LOCUS       sequence-19394-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  TITLE     Bokoch Lab GTPases
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGEX-4T3-RhoA-Q63L
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(47..232,233..235,236..628)
                     /codon_start=1
                     /product="constitutively active mutant of human Rho family 
                     small GTPase RhoA"
                     /label=RhoA (Q63L)
                     /translation="MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVAD
                     IEVDGKQVELALWDTAGLEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVK
                     HFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT
                     KDGVREVFEMATRAALQARRGKKKSGCLVL"
ORIGIN
        1 ggttccgcgt ggatccatgg agtatgatgt tcctgattat gctcatatgg ctgccatccg
       61 gaagaaactg gtgattgttg gtgatggagc ctgtggaaag acatgcttgc tcatagtctt
      121 cagcaaggac cagttcccag aggtgtatgt gcccacagtg tttgagaact atgtggcaga
      181 tatcgaggtg gatggaaagc aggtagagtt ggctttgtgg gacacagctg ggctggaaga
      241 ttatgatcgc ctgaggcccc tctcctaccc agataccgat gttatactga tgtgtttttc
      301 catcgacagc cctgatagtt tagaaaacat cccagaaaag tggaccccag aagtcaagca
      361 tttctgtccc aacgtgccca tcatcctggt tgggaataag aaggatcttc ggaatgatga
      421 gcacacaagg cgggagctag ccaagatgaa gcaggagccg gtgaaacctg aagaaggcag
      481 agatatggca aacaggattg gcgcttttgg gtacatggag tgttcagcaa agaccaaaga
      541 tggagtgaga gaggtttttg aaatggctac gagagctgct ctgcaagcta gacgtgggaa
      601 gaaaaaatct ggttgccttg tcttgtgagc ggccgcaagc ttgcatgcct gcaggtcgac
      661 tctagaggat ccccgaattc ccgggtcgac tcgagcggcc gcatcgtgac tgactgacga
      721 tctgcct
//
