LOCUS       sequence-193942-        3812 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S. meditearranea NF-YC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Iyer H, Collins JJ 3rd, Newmark PA
  TITLE     NF-YB Regulates Spermatogonial Stem Cell Self-Renewal and 
            Proliferation in the Planarian Schmidtea mediterranea.
  JOURNAL   PLoS Genet. 2016 Jun 15;12(6):e1006109. doi: 
            10.1371/journal.pgen.1006109. eCollection 2016 Jun.
  PUBMED    27304889
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2016-06-15"; volume: "12(6):e1006109. doi"; pages: "
            10.1371/journal.pgen.1006109. eCollection 2016 Jun"
COMMENT     Sequence Label: pHI-Smed-NF-YA3
FEATURES             Location/Qualifiers
     source          1..3812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..48)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        84..102
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     84..101
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(691..709)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(713..731)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      745..792
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             1199..1993
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1253..1272)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1863..1882
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(2011..2079,2080..2871)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2229..2248)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3042..3630
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3531..3550
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3784..3801
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctaggc gcgccggatc
       61 ctaatacgac tcactatagg gagatttagg tgacactata gaagtgacct tcgtgaaatc
      121 acattgagag catggattca tactgaacga aataaacggc gaacattaca acgcagtgac
      181 ataacgttgg ctgtcgctga tggtgatacc gatcaatttg atttcttaat tgatattgtt
      241 ccaagagatg aaatcaaaag tcatggctct gccacaaaac gagaagaaac atctcataat
      301 caaccaatga cacaagtcac tgggaattta aacttatttg gaggaaacac tattcagtta
      361 aatactcaag ctttatcgtc agggcaatta ggattagttg attcaaatgg tttatcagtt
      421 ggagctaata taattcaagc tcaaggcaat caatcaaatg ttcaaaatca atctcatgga
      481 gctgtgcagt atgttttaca attaccgatt ggctctaaca attctttatc gggtggtcaa
      541 acaattcaaa ttcaaatgtt acctcagtca atgatatcca attccagcga taatcaaacc
      601 gcgactacaa tgacaacatc cagcaccgtt ttgaatggtc aacaacaatc tcatatattt
      661 gcgattggtg gaaaccagac caaaggtctc cctttagtga gggttaattc tccctatagt
      721 gagtcgtatt aggatccggc gcgcctagca taaccccttg gggcctctaa acgggtcttg
      781 aggggttttt tgacgcgtcg atttcggcct attggttaaa aaatgagctg atttaacaaa
      841 aatttaacgc gaattttaac aaaattcagg gcgcaagggc tgctaaagga agcggaacac
      901 gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct actgggctat
      961 ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg
     1021 gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc
     1081 gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaat
     1141 gatctgatgg cgcaggggat caagatctga tcaagagaca ggatgaggat cgtttcgcat
     1201 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     1261 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     1321 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     1381 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     1441 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     1501 tctcctgtca tcccaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     1561 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     1621 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     1681 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     1741 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     1801 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     1861 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     1921 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     1981 cgagttcttc tgaattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     2041 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     2101 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     2161 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     2221 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     2281 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     2341 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     2401 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     2461 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     2521 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     2581 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     2641 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     2701 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     2761 ggtaagccct cccgtatcgt agttatctac acgacgggga gccaggcaac tatggatgaa
     2821 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     2881 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     2941 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     3001 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3061 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3121 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3181 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3241 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3301 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3361 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3421 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3481 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3541 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3601 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3661 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3721 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3781 cgcagcgagt cagtgagcga ggaagcacgc gt
//
