LOCUS       sequence-193880-       14838 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  First-generation rabies viral vector genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14838)
  AUTHORS   Chatterjee S, Sullivan HA, MacLennan BJ, Xu R, Hou Y, Lavin TK, Lea 
            NE, Michalski JE, Babcock KR, Dietrich S, Matthews GA, Beyeler A, 
            Calhoon GG, Glober G, Whitesell JD, Yao S, Cetin A, Harris JA, Zeng 
            H, Tye KM, Reid RC, Wickersham IR
  TITLE     Nontoxic, double-deletion-mutant rabies viral vectors for retrograde
            targeting of projection neurons.
  JOURNAL   Nat Neurosci. 2018 Apr;21(4):638-646. doi: 
            10.1038/s41593-018-0091-7. Epub 2018 Mar 5.
  PUBMED    29507411
REFERENCE   2  (bases 1 to 14838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-018-0091-7"; journalName: "Nat Neurosci"; date: 
            "2018-04"; volume: "21"; issue: "4"; pages: "638-646"
COMMENT     Sequence Label: pRVdG-4Cre
FEATURES             Location/Qualifiers
     source          1..14838
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..458
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(89..108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     299..320
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     584..606
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     598..615
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     599..615
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        657..1036
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1037..1240
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1191..1211
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1285..1304
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1285..1303
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4688..4697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4700..4720
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7501..7512
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12511..12529)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12525..12572
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(12595..12611)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12595..12611)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(12648..12668)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12648..12666)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12687..12703)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12687..12703)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12700..12722)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12711..12727
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12735..12741,12742..12759,12760..12765))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12780..12801
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12918..12935)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13089..13677)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13169..13188)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13848..14639,14640..14708))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14471..14490
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14709..14813)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaaat gagctgattt aacaaaaatt
      421 taacgcgaat tttaacaaaa tattaacgct tacaatttcc attcgccatt caggctgcgc
      481 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
      541 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
      601 aaaacgacgg ccagtgagcg cgcgataagc ttgatatcga attcctgcag cgcgttgaca
      661 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      721 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      781 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      841 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      901 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      961 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     1021 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     1081 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     1141 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     1201 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc
     1261 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta
     1321 gattaagcgt ctgatgagtc cgtgaggacg aaacccggcg taccgggtca cgcttaacaa
     1381 ccagatcaaa gaaaaaacag acattgtcaa ttgcaaagca aaaatgtaac acccctacaa
     1441 tggatgccga caagattgta ttcaaagtca ataatcaggt ggtctctttg aagcctgaga
     1501 ttatcgtgga tcaatatgag tacaagtacc ctgccatcaa agatttgaaa aagccctgta
     1561 taaccctagg aaaggctccc gatttaaata aagcatacaa gtcagttttg tcaggcatga
     1621 gcgccgccaa acttaatcct gacgatgtat gttcctattt ggcagcggca atgcagtttt
     1681 ttgaggggac atgtccggaa gactggacca gctatggaat tgtgattgca cgaaaaggag
     1741 ataagatcac cccaggttct ctggtggaga taaaacgtac tgatgtagaa gggaattggg
     1801 ctctgacagg aggcatggaa ctgacaagag accccactgt ccctgagcat gcgtccttag
     1861 tcggtcttct cttgagtctg tataggttga gcaaaatatc cgggcaaaac actggtaact
     1921 ataagacaaa cattgcagac aggatagagc agatttttga gacagcccct tttgttaaaa
     1981 tcgtggaaca ccatactcta atgacaactc acaaaatgtg tgctaattgg agtactatac
     2041 caaacttcag atttttggcc ggaacctatg acatgttttt ctcccggatt gagcatctat
     2101 attcagcaat cagagtgggc acagttgtca ctgcttatga agactgttca ggactggtat
     2161 catttactgg gttcataaaa caaatcaatc tcaccgctag agaggcaata ctatatttct
     2221 tccacaagaa ctttgaggaa gagataagaa gaatgtttga gccagggcag gagacagctg
     2281 ttcctcactc ttatttcatc cacttccgtt cactaggctt gagtgggaaa tctccttatt
     2341 catcaaatgc tgttggtcac gtgttcaatc tcattcactt tgtaggatgc tatatgggtc
     2401 aagtcagatc cctaaatgca acggttattg ctgcatgtgc tcctcatgaa atgtctgttc
     2461 tagggggcta tctgggagag gaattcttcg ggaaagggac atttgaaaga agattcttca
     2521 gagatgagaa agaacttcaa gaatacgagg cggctgaact gacaaagact gacgtagcac
     2581 tggcagatga tggaactgtc aactctgacg acgaggacta cttttcaggt gaaaccagaa
     2641 gtccggaggc tgtttatact cgaatcatga tgaatggagg tcgactaaag agatctcaca
     2701 tacggagata tgtctcagtc agttccaatc atcaagcccg tccaaactca ttcgccgagt
     2761 ttctaaacaa gacatattcg agtgactcat aagaagttga ataacaaaat gccggaaatc
     2821 tacggattgt gtatatccat catgaaaaaa actaacaccc ctcctttcga accatcccaa
     2881 acatgagcaa gatctttgtc aatcctagtg ctattagagc cggtctggcc gatcttgaga
     2941 tggctgaaga aactgttgat ctgatcaata gaaatatcga agacaatcag gctcatctcc
     3001 aaggggaacc catagaggtg gacaatctcc ctgaggatat ggggcgactt cacctggatg
     3061 atggaaaatc gcccaaccat ggtgagatag ccaaggtggg agaaggcaag tatcgagagg
     3121 actttcagat ggatgaagga gaggatccta gcttcctgtt ccagtcatac ctggaaaatg
     3181 ttggagtcca aatagtcaga caaatgaggt caggagagag atttctcaag atatggtcac
     3241 agaccgtaga agagattata tcctatgtcg cggtcaactt tcccaaccct ccaggaaagt
     3301 cttcagagga taaatcaacc cagactactg gccgagagct caagaaggag acaacaccca
     3361 ctccttctca gagagaaagc caatcatcga aagccaggat ggcggctcaa attgcttctg
     3421 gccctccagc ccttgaatgg tcggctacca atgaagagga tgatctatca gtggaggctg
     3481 agatcgctca ccagattgca gaaagtttct ccaaaaaata taagtttccc tctcgatcct
     3541 cagggatact cttgtataat tttgagcaat tgaaaatgaa ccttgatgat atagttaaag
     3601 aggcaaaaaa tgtaccaggt gtgacccgtt tagcccatga cgggtccaaa ctccccctaa
     3661 gatgtgtact gggatgggtc gctttggcca actctaagaa attccagttg ttagtcgaat
     3721 ccgacaagct gagtaaaatc atgcaagatg acttgaatcg ctatacatct tgctaaccga
     3781 acctctcccc tcagtccctc tagacaataa aatccgagat gtcccaaagt caacatgaaa
     3841 aaaacaggca acaccactga taaaatgaac ctcctacgta agatagtgaa aaaccgcagg
     3901 gacgaggaca ctcaaaaatc ctctcccgcg tcagcccctc tggatgacga tgacttgtgg
     3961 cttccacccc ctgaatacgt cccgctgaaa gaacttacag gcaagaagaa catgaggaac
     4021 ttttgtatca acggaagggt taaagtgtgt agcccgaatg gttactcgtt caggatcctg
     4081 cggcacattc tgaaatcatt cgacgagata tattctggga atcataggat gatcgggtta
     4141 gtcaaagtgg ttattggact ggctttgtca ggatctccag tccctgaggg cctgaactgg
     4201 gtatacaaat tgaggagaac ctttatcttc cagtgggctg attccagggg ccctcttgaa
     4261 ggggaggagt tggaatactc tcaggagatc acttgggatg atgatactga gttcgtcgga
     4321 ttgcaaataa gagtgattgc aaaacagtgt catatccagg gcagagtctg gtgtatcaac
     4381 atgaacccga gagcatgtca actatggtct gacatgtctc ttcagacaca aaggtccgaa
     4441 gaggacaaag attcctctct gcttctagaa taatcagatt atatcccgca aatttatcac
     4501 ttgtttacct ctggaggaga gaacatatgg gctcaactcc aacccttggg agcaatataa
     4561 caaaaaacat gttatggtgc cattaaaccg ctgcatttca tcaaagtcaa gttgattacc
     4621 tttacatttt gatcctcttg gatgtgaaaa aaactattaa catccctcaa aagaccccgg
     4681 gaaagccgcc accatggtgc ccaagaagaa gaggaaggtg agcaacctgc tgaccgtgca
     4741 ccagaacctg cccgccctgc ccgtggacgc caccagcgac gaggtgcgca agaacctgat
     4801 ggacatgttc cgcgaccgcc aggccttcag cgagcacacc tggaagatgc tgctgagcgt
     4861 gtgccgcagc tgggccgcct ggtgcaagct gaacaaccgc aagtggttcc ccgccgagcc
     4921 cgaggacgtg cgcgactacc tgctgtacct gcaggcccgc ggcctggccg tgaagaccat
     4981 ccagcagcac ctgggccagc tgaacatgct gcaccgccgc agcggcctgc cccgccccag
     5041 cgacagcaac gccgtgagcc tggtgatgcg ccgcatccgc aaggagaacg tggacgccgg
     5101 cgagcgcgcc aagcaggccc tggccttcga gcgcaccgac ttcgaccagg tgcgcagcct
     5161 gatggagaac agcgaccgct gccaggacat ccgcaacctg gccttcctgg gcatcgccta
     5221 caacaccctg ctgcgcatcg ccgagatcgc ccgcatccgc gtgaaggaca tcagccgcac
     5281 cgacggcggc cgcatgctga tccacatcgg ccgcaccaag accctggtga gcaccgccgg
     5341 cgtggagaag gccctgagcc tgggcgtgac caagctggtg gagcgctgga tcagcgtgag
     5401 cggcgtggcc gacgacccca acaactacct gttctgccgc gtgcgcaaga acggcgtggc
     5461 cgcccccagc gccaccagcc agctgagcac ccgggccctg gagggcatct tcgaggccac
     5521 ccaccgcctg atctacggcg ccaaggacga cagcggccag cgctacctgg cctggagcgg
     5581 ccacagcgcc cgcgtgggcg ccgcccgcga catggcccgc gccggcgtga gcatccccga
     5641 gatcatgcag gccggcggct ggaccaacgt gaacatcgtg atgaactaca tccgcaacct
     5701 ggacagcgag accggcgcca tggtgcgcct gctggaggac ggcgactgat gagctagctt
     5761 ataaagtgct gggtcatcta agcttttcag tcgagaaaaa aacattagat cagaagaaca
     5821 actggcaaca cttctcaacc tgagacttac ttcaagatgc tcgatcctgg agaggtctat
     5881 gatgacccta ttgacccaat cgagttagag gctgaaccca gaggaacccc cattgtcccc
     5941 aacatcttga ggaactctga ctacaatctc aactctcctt tgatagaaga tcctgctaga
     6001 ctaatgttag aatggttaaa aacagggaat agaccttatc ggatgactct aacagacaat
     6061 tgctccaggt ctttcagagt tttgaaagat tatttcaaga aggtagattt gggttctctc
     6121 aaggtgggcg gaatggctgc acagtcaatg atttctctct ggttatatgg tgcccactct
     6181 gaatccaaca ggagccggag atgtataaca gacttggccc atttctattc caagtcgtcc
     6241 cccatagaga agctgttgaa tctcacgcta ggaaatagag ggctgagaat ccccccagag
     6301 ggagtgttaa gttgccttga gagggttgat tatgataatg catttggaag gtatcttgcc
     6361 aacacgtatt cctcttactt gttcttccat gtaatcacct tatacatgaa cgccctagac
     6421 tgggatgaag aaaagaccat cctagcatta tggaaagatt taacctcagt ggacatcggg
     6481 aaggacttgg taaagttcaa agaccaaata tggggactgc tgatcgtgac aaaggacttt
     6541 gtttactccc aaagttccaa ttgtcttttt gacagaaact acacacttat gctaaaagat
     6601 cttttcttgt ctcgcttcaa ctccttaatg gtcttgctct ctcccccaga gccccgatac
     6661 tcagatgact tgatatctca actatgccag ctgtacattg ctggggatca agtcttgtct
     6721 atgtgtggaa actccggcta tgaagtcatc aaaatattgg agccatatgt cgtgaatagt
     6781 ttagtccaga gagcagaaaa gtttaggcct ctcattcatt ccttgggaga ctttcctgta
     6841 tttataaaag acaaggtaag tcaacttgaa gagacgttcg gtccctgtgc aagaaggttc
     6901 tttagggctc tggatcaatt cgacaacata catgacttgg tttttgtgtt tggctgttac
     6961 aggcattggg ggcacccata tatagattat cgaaagggtc tgtcaaaact atatgatcag
     7021 gttcacctta aaaaaatgat agataagtcc taccaggagt gcttagcaag cgacctagcc
     7081 aggaggatcc ttagatgggg ttttgataag tactccaagt ggtatctgga ttcaagattc
     7141 ctagcccgag accacccctt gactccttat atcaaaaccc aaacatggcc acccaaacat
     7201 attgtagact tggtggggga tacatggcac aagctcccga tcacgcagat ctttgagatt
     7261 cctgaatcaa tggatccgtc agaaatattg gatgacaaat cacattcttt caccagaacg
     7321 agactagctt cttggctgtc agaaaaccga ggggggcctg ttcctagcga aaaagttatt
     7381 atcacggccc tgtctaagcc gcctgtcaat ccccgagagt ttctgaggtc tatagacctc
     7441 ggaggattgc cagatgaaga cttgataatt ggcctcaagc caaaggaacg ggaattgaag
     7501 attgaaggtc gattctttgc tctaatgtca tggaatctaa gattgtattt tgtcatcact
     7561 gaaaaactct tggccaacta catcttgcca ctttttgacg cgctgactat gacagacaac
     7621 ctgaacaagg tgtttaaaaa gctgatcgac agggtcaccg ggcaagggct tttggactat
     7681 tcaagggtca catatgcatt tcacctggac tatgaaaagt ggaacaacca tcaaagatta
     7741 gagtcaacag aggatgtatt ttctgtccta gatcaagtgt ttggattgaa gagagtgttt
     7801 tctagaacac acgagttttt tcaaaaggcc tggatctatt attcagacag atcagacctc
     7861 atcgggttac gggaggatca aatatactgc ttagatgcgt ccaacggccc aacctgttgg
     7921 aatggccagg atggcgggct agaaggctta cggcagaagg gctggagtct agtcagctta
     7981 ttgatgatag atagagaatc tcaaatcagg aacacaagaa ccaaaatact agctcaagga
     8041 gacaaccagg ttttatgtcc gacatacatg ttgtcgccag ggctatctca agaggggctc
     8101 ctctatgaat tggagagaat atcaaggaat gcactttcga tatacagagc cgtcgaggaa
     8161 ggggcatcta agctagggct gatcatcaag aaagaagaga ccatgtgtag ttatgacttc
     8221 ctcatctatg gaaaaacccc tttgtttaga ggtaacatat tggtgcctga gtccaaaaga
     8281 tgggccagag tctcttgcgt ctctaatgac caaatagtca acctcgccaa tataatgtcg
     8341 acagtgtcca ccaatgcgct aacagtggca caacactctc aatctttgat caaaccgatg
     8401 agggattttc tgctcatgtc agtacaggca gtctttcact acctgctatt tagcccaatc
     8461 ttaaagggaa gagtttacaa gattctgagc gctgaagggg agagctttct cctagccatg
     8521 tcaaggataa tctatctaga tccttctttg ggagggatat ctggaatgtc cctcggaaga
     8581 ttccatatac gacagttctc agaccctgtc tctgaagggt tatccttctg gagagagatc
     8641 tggttaagct cccaagagtc ctggattcac gcgttgtgtc aagaggctgg aaacccagat
     8701 cttggagaga gaacactcga gagcttcact cgccttctag aagatccgac caccttaaat
     8761 atcagaggag gggccagtcc taccattcta ctcaaggatg caatcagaaa ggctttatat
     8821 gacgaggtgg acaaggtgga aaattcagag tttcgagagg caatcctgtt gtccaagacc
     8881 catagagata attttatact cttcttaata tctgttgagc ctctgtttcc tcgatttctc
     8941 agtgagctat tcagttcgtc ttttttggga atccccgagt caatcattgg attgatacaa
     9001 aactcccgaa cgataagaag gcagtttaga aagagtctct caaaaacttt agaagaatcc
     9061 ttctacaact cagagatcca cgggattagt cggatgaccc agacacctca gagggttggg
     9121 ggggtgtggc cttgctcttc agagagggca gatctactta gggagatctc ttggggaaga
     9181 aaagtggtag gcacgacagt tcctcaccct tctgagatgt tgggattact tcccaagtcc
     9241 tctatttctt gcacttgtgg agcaacagga ggaggcaatc ctagagtttc tgtatcagta
     9301 ctcccgtcct ttgatcagtc atttttttca cgaggccccc taaagggata cttgggctcg
     9361 tccacctcta tgtcgaccca gctattccat gcatgggaaa aagtcactaa tgttcatgtg
     9421 gtgaagagag ctctatcgtt aaaagaatct ataaactggt tcattactag agattccaac
     9481 ttggctcaag ctctaattag gaacattatg tctctgacag gccctgattt ccctctagag
     9541 gaggcccctg tcttcaaaag gacggggtca gccttgcata ggttcaagtc tgccagatac
     9601 agcgaaggag ggtattcttc tgtctgcccg aacctcctct ctcatatttc tgttagtaca
     9661 gacaccatgt ctgatttgac ccaagacggg aagaactacg atttcatgtt ccagccattg
     9721 atgctttatg cacagacatg gacatcagag ctggtacaga gagacacaag gctaagagac
     9781 tctacgtttc attggcacct ccgatgcaac aggtgtgtga gacccattga cgacgtgacc
     9841 ctggagacct ctcagatctt cgagtttccg gatgtgtcga aaagaatatc cagaatggtt
     9901 tctggggctg tgcctcactt ccagaggctt cccgatatcc gtctgagacc aggagatttt
     9961 gaatctctaa gcggtagaga aaagtctcac catatcggat cagctcaggg gctcttatac
    10021 tcaatcttag tggcaattca cgactcagga tacaatgatg gaaccatctt ccctgtcaac
    10081 atatacggca aggtttcccc tagagactat ttgagagggc tcgcaagggg agtattgata
    10141 ggatcctcga tttgcttctt gacaagaatg acaaatatca atattaatag acctcttgaa
    10201 ttggtctcag gggtaatctc atatattctc ctgaggctag ataaccatcc ctccttgtac
    10261 ataatgctca gagaaccgtc tcttagagga gagatatttt ctatccctca gaaaatcccc
    10321 gccgcttatc caaccactat gaaagaaggc aacagatcaa tcttgtgtta tctccaacat
    10381 gtgctacgct atgagcgaga gataatcacg gcgtctccag agaatgactg gctatggatc
    10441 ttttcagact ttagaagtgc caaaatgacg tacctatccc tcattactta ccagtctcat
    10501 cttctactcc agagggttga gagaaaccta tctaagagta tgagagataa cctgcgacaa
    10561 ttgagttctt tgatgaggca ggtgctgggc gggcacggag aagatacctt agagtcagac
    10621 gacaacattc aacgactgct aaaagactct ttacgaagga caagatgggt ggatcaagag
    10681 gtgcgccatg cagctagaac catgactgga gattacagcc ccaacaagaa ggtgtcccgt
    10741 aaggtaggat gttcagaatg ggtctgctct gctcaacagg ttgcagtctc tacctcagca
    10801 aacccggccc ctgtctcgga gcttgacata agggccctct ctaagaggtt ccagaaccct
    10861 ttgatctcgg gcttgagagt ggttcagtgg gcaaccggtg ctcattataa gcttaagcct
    10921 attctagatg atctcaatgt tttcccatct ctctgccttg tagttgggga cgggtcaggg
    10981 gggatatcaa gggcagtcct caacatgttt ccagatgcca agcttgtgtt caacagtctt
    11041 ttagaggtga atgacctgat ggcttccgga acacatccac tgcctccttc agcaatcatg
    11101 aggggaggaa atgatatcgt ctccagagtg atagatcttg actcaatctg ggaaaaaccg
    11161 tccgacttga gaaacttggc aacctggaaa tacttccagt cagtccaaaa gcaggtcaac
    11221 atgtcctatg acctcattat ttgcgatgca gaagttactg acattgcatc tatcaaccgg
    11281 atcaccctgt taatgtccga ttttgcattg tctatagatg gaccactcta tttggtcttc
    11341 aaaacttatg ggactatgct agtaaatcca aactacaagg ctattcaaca cctgtcaaga
    11401 gcgttcccct cggtcacagg gtttatcacc caagtaactt cgtctttttc atctgagctc
    11461 tacctccgat tctccaaacg agggaagttt ttcagagatg ctgagtactt gacctcttcc
    11521 acccttcgag aaatgagcct tgtgttattc aattgtagca gccccaagag tgagatgcag
    11581 agagctcgtt ccttgaacta tcaggatctt gtgagaggat ttcctgaaga aatcatatca
    11641 aatccttaca atgagatgat cataactctg attgacagtg atgtagaatc ttttctagtc
    11701 cacaagatgg ttgatgatct tgagttacag aggggaactc tgtctaaagt ggctatcatt
    11761 atagccatca tgatagtttt ctccaacaga gtcttcaacg tttccaaacc cctaactgac
    11821 ccctcgttct atccaccgtc tgatcccaaa atcctgaggc acttcaacat atgttgcagt
    11881 actatgatgt atctatctac tgctttaggt gacgtcccta gcttcgcaag acttcacgac
    11941 ctgtataaca gacctataac ttattacttc agaaagcaag tcattcgagg gaacgtttat
    12001 ctatcttgga gttggtccaa cgacacctca gtgttcaaaa gggtagcctg taattctagc
    12061 ctgagtctgt catctcactg gatcaggttg atttacaaga tagtgaagac taccagactc
    12121 gttggcagca tcaaggatct atccagagaa gtggaaagac accttcatag gtacaacagg
    12181 tggatcaccc tagaggatat cagatctaga tcatccctac tagactacag ttgcctgtga
    12241 accggatact cctggaagcc tgcccatgct aagactcttg tgtgatgtat cttgaaaaaa
    12301 acaagatcct aaatctgaac ctttggttgt ttgattgttt ttctcatttt tgttgtttat
    12361 ttgttaagcg tgggtcggca tggcatctcc acctcctcgc ggtccgacct gggcatccga
    12421 aggaggacgc acgtccactc ggatggctaa gggagggcgg ggatccggct gctaacaaag
    12481 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
    12541 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatcca
    12601 ctagttctag agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg
    12661 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
    12721 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
    12781 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
    12841 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
    12901 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    12961 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    13021 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    13081 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    13141 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    13201 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    13261 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    13321 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    13381 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    13441 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    13501 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
    13561 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    13621 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    13681 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    13741 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    13801 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    13861 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    13921 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    13981 ataccgcgag acccaccctt ggaggctcca gatttatcag caataaacca gccagccgga
    14041 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    14101 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    14161 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    14221 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    14281 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    14341 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    14401 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    14461 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    14521 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    14581 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    14641 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    14701 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    14761 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    14821 ccccgaaaag tgccacct
//
