LOCUS       sequence-193894-       10754 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bacterial expression of AsCpf1-2NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10754)
  AUTHORS   Moreno-Mateos MA, Fernandez JP, Rouet R, Vejnar CE, Lane MA, Mis E, 
            Khokha MK, Doudna JA, Giraldez AJ
  TITLE     CRISPR-Cpf1 mediates efficient homology-directed repair and 
            temperature-controlled genome editing.
  JOURNAL   Nat Commun. 2017 Dec 8;8(1):2024. doi: 10.1038/s41467-017-01836-2.
  PUBMED    29222508
REFERENCE   2  (bases 1 to 10754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-12-8"; volume: "8(1):2024. doi"; pages: " 
            10.1038/s41467-017-01836-2"
COMMENT     Sequence Label: AsCpf1-2NLS
FEATURES             Location/Qualifiers
     source          1..10754
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(18..36)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        104..208
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(209..277,278..1069)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(427..446)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1240..1828
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1729..1748
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1982..1999
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2014..2156
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2082..2101)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2242..2264
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2258..2449)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     3907..3926
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4010..4029
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4010..4028
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             4117..4139
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4159..4176
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4186..5286
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     5260..5283
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             5341..5361
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             5371..9291
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     CDS             9295..9321
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             9301..9327
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             9340..9360
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9367..9387
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9427..10131
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(9569..9587)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9837..9856
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             10138..10158
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(10290..10308)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      10304..10351
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(10717..10722,10723..10739,10740..10745))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccaa tgatcgaagt
     3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc
     3721 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     3781 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     3841 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     3901 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     3961 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
     4021 actataggga gaccacaacg gtttccctct agtgccggct ccggagagct ctttaattaa
     4081 gcggccgccc tgcaggactc gagttctaga aataattttg tttaacttta agaaggagat
     4141 atacatatga aatcttctca ccatcaccat caccatggtt cttctatgaa aatcgaagaa
     4201 ggtaaactgg taatctggat taacggcgat aaaggctata acggtctcgc tgaagtcggt
     4261 aagaaattcg agaaagatac cggaattaaa gtcaccgttg agcatccgga taaactggaa
     4321 gagaaattcc cacaggttgc ggcaactggc gatggccctg acattatctt ctgggcacac
     4381 gaccgctttg gtggctacgc tcaatctggc ctgttggctg aaatcacccc ggacaaagcg
     4441 ttccaggaca agctgtatcc gtttacctgg gatgccgtac gttacaacgg caagctgatt
     4501 gcttacccga tcgctgttga agcgttatcg ctgatttata acaaagatct gctgccgaac
     4561 ccgccaaaaa cctgggaaga gatcccggcg ctggataaag aactgaaagc gaaaggtaag
     4621 agcgcgctga tgttcaacct gcaagaaccg tacttcacct ggccgctgat tgctgctgac
     4681 gggggttatg cgttcaagta tgaaaacggc aagtacgaca ttaaagacgt gggcgtggat
     4741 aacgctggcg cgaaagcggg tctgaccttc ctggttgacc tgattaaaaa caaacacatg
     4801 aatgcagaca ccgattactc catcgcagaa gctgccttta ataaaggcga aacagcgatg
     4861 accatcaacg gcccgtgggc atggtccaac atcgacacca gcaaagtgaa ttatggtgta
     4921 acggtactgc cgaccttcaa gggtcaacca tccaaaccgt tcgttggcgt gctgagcgca
     4981 ggtattaacg ccgccagtcc gaacaaagag ctggcaaaag agttcctcga aaactatctg
     5041 ctgactgatg aaggtctgga agcggttaat aaagacaaac cgctgggtgc cgtagcgctg
     5101 aagtcttacg aggaagagtt ggcgaaagat ccacgtattg ccgccactat ggaaaacgcc
     5161 cagaaaggtg aaatcatgcc gaacatcccg cagatgtccg ctttctggta tgccgtgcgt
     5221 actgcggtga tcaacgccgc cagcggtcgt cagactgtcg atgaagccct gaaagacgcg
     5281 cagactaatt cgagctcgaa caacaacaac aataacaata acaacaacct cgggatcgag
     5341 gaaaacctgt acttccaatc caatgccacc atgacccagt tcgaggggtt taccaatctg
     5401 tatcaagtga gcaagacgct gcgctttgaa ctgatcccac agggaaaaac cttaaaacat
     5461 attcaagagc agggctttat cgaagaagat aaggcccgta atgaccatta caaagagtta
     5521 aagccgatta ttgatcgtat ctacaagacc tatgcggacc agtgcttaca attggtacag
     5581 cttgattggg agaacctctc tgccgccatc gattcctatc gtaaagaaaa aactgaagaa
     5641 acgcgcaacg ccctgattga agagcaggcc acctatcgta acgcgattca tgactatttt
     5701 attggccgta cggacaatct gacggacgcg atcaacaagc gccatgcgga gatttacaaa
     5761 ggactgttta aggctgaact gttcaatggt aaggtcctta aacagcttgg gaccgtcaca
     5821 acgacggaac atgaaaacgc gttattacgt agcttcgaca agtttaccac gtatttctcc
     5881 ggcttttacg aaaatcgcaa aaacgttttc agtgccgagg atatttccac tgctatccct
     5941 catcgcattg tgcaagacaa cttcccaaaa ttcaaagaaa attgtcatat cttcacccgc
     6001 ttaatcaccg ctgtaccgtc cctgcgtgag catttcgaaa acgtgaaaaa ggccattggt
     6061 atcttcgtgt ctacttcgat tgaggaggta ttttcctttc cattctataa tcagctgctg
     6121 acccagaccc aaattgatct gtacaaccag ctgcttggcg gtatttctcg tgaagcagga
     6181 accgaaaaaa tcaaagggtt gaacgaggtg cttaatctgg caatccagaa aaatgatgaa
     6241 accgcccaca tcattgcttc gttacctcat cgttttatcc cgttgttcaa gcaaatttta
     6301 agtgatcgca atacgctgtc gtttattctg gaagaattca aaagtgatga agaggtaatt
     6361 cagtcgtttt gcaaatataa aaccctgtta cgtaacgaaa atgtcctgga aacagccgag
     6421 gctttgttta acgaactgaa tagcattgac ctgacgcata tctttattag ccacaaaaaa
     6481 ttagagacca tctcatcagc tctgtgcgat cattgggata cactgcgcaa tgcgctgtat
     6541 gaacgtcgta tttcggaatt gactggcaaa atcactaaaa gcgcgaaaga gaaagtacag
     6601 cgctcgctta aacatgaaga tatcaacctg caggagatca tcagcgccgc gggtaaagaa
     6661 ctgtcggagg catttaaaca gaagacgagc gagattctgt cccacgcaca tgccgcctta
     6721 gaccagccgc tcccgaccac tctgaagaaa caggaagaga aagaaatcct taaaagtcaa
     6781 ctggacagtt tactgggtct ctatcatctg ctggattggt ttgcggtaga cgaaagcaat
     6841 gaagtggatc cggagtttag tgcccgtctg acaggaatca agctggaaat ggagccttcg
     6901 cttagcttct acaacaaagc ccgcaattat gccacgaaaa aaccctatag tgtcgaaaaa
     6961 tttaaactca actttcaaat gccgaccctt gcgtcgggct gggatgtcaa caaagaaaaa
     7021 aacaacggag ctattctgtt cgttaaaaat ggtctgtact acctgggcat catgccgaaa
     7081 cagaaaggtc gctacaaagc cctttcgttc gagcccacgg aaaaaacaag cgaaggcttc
     7141 gacaaaatgt actacgatta ctttccggat gcagcaaaaa tgatcccgaa atgttccaca
     7201 cagctgaaag ccgttacagc acattttcag acgcacacca cccccatctt actgtccaac
     7261 aattttattg aaccgctgga gattactaaa gaaatttatg atttgaacaa tccggaaaaa
     7321 gagccaaaaa agtttcaaac cgcctacgct aaaaaaaccg gggatcagaa agggtaccgc
     7381 gaagcgttgt gcaagtggat tgatttcacc cgcgattttc tcagtaaata taccaagact
     7441 acctcgattg acctgagctc actgcgcccg agctctcaat ataaggattt gggtgagtac
     7501 tatgctgaat taaacccttt attgtaccac atttcttttc agcgcatcgc cgaaaaggaa
     7561 attatggacg cagtcgaaac cgggaaactg tacctgttcc agatctataa taaggacttc
     7621 gccaaaggac atcatggcaa accgaacctg cacacccttt actggaccgg gcttttctct
     7681 ccggaaaatt tggcgaaaac ctcgatcaag cttaacggtc aagctgagct gttttaccgt
     7741 ccaaaatccc gcatgaagcg catggcgcat cgtttaggtg aaaaaatgct gaataagaaa
     7801 ctgaaagatc agaaaacccc tatcccggat accctctacc aggaactgta tgattacgtg
     7861 aaccatcgtc tctcgcatga cctgtcagac gaagcgcgtg cgttactgcc caatgtaatc
     7921 acaaaagaag tttcgcatga aattattaaa gatcgtcgtt ttacatctga taaattcttt
     7981 tttcatgttc cgatcaccct caactatcag gccgcaaaca gtccaagtaa gtttaaccag
     8041 cgcgttaatg cttacctgaa ggaacatccg gagactccga ttattggaat tgatcgcggt
     8101 gaacgtaatt tgatctatat cactgtgatc gatagtaccg gtaagattct ggagcagcgc
     8161 agcttgaaca caattcaaca gtttgattat cagaaaaaat tagacaaccg cgaaaaagag
     8221 cgcgtggctg cccgtcaggc gtggtctgtt gtcggtacca ttaaagatct gaagcagggc
     8281 tatctttctc aggttattca cgaaattgta gatctgatga tccattatca ggcggttgtt
     8341 gtgttggaga atctcaattt cggttttaag agtaagcgca caggcatcgc tgaaaaagca
     8401 gtttatcagc agtttgaaaa aatgctgatc gacaaattga actgtttagt tctcaaagat
     8461 tacccagcgg aaaaggtggg cggagtgctg aatccgtacc aattaacgga tcaattcact
     8521 tccttcgcaa agatgggtac ccaaagcggc tttctgttct atgtgccggc cccgtatacc
     8581 tcgaaaatcg atccactgac gggcttcgta gatccgttcg tgtggaaaac cattaaaaat
     8641 catgaaagtc gtaaacattt tctcgaaggc ttcgacttcc tgcactacga cgtgaaaact
     8701 ggcgatttca ttctgcattt taaaatgaac cgcaaccttt cgtttcagcg cggtctgccg
     8761 ggctttatgc cggcttggga cattgttttt gagaaaaatg aaacccagtt tgatgctaaa
     8821 ggcactcctt tcatcgccgg taaacgcatc gtacctgtga ttgaaaacca tcgttttaca
     8881 gggcgttacc gtgatttata cccggcgaac gaattgatcg cgctgctgga ggaaaagggc
     8941 atcgttttcc gtgacggctc caatattctg ccgaaattac tggaaaacga cgattcacac
     9001 gcaattgata ccatggtcgc actgattcgc tcagtcttac agatgcgtaa ctctaatgca
     9061 gccacaggag aagattatat taattcgcca gtccgcgatt tgaacggtgt ttgcttcgac
     9121 agccgttttc agaatcctga atggccgatg gacgctgatg ccaacggagc ttatcatatc
     9181 gccctgaaag gccagctcct gctgaaccac ctgaaggaaa gcaaagatct gaaattgcag
     9241 aacggcatta gcaaccagga ctggttagca tacatccagg aactgcgtaa cgcctatccc
     9301 tatgacgtgc ccgattatgc cagcctgggc agcggctccc ccaagaaaaa acgcaaggtg
     9361 gaagatccta agaaaaagcg gaaagtggac taaattggta gtgggagcaa cggcagcagc
     9421 ggatccgtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     9481 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     9541 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     9601 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     9661 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     9721 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctccag
     9781 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     9841 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     9901 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     9961 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
    10021 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
    10081 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa ggggtcacct
    10141 aagaaaaaac gaaaagttga gtaataacat tggaagtgga taacggatcc gcgatcgcgg
    10201 cgcgccacct ggtggccggc cggtaccacg cgtgcgcgct gatccggctg ctaacaaagc
    10261 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
    10321 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc
    10381 acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga
    10441 gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa
    10501 attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat
    10561 ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat
    10621 ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct
    10681 gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct
    10741 gtcaaacatg agaa
//
