LOCUS       sequence-193884-        8244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Second-generation rabies viral vector genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8244)
  AUTHORS   Chatterjee S, Sullivan HA, MacLennan BJ, Xu R, Hou Y, Lavin TK, Lea 
            NE, Michalski JE, Babcock KR, Dietrich S, Matthews GA, Beyeler A, 
            Calhoon GG, Glober G, Whitesell JD, Yao S, Cetin A, Harris JA, Zeng 
            H, Tye KM, Reid RC, Wickersham IR
  TITLE     Nontoxic, double-deletion-mutant rabies viral vectors for retrograde
            targeting of projection neurons.
  JOURNAL   Nat Neurosci. 2018 Apr;21(4):638-646. doi: 
            10.1038/s41593-018-0091-7. Epub 2018 Mar 5.
  PUBMED    29507411
REFERENCE   2  (bases 1 to 8244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-018-0091-7"; journalName: "Nat Neurosci"; date: 
            "2018-04"; volume: "21"; issue: "4"; pages: "638-646"
COMMENT     Sequence Label: pRVdGL-1Cre
FEATURES             Location/Qualifiers
     source          1..8244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        59..438
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        439..642
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     593..613
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     687..706
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        687..705
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      854..863
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             866..886
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5319..5337)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5333..5380
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(5403..5419)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5403..5419)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5456..5476)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5456..5474)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5495..5511)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5495..5511)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5508..5530)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5519..5535
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5543..5549,5550..5567,5568..5573))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5588..5609
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5726..5743)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5897..6485)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5977..5996)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6656..7447,7448..7516))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7279..7298
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7517..7621)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7648..8104
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7735..7754)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7945..7966
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     join(8230..8244,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(8244,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgag cgcgcgataa gcttgatatc gaattcctgc agcgcgttga
       61 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
      121 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      181 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      241 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      301 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      361 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      421 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      481 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      541 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      601 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa
      661 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagctggc
      721 tagattaagc gtctgatgag tccgtgagga cgaaacccgg cgtaccgggt cacgcttaac
      781 aaccagatca aagaaaaaac agacattgtc aattgcaaag caaaaatgta acacccctac
      841 accggtgaaa gccgccacca tggtgcccaa gaagaagagg aaggtgagca acctgctgac
      901 cgtgcaccag aacctgcccg ccctgcccgt ggacgccacc agcgacgagg tgcgcaagaa
      961 cctgatggac atgttccgcg accgccaggc cttcagcgag cacacctgga agatgctgct
     1021 gagcgtgtgc cgcagctggg ccgcctggtg caagctgaac aaccgcaagt ggttccccgc
     1081 cgagcccgag gacgtgcgcg actacctgct gtacctgcag gcccgcggcc tggccgtgaa
     1141 gaccatccag cagcacctgg gccagctgaa catgctgcac cgccgcagcg gcctgccccg
     1201 ccccagcgac agcaacgccg tgagcctggt gatgcgccgc atccgcaagg agaacgtgga
     1261 cgccggcgag cgcgccaagc aggccctggc cttcgagcgc accgacttcg accaggtgcg
     1321 cagcctgatg gagaacagcg accgctgcca ggacatccgc aacctggcct tcctgggcat
     1381 cgcctacaac accctgctgc gcatcgccga gatcgcccgc atccgcgtga aggacatcag
     1441 ccgcaccgac ggcggccgca tgctgatcca catcggccgc accaagaccc tggtgagcac
     1501 cgccggcgtg gagaaggccc tgagcctggg cgtgaccaag ctggtggagc gctggatcag
     1561 cgtgagcggc gtggccgacg accccaacaa ctacctgttc tgccgcgtgc gcaagaacgg
     1621 cgtggccgcc cccagcgcca ccagccagct gagcacccgg gccctggagg gcatcttcga
     1681 ggccacccac cgcctgatct acggcgccaa ggacgacagc ggccagcgct acctggcctg
     1741 gagcggccac agcgcccgcg tgggcgccgc ccgcgacatg gcccgcgccg gcgtgagcat
     1801 ccccgagatc atgcaggccg gcggctggac caacgtgaac atcgtgatga actacatccg
     1861 caacctggac agcgagaccg gcgccatggt gcgcctgctg gaggacggcg actgatgacc
     1921 tagggaagtt gaataacaaa atgccggaaa tctacggatt gtgtatatcc atcatgaaaa
     1981 aaactaacac ccctcctttc gaaccatccc aaacatggat gccgacaaga ttgtattcaa
     2041 agtcaataat caggtggtct ctttgaagcc tgagattatc gtggatcaat atgagtacaa
     2101 gtaccctgcc atcaaagatt tgaaaaagcc ctgtataact ctaggaaagg ctcccgattt
     2161 aaataaagca tacaagtcag ttttgtcagg catgagcgcc gccaaactta atcctgacga
     2221 tgtatgttcc tatttggcag cggcaatgca gttttttgag gggacatgtc cggaagactg
     2281 gaccagctat ggaattgtga ttgcacgaaa aggagataag atcaccccag gttctctggt
     2341 ggagataaaa cgtactgatg tagaagggaa ttgggctctg acaggaggca tggaactgac
     2401 aagagacccc actgtccctg agcatgcgtc cttagtcggt cttctcttga gtctgtatag
     2461 gttgagcaaa atatccgggc aaaacactgg taactataag acaaacattg cagacaggat
     2521 agagcagatt tttgagacag ccccttttgt taaaatcgtg gaacaccata ctctaatgac
     2581 aactcacaaa atgtgtgcta attggagtac tataccaaac ttcagatttt tggccggaac
     2641 ctatgacatg tttttctccc ggattgagca tctatattca gcaatcagag tgggcacagt
     2701 tgtcactgct tatgaagact gttcaggact ggtatcattt actgggttca taaaacaaat
     2761 caatctcacc gctagagagg caatactata tttcttccac aagaactttg aggaagagat
     2821 aagaagaatg tttgagccag ggcaggagac agctgttcct cactcttatt tcatccactt
     2881 ccgttcacta ggcttgagtg ggaaatctcc ttattcatca aatgctgttg gtcacgtgtt
     2941 caatctcatt cactttgtag gatgctatat gggtcaagtc agatccctaa atgcaacggt
     3001 tattgctgca tgtgctcctc atgaaatgtc tgttctaggg ggctatctgg gagaggaatt
     3061 cttcgggaaa gggacatttg aaagaagatt cttcagagat gagaaagaac ttcaagaata
     3121 cgaggcggct gaactgacaa agactgacgt agcactggca gatgatggaa ctgtcaactc
     3181 tgacgacgag gactactttt caggtgaaac cagaagtccg gaggctgttt atactcgaat
     3241 catgatgaat ggaggtcgac taaagagatc tcacatacgg agatatgtct cagtcagttc
     3301 caatcatcaa gcccgtccaa actcattcgc cgagtttcta aacaagacat attcgagtga
     3361 ctcataagaa gttgaataac aaaatgccgg aaatctacgg attgtgtata tccatcatga
     3421 aaaaaactaa cacccctcct ttcgaaccat cccaaacatg agcaagatct ttgtcaatcc
     3481 tagtgctatt agagccggtc tggccgatct tgagatggct gaagaaactg ttgatctgat
     3541 caatagaaat atcgaagaca atcaggctca tctccaaggg gaacccatag aggtggacaa
     3601 tctccctgag gatatggggc gacttcacct ggatgatgga aaatcgccca accatggtga
     3661 gatagccaag gtgggagaag gcaagtatcg agaggacttt cagatggatg aaggagagga
     3721 tcctagcttc ctgttccagt catacctgga aaatgttgga gtccaaatag tcagacaaat
     3781 gaggtcagga gagagatttc tcaagatatg gtcacagacc gtagaagaga ttatatccta
     3841 tgtcgcggtc aactttccca accctccagg aaagtcttca gaggataaat caacccagac
     3901 tactggccga gagctcaaga aggagacaac acccactcct tctcagagag aaagccaatc
     3961 atcgaaagcc aggatggcgg ctcaaattgc ttctggccct ccagcccttg aatggtcggc
     4021 taccaatgaa gaggatgatc tatcagtgga ggctgagatc gctcaccaga ttgcagaaag
     4081 tttctccaaa aaatataagt ttccctctcg atcctcaggg atactcttgt ataattttga
     4141 gcaattgaaa atgaaccttg atgatatagt taaagaggca aaaaatgtac caggtgtgac
     4201 ccgtttagcc catgacgggt ccaaactccc cctaagatgt gtactgggat gggtcgcttt
     4261 ggccaactct aagaaattcc agttgttagt cgaatccgac aagctgagta aaatcatgca
     4321 agatgacttg aatcgctata catcttgcta accgaacctc tcccctcagt ccctctagac
     4381 aataaaatcc gagatgtccc aaagtcaaca tgaaaaaaac aggcaacacc actgataaaa
     4441 tgaacctcct acgtaagata gtgaaaaacc gcagggacga ggacactcaa aaatcctctc
     4501 ccgcgtcagc ccctctggat gacgatgact tgtggcttcc accccctgaa tacgtcccgc
     4561 tgaaagaact tacaggcaag aagaacatga ggaacttttg tatcaacgga agggttaaag
     4621 tgtgtagccc gaatggttac tcgttcagga tcctgcggca cattctgaaa tcattcgacg
     4681 agatatattc tgggaatcat aggatgatcg ggttagtcaa agtggttatt ggactggctt
     4741 tgtcaggatc tccagtccct gagggcctga actgggtata caaattgagg agaaccttta
     4801 tcttccagtg ggctgattcc aggggccctc ttgaagggga ggagttggaa tactctcagg
     4861 agatcacttg ggatgatgat actgagttcg tcggattgca aataagagtg attgcaaaac
     4921 agtgtcatat ccagggcaga gtctggtgta tcaacatgaa cccgagagca tgtcaactat
     4981 ggtctgacat gtctcttcag acacaaaggt ccgaagagga caaagattcc tctctgcttc
     5041 tagaataaac cggatactcc tggaagcctg cccatgctaa gactcttgtg tgatgtatct
     5101 tgaaaaaaac aagatcctaa atctgaacct ttggttgttt gattgttttt ctcatttttg
     5161 ttgtttattt gttaagcgtg ggtcggcatg gcatctccac ctcctcgcgg tccgacctgg
     5221 gcatccgaag gaggacgcac gtccactcgg atggctaagg gagggcgggg atccggctgc
     5281 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
     5341 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
     5401 cggatccact agttctagag cggccgccac cgcggtggag ctccagcttt tgttcccttt
     5461 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     5521 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     5581 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     5641 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     5701 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     5761 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     5821 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     5881 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     5941 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6001 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6061 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     6121 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6181 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     6241 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6301 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     6361 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6421 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6481 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6541 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     6601 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     6661 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     6721 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     6781 ctgcaatgat accgcgagac ccacccttgg aggctccaga tttatcagca ataaaccagc
     6841 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     6901 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     6961 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7021 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7081 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7141 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7201 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     7261 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7321 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7381 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7441 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7501 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7561 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     7621 gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg
     7681 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     7741 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     7801 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     7861 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     7921 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     7981 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     8041 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     8101 atttccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     8161 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     8221 cagggttttc ccagtcacga cgtt
//
