LOCUS       sequence-193906-        3873 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S. meditearranea NF-YB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Iyer H, Collins JJ 3rd, Newmark PA
  TITLE     NF-YB Regulates Spermatogonial Stem Cell Self-Renewal and 
            Proliferation in the Planarian Schmidtea mediterranea.
  JOURNAL   PLoS Genet. 2016 Jun 15;12(6):e1006109. doi: 
            10.1371/journal.pgen.1006109. eCollection 2016 Jun.
  PUBMED    27304889
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2016-06-15"; volume: "12(6):e1006109. doi"; pages: "
            10.1371/journal.pgen.1006109. eCollection 2016 Jun"
COMMENT     Sequence Label: pHI-Smed-NF-YB
FEATURES             Location/Qualifiers
     source          1..3873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..48)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        84..102
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     84..101
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(752..770)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(774..792)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      806..853
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             1260..2054
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1314..1333)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1924..1943
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(2072..2140,2141..2932)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2290..2309)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3103..3691
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3592..3611
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3845..3862
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctaggc gcgccggatc
       61 ctaatacgac tcactatagg gagatttagg tgacactata gaagtgacct ttggacgata
      121 acatgaatac ttttcttgag cctctttgtt cagcttcata caaagcttgc gatatagatg
      181 gaaatgattg tttgaaagat caggacagat tcctgccgat ttgcaacgtt tcgaaaataa
      241 tgaaaaagga tcttccgttc agtgcaaaga tagccaagga tgctaaacag tgcgttcaag
      301 aatgcgcatc ggaattcatc agctttgtga gcagcgaagc agcagaaatc tgtcaaaatg
      361 acaaaaggaa aacaattaat ggagaagata ttttgcaagc ttttgccaat ttgggatttg
      421 ataattacgt ggagaccttg cagaactttt tgcaaactta tagagaggca aataaatttg
      481 aaaatgatat cattgacctg ggcaacaata ataatgcgca aggcaggcct tggtcaatgg
      541 cgccagtaaa ctcaaactcg tcatcagaac tgaatatcaa actgaacatg cctcatctgc
      601 tttccatgct cccgttgacc gacctcacct cgagcaatct gaatttggaa aatcttcttc
      661 gtgattcaga tttccaaaga gcagttttga atcacttgaa gaaatcatca accgatgaag
      721 actcctttaa tatggatgaa gcaaaggtct ccctttagtg agggttaatt ctccctatag
      781 tgagtcgtat taggatccgg cgcgcctagc ataacccctt ggggcctcta aacgggtctt
      841 gaggggtttt ttgacgcgtc gatttcggcc tattggttaa aaaatgagct gatttaacaa
      901 aaatttaacg cgaattttaa caaaattcag ggcgcaaggg ctgctaaagg aagcggaaca
      961 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
     1021 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
     1081 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
     1141 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa
     1201 tgatctgatg gcgcagggga tcaagatctg atcaagagac aggatgagga tcgtttcgca
     1261 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     1321 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     1381 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     1441 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     1501 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     1561 atctcctgtc atcccacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     1621 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     1681 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     1741 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     1801 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     1861 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     1921 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     1981 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     2041 acgagttctt ctgaattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     2101 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     2161 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     2221 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     2281 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     2341 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     2401 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     2461 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     2521 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     2581 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     2641 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     2701 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     2761 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     2821 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agccaggcaa ctatggatga
     2881 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     2941 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     3001 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     3061 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3121 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3181 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3241 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3301 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3361 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3421 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3481 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3541 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3601 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3661 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3721 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     3781 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     3841 gcgcagcgag tcagtgagcg aggaagcacg cgt
//
