LOCUS       sequence-193944-        3856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S. mansoni NF-YA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Iyer H, Collins JJ 3rd, Newmark PA
  TITLE     NF-YB Regulates Spermatogonial Stem Cell Self-Renewal and 
            Proliferation in the Planarian Schmidtea mediterranea.
  JOURNAL   PLoS Genet. 2016 Jun 15;12(6):e1006109. doi: 
            10.1371/journal.pgen.1006109. eCollection 2016 Jun.
  PUBMED    27304889
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2016-06-15"; volume: "12(6):e1006109. doi"; pages: "
            10.1371/journal.pgen.1006109. eCollection 2016 Jun"
COMMENT     Sequence Label: pHI-Sm-NF-YA
FEATURES             Location/Qualifiers
     source          1..3856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..48)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        84..102
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(735..753)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(736..753)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(757..775)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      789..836
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(848..865)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1019..1607)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1099..1118)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1778..2569,2570..2638))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2401..2420
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(2656..3450)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2767..2786)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3377..3396
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctaggc gcgccggatc
       61 ctaatacgac tcactatagg gagaattaac cctcactaaa gggagacctt ccggcccaaa
      121 ctattctaca gccgttgcaa cagcctttga taatggatgc aaacggtgcc gctcaaccaa
      181 ctacgttcgc tgttcaggct agcgatggta gcctgattcc cgttcaacta gctacgtctg
      241 ctggtggggg tcaggtcatc atgttccttc caagtgcagc tcagcaaaca atatacacaa
      301 ctcaacctat agtgtctgct gcaaccactt ctttagacgc taaccttaca tcggcccaag
      361 ggcagattat agggacaact gttgaagacg aaactcacat acttcaacaa ttacagcagt
      421 cttcacaagt ccaagttatt caccagaccc agtctggatt atctcaagcc gtttcaaaca
      481 ctcaagttgt tcgggtttct agtccgcaac ccgaaactgc tataggtcag cagggaatgt
      541 ccacacaagc atcctcaaca atatcacctg gtcttactcc tggcggcgaa gaacctcttt
      601 atgttaatgc taaacagtac cacagaattc taaaacgtcg ccaagcacga gcgaaattag
      661 aagcacaagg tcgcattccg aaggaaagac ggaagtatct tcatgagtcc aggcataagc
      721 atgctaaggt cacttctata gtgtcaccta aatctcccta tagtgagtcg tattaggatc
      781 cggcgcgcct agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgacgc
      841 gtgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      901 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
      961 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1021 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1081 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1141 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1201 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1261 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1321 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1381 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1441 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1501 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1561 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1621 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1681 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1741 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     1801 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctggctc cccgtcgtgt
     1861 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     1921 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     1981 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     2041 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     2101 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     2161 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     2221 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     2281 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     2341 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     2401 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     2461 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     2521 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     2581 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     2641 tcttcctttt tcaattcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga
     2701 atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc
     2761 ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg
     2821 gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc
     2881 atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa
     2941 cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc
     3001 ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca
     3061 ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc
     3121 ggcaggagca aggtgggatg acaggagatc ctgccccggc acttcgccca atagcagcca
     3181 gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc
     3241 cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg acaggtcggt
     3301 cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca
     3361 gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga
     3421 acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc
     3481 agatcttgat cccctgcgcc atcagatcat tggcggcaag aaagccatcc agtttacttt
     3541 gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt
     3601 ccataaaacc gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct
     3661 ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca
     3721 gcaccgtttc tgcggactgg ctttctacgt gttccgcttc ctttagcagc ccttgcgccc
     3781 tgaattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     3841 gccgaaatcg acgcgt
//
