6541 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg
     6601 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg
     6661 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat
     6721 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa
     6781 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt
     6841 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat
     6901 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct
     6961 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
     7021 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg
     7081 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt
     7141 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata
     7201 gcacgtacta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     7261 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     7321 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     7381 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     7441 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     7501 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7561 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7621 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7681 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7741 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7801 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7861 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7921 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7981 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8041 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8101 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8161 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8221 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8281 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8341 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8401 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8461 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8521 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8581 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8641 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8701 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8761 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8821 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8881 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8941 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9001 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9061 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9121 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9181 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9241 gtgtatgtgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9301 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9361 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9421 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9481 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9541 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9601 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
