LOCUS       sequence-193941-        3767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S. meditearranea NF-YA2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Iyer H, Collins JJ 3rd, Newmark PA
  TITLE     NF-YB Regulates Spermatogonial Stem Cell Self-Renewal and 
            Proliferation in the Planarian Schmidtea mediterranea.
  JOURNAL   PLoS Genet. 2016 Jun 15;12(6):e1006109. doi: 
            10.1371/journal.pgen.1006109. eCollection 2016 Jun.
  PUBMED    27304889
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2016-06-15"; volume: "12(6):e1006109. doi"; pages: "
            10.1371/journal.pgen.1006109. eCollection 2016 Jun"
COMMENT     Sequence Label: pHI-Smed-NF-YA2
FEATURES             Location/Qualifiers
     source          1..3767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..48)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        84..102
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     84..101
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(646..664)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(668..686)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      700..747
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             1154..1948
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1208..1227)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1818..1837
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(1966..2034,2035..2826)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2184..2203)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2997..3585
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3486..3505
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3739..3756
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctaggc gcgccggatc
       61 ctaatacgac tcactatagg gagatttagg tgacactata gaagtgacct ttttaacaac
      121 cagttccgat ggaacatcgt ttcttgcgat acagacagtt gacggtcaca ttatccccat
      181 acaattcgtc acaaatggaa catccaacag ccacaaccta gtaatgatgc cagcaacaac
      241 tattgataat acaaatcaca ctgagagtgg ttctcaagca tctacagatc cagtaaaaga
      301 tgaagcaata aataatgcgg aagaaccgct ttatgtcaac gcaaaacaat ataatcgaat
      361 tttaaaaaga cgagcagcaa gaacaaaatt agaagcccaa ggacgaattc caaaagaaag
      421 gaaaaaatac ctacatgaat caagacataa acacgccatg aatcgaatac gtggatcagg
      481 aggtagattt tattcagttc aaagtgaaat tcaaaatcaa gaaaacaatc aaaaaaatac
      541 aaatgtcact aacaatgaca tatctagaaa gagttatgtc aacggttcac aatcaaatgt
      601 ttcatacgga ttttcacgag aattggtgtc tctagcgaag gtctcccttt agtgagggtt
      661 aattctccct atagtgagtc gtattaggat ccggcgcgcc tagcataacc ccttggggcc
      721 tctaaacggg tcttgagggg ttttttgacg cgtcgatttc ggcctattgg ttaaaaaatg
      781 agctgattta acaaaaattt aacgcgaatt ttaacaaaat tcagggcgca agggctgcta
      841 aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt
      901 cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg
      961 cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga
     1021 attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc
     1081 tttcttgccg ccaatgatct gatggcgcag gggatcaaga tctgatcaag agacaggatg
     1141 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     1201 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     1261 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     1321 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     1381 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     1441 agtgccgggg caggatctcc tgtcatccca ccttgctcct gccgagaaag tatccatcat
     1501 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     1561 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     1621 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     1681 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     1741 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     1801 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     1861 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     1921 ctatcgcctt cttgacgagt tcttctgaat tgaaaaagga agagtatgag tattcaacat
     1981 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     2041 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     2101 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     2161 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     2221 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     2281 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     2341 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     2401 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     2461 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     2521 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     2581 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     2641 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     2701 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagccag
     2761 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     2821 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     2881 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     2941 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3001 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3061 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3121 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     3181 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3241 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3301 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3361 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3421 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3481 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3541 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3601 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3661 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     3721 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cacgcgt
//
