LOCUS       sequence-193908-        3703 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S. meditearranea NF-YB2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Iyer H, Collins JJ 3rd, Newmark PA
  TITLE     NF-YB Regulates Spermatogonial Stem Cell Self-Renewal and 
            Proliferation in the Planarian Schmidtea mediterranea.
  JOURNAL   PLoS Genet. 2016 Jun 15;12(6):e1006109. doi: 
            10.1371/journal.pgen.1006109. eCollection 2016 Jun.
  PUBMED    27304889
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2016-06-15"; volume: "12(6):e1006109. doi"; pages: "
            10.1371/journal.pgen.1006109. eCollection 2016 Jun"
COMMENT     Sequence Label: pHI-Smed-NF-YB2
FEATURES             Location/Qualifiers
     source          1..3703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..48)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        84..102
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     84..101
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(582..600)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(604..622)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      636..683
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             1090..1884
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1144..1163)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1754..1773
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(1902..1970,1971..2762)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2120..2139)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2933..3521
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3422..3441
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3675..3692
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctaggc gcgccggatc
       61 ctaatacgac tcactatagg gagatttagg tgacactata gaagtgacct accaagtcca
      121 gcaaggtgat ggcgaatata ctcaagaatt tgagcaactt tcgccacttc gtgaacaaga
      181 tagatttttg cctattgcaa atgtatccaa aataatgaaa aaagcagtac caacaaatgg
      241 aaaaatatcg aaagaagcaa aagaaatagt acaagaatgt gttagcgaat atattagctt
      301 cattactagc gaagccgctg aaaaatgtca gcaagaaaaa cggaagacga taaatgggga
      361 agacctgtta tgggccatgg caaatttagg atttgaacag agtgtagaac cacttagatt
      421 gtttttaact aagtatcgtg aggctaataa attggattca tcgattatgg acgacagcat
      481 tgaaatggga agacacatgg acaacaatat gggaaatgtt gaaaatacaa tttatctgag
      541 ggataatatt gagtatgttc agagtgaagc cggtaggtct ccctttagtg agggttaatt
      601 ctccctatag tgagtcgtat taggatccgg cgcgcctagc ataacccctt ggggcctcta
      661 aacgggtctt gaggggtttt ttgacgcgtc gatttcggcc tattggttaa aaaatgagct
      721 gatttaacaa aaatttaacg cgaattttaa caaaattcag ggcgcaaggg ctgctaaagg
      781 aagcggaaca cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc
      841 tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt
      901 gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg
      961 ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc
     1021 ttgccgccaa tgatctgatg gcgcagggga tcaagatctg atcaagagac aggatgagga
     1081 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     1141 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     1201 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     1261 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     1321 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     1381 ccggggcagg atctcctgtc atcccacctt gctcctgccg agaaagtatc catcatggct
     1441 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     1501 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     1561 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     1621 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     1681 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     1741 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     1801 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     1861 cgccttcttg acgagttctt ctgaattgaa aaaggaagag tatgagtatt caacatttcc
     1921 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     1981 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     2041 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     2101 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     2161 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     2221 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     2281 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     2341 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     2401 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     2461 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     2521 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     2581 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     2641 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agccaggcaa
     2701 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     2761 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     2821 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     2881 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2941 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3001 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3061 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3121 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3181 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3241 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3301 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3361 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3421 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3481 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3541 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     3601 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     3661 gaacgaccga gcgcagcgag tcagtgagcg aggaagcacg cgt
//
