LOCUS       sequence-193882-        8350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Second-generation rabies viral vector genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Chatterjee S, Sullivan HA, MacLennan BJ, Xu R, Hou Y, Lavin TK, Lea 
            NE, Michalski JE, Babcock KR, Dietrich S, Matthews GA, Beyeler A, 
            Calhoon GG, Glober G, Whitesell JD, Yao S, Cetin A, Harris JA, Zeng 
            H, Tye KM, Reid RC, Wickersham IR
  TITLE     Nontoxic, double-deletion-mutant rabies viral vectors for retrograde
            targeting of projection neurons.
  JOURNAL   Nat Neurosci. 2018 Apr;21(4):638-646. doi: 
            10.1038/s41593-018-0091-7. Epub 2018 Mar 5.
  PUBMED    29507411
REFERENCE   2  (bases 1 to 8350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-018-0091-7"; journalName: "Nat Neurosci"; date: 
            "2018-04"; volume: "21"; issue: "4"; pages: "638-646"
COMMENT     Sequence Label: pRVdGL-4Cre
FEATURES             Location/Qualifiers
     source          1..8350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        59..438
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        439..642
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     593..613
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     687..706
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        687..705
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4090..4099
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4102..4122
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5425..5443)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5439..5486
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(5509..5525)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5509..5525)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5562..5582)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5562..5580)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5601..5617)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5601..5617)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5614..5636)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5625..5641
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5649..5655,5656..5673,5674..5679))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5694..5715
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5832..5849)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6003..6591)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6083..6102)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6762..7553,7554..7622))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7385..7404
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7623..7727)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7754..8210
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7841..7860)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8051..8072
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     join(8336..8350,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(8350,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgag cgcgcgataa gcttgatatc gaattcctgc agcgcgttga
       61 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
      121 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      181 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      241 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      301 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      361 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      421 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      481 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      541 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      601 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa
      661 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagctggc
      721 tagattaagc gtctgatgag tccgtgagga cgaaacccgg cgtaccgggt cacgcttaac
      781 aaccagatca aagaaaaaac agacattgtc aattgcaaag caaaaatgta acacccctac
      841 aatggatgcc gacaagattg tattcaaagt caataatcag gtggtctctt tgaagcctga
      901 gattatcgtg gatcaatatg agtacaagta ccctgccatc aaagatttga aaaagccctg
      961 tataacccta ggaaaggctc ccgatttaaa taaagcatac aagtcagttt tgtcaggcat
     1021 gagcgccgcc aaacttaatc ctgacgatgt atgttcctat ttggcagcgg caatgcagtt
     1081 ttttgagggg acatgtccgg aagactggac cagctatgga attgtgattg cacgaaaagg
     1141 agataagatc accccaggtt ctctggtgga gataaaacgt actgatgtag aagggaattg
     1201 ggctctgaca ggaggcatgg aactgacaag agaccccact gtccctgagc atgcgtcctt
     1261 agtcggtctt ctcttgagtc tgtataggtt gagcaaaata tccgggcaaa acactggtaa
     1321 ctataagaca aacattgcag acaggataga gcagattttt gagacagccc cttttgttaa
     1381 aatcgtggaa caccatactc taatgacaac tcacaaaatg tgtgctaatt ggagtactat
     1441 accaaacttc agatttttgg ccggaaccta tgacatgttt ttctcccgga ttgagcatct
     1501 atattcagca atcagagtgg gcacagttgt cactgcttat gaagactgtt caggactggt
     1561 atcatttact gggttcataa aacaaatcaa tctcaccgct agagaggcaa tactatattt
     1621 cttccacaag aactttgagg aagagataag aagaatgttt gagccagggc aggagacagc
     1681 tgttcctcac tcttatttca tccacttccg ttcactaggc ttgagtggga aatctcctta
     1741 ttcatcaaat gctgttggtc acgtgttcaa tctcattcac tttgtaggat gctatatggg
     1801 tcaagtcaga tccctaaatg caacggttat tgctgcatgt gctcctcatg aaatgtctgt
     1861 tctagggggc tatctgggag aggaattctt cgggaaaggg acatttgaaa gaagattctt
     1921 cagagatgag aaagaacttc aagaatacga ggcggctgaa ctgacaaaga ctgacgtagc
     1981 actggcagat gatggaactg tcaactctga cgacgaggac tacttttcag gtgaaaccag
     2041 aagtccggag gctgtttata ctcgaatcat gatgaatgga ggtcgactaa agagatctca
     2101 catacggaga tatgtctcag tcagttccaa tcatcaagcc cgtccaaact cattcgccga
     2161 gtttctaaac aagacatatt cgagtgactc ataagaagtt gaataacaaa atgccggaaa
     2221 tctacggatt gtgtatatcc atcatgaaaa aaactaacac ccctcctttc gaaccatccc
     2281 aaacatgagc aagatctttg tcaatcctag tgctattaga gccggtctgg ccgatcttga
     2341 gatggctgaa gaaactgttg atctgatcaa tagaaatatc gaagacaatc aggctcatct
     2401 ccaaggggaa cccatagagg tggacaatct ccctgaggat atggggcgac ttcacctgga
     2461 tgatggaaaa tcgcccaacc atggtgagat agccaaggtg ggagaaggca agtatcgaga
     2521 ggactttcag atggatgaag gagaggatcc tagcttcctg ttccagtcat acctggaaaa
     2581 tgttggagtc caaatagtca gacaaatgag gtcaggagag agatttctca agatatggtc
     2641 acagaccgta gaagagatta tatcctatgt cgcggtcaac tttcccaacc ctccaggaaa
     2701 gtcttcagag gataaatcaa cccagactac tggccgagag ctcaagaagg agacaacacc
     2761 cactccttct cagagagaaa gccaatcatc gaaagccagg atggcggctc aaattgcttc
     2821 tggccctcca gcccttgaat ggtcggctac caatgaagag gatgatctat cagtggaggc
     2881 tgagatcgct caccagattg cagaaagttt ctccaaaaaa tataagtttc cctctcgatc
     2941 ctcagggata ctcttgtata attttgagca attgaaaatg aaccttgatg atatagttaa
     3001 agaggcaaaa aatgtaccag gtgtgacccg tttagcccat gacgggtcca aactccccct
     3061 aagatgtgta ctgggatggg tcgctttggc caactctaag aaattccagt tgttagtcga
     3121 atccgacaag ctgagtaaaa tcatgcaaga tgacttgaat cgctatacat cttgctaacc
     3181 gaacctctcc cctcagtccc tctagacaat aaaatccgag atgtcccaaa gtcaacatga
     3241 aaaaaacagg caacaccact gataaaatga acctcctacg taagatagtg aaaaaccgca
     3301 gggacgagga cactcaaaaa tcctctcccg cgtcagcccc tctggatgac gatgacttgt
     3361 ggcttccacc ccctgaatac gtcccgctga aagaacttac aggcaagaag aacatgagga
     3421 acttttgtat caacggaagg gttaaagtgt gtagcccgaa tggttactcg ttcaggatcc
     3481 tgcggcacat tctgaaatca ttcgacgaga tatattctgg gaatcatagg atgatcgggt
     3541 tagtcaaagt ggttattgga ctggctttgt caggatctcc agtccctgag ggcctgaact
     3601 gggtatacaa attgaggaga acctttatct tccagtgggc tgattccagg ggccctcttg
     3661 aaggggagga gttggaatac tctcaggaga tcacttggga tgatgatact gagttcgtcg
     3721 gattgcaaat aagagtgatt gcaaaacagt gtcatatcca gggcagagtc tggtgtatca
     3781 acatgaaccc gagagcatgt caactatggt ctgacatgtc tcttcagaca caaaggtccg
     3841 aagaggacaa agattcctct ctgcttctag aataatcaga ttatatcccg caaatttatc
     3901 acttgtttac ctctggagga gagaacatat gggctcaact ccaacccttg ggagcaatat
     3961 aacaaaaaac atgttatggt gccattaaac cgctgcattt catcaaagtc aagttgatta
     4021 cctttacatt ttgatcctct tggatgtgaa aaaaactatt aacatccctc aaaagacccc
     4081 gggaaagccg ccaccatggt gcccaagaag aagaggaagg tgagcaacct gctgaccgtg
     4141 caccagaacc tgcccgccct gcccgtggac gccaccagcg acgaggtgcg caagaacctg
     4201 atggacatgt tccgcgaccg ccaggccttc agcgagcaca cctggaagat gctgctgagc
     4261 gtgtgccgca gctgggccgc ctggtgcaag ctgaacaacc gcaagtggtt ccccgccgag
     4321 cccgaggacg tgcgcgacta cctgctgtac ctgcaggccc gcggcctggc cgtgaagacc
     4381 atccagcagc acctgggcca gctgaacatg ctgcaccgcc gcagcggcct gccccgcccc
     4441 agcgacagca acgccgtgag cctggtgatg cgccgcatcc gcaaggagaa cgtggacgcc
     4501 ggcgagcgcg ccaagcaggc cctggccttc gagcgcaccg acttcgacca ggtgcgcagc
     4561 ctgatggaga acagcgaccg ctgccaggac atccgcaacc tggccttcct gggcatcgcc
     4621 tacaacaccc tgctgcgcat cgccgagatc gcccgcatcc gcgtgaagga catcagccgc
     4681 accgacggcg gccgcatgct gatccacatc ggccgcacca agaccctggt gagcaccgcc
     4741 ggcgtggaga aggccctgag cctgggcgtg accaagctgg tggagcgctg gatcagcgtg
     4801 agcggcgtgg ccgacgaccc caacaactac ctgttctgcc gcgtgcgcaa gaacggcgtg
     4861 gccgccccca gcgccaccag ccagctgagc acccgggccc tggagggcat cttcgaggcc
     4921 acccaccgcc tgatctacgg cgccaaggac gacagcggcc agcgctacct ggcctggagc
     4981 ggccacagcg cccgcgtggg cgccgcccgc gacatggccc gcgccggcgt gagcatcccc
     5041 gagatcatgc aggccggcgg ctggaccaac gtgaacatcg tgatgaacta catccgcaac
     5101 ctggacagcg agaccggcgc catggtgcgc ctgctggagg acggcgactg atgaaccgga
     5161 tactcctgga agcctgccca tgctaagact cttgtgtgat gtatcttgaa aaaaacaaga
     5221 tcctaaatct gaacctttgg ttgtttgatt gtttttctca tttttgttgt ttatttgtta
     5281 agcgtgggtc ggcatggcat ctccacctcc tcgcggtccg acctgggcat ccgaaggagg
     5341 acgcacgtcc actcggatgg ctaagggagg gcggggatcc ggctgctaac aaagcccgaa
     5401 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
     5461 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tccactagtt
     5521 ctagagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt
     5581 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     5641 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     5701 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     5761 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     5821 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     5881 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     5941 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6001 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6061 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6121 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6181 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6241 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6301 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6361 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6421 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     6481 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     6541 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     6601 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     6661 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     6721 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     6781 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     6841 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     6901 cgagacccac ccttggaggc tccagattta tcagcaataa accagccagc cggaagggcc
     6961 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7021 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7081 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7141 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7201 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     7261 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     7321 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     7381 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     7441 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     7501 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     7561 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     7621 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     7681 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     7741 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     7801 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     7861 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     7921 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     7981 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     8041 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     8101 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa atgagctgat
     8161 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt ccattcgcca
     8221 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     8281 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     8341 tcacgacgtt
//
