LOCUS       sequence-193945-        3694 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Kamitani T, Nguyen HP, Kito K, Fukuda-Kamitani T, Yeh ET
  TITLE     Covalent modification of PML by the sentrin family of ubiquitin-like
            proteins.
  JOURNAL   J Biol Chem. 1998 Feb 6. 273(6):3117-20.
  PUBMED    9452416
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1998 Feb 6. 273(6):3117-20."
COMMENT     Sequence Label: SRa-HA-SUMO1 (Sentrin 1)
FEATURES             Location/Qualifiers
     source          1..3694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(85..105)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(90..419,420..434,435..703)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             435..703
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          743..839
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     promoter        943..961
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     943..960
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             979..1005
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    1369..1490
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1406..1425)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1460..1479
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1524..1541)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1695..2283)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1775..1794)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2454..3245,3246..3314))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3077..3096
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3315..3419)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3487..3505
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3543..3565)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3665..3684
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
       61 tgtaagcgtt aatagcttgg ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agctcctcga tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc
      481 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg
      541 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc
      601 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac
      661 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctc ctcgaggaac
      721 tgaaaaacca gaaagttaac tggtaagttt agtctttttg tcttttattt caggtcccgg
      781 atccggtggt ggtgcaaatc aaagaactgc tcctcagtgg atgttgcctt tacttctagg
      841 cctgtacgga agtgttactt ctgctctaaa agctgcggaa ttcatattgt cgttagaacg
      901 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactatag
      961 aatacaagct agcttatgta cccatacgat gttccggatt acgctagcgg atccatgtct
     1021 gaccaggagg caaaaccttc aactgaggac ttgggggata agaaggaagg tgaatatatt
     1081 aaactcaaag tcattggaca ggatagcagt gagattcact tcaaagtgaa aatgacaaca
     1141 catctcaaga aactcaaaga atcatactgt caaagacagg gtgttccaat gaattcactc
     1201 aggtttctct ttgagggtca gagaattgct gataatcata ctccaaaaga actgggaatg
     1261 gaggaagaag atgtgattga agtttatcag gaacaaacgg ggggtcattc aacagtttag
     1321 tctagagctt gggctgcagc ccgggggatc cagatcttat taaagcagaa cttgtttatt
     1381 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     1441 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     1501 tcgactctag actcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     1561 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     1621 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     1681 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1741 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     1801 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     1861 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1921 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1981 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2041 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     2101 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     2161 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     2221 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2281 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     2341 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     2401 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     2461 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     2521 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     2581 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     2641 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     2701 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     2761 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     2821 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     2881 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     2941 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     3001 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     3061 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     3121 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     3181 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     3241 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     3301 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     3361 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     3421 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     3481 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     3541 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     3601 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     3661 tatgcggcat cagagcagat tgtactgaga gtgc
//
