LOCUS       sequence-193946-        3645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S. mansoni NF-YC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Iyer H, Collins JJ 3rd, Newmark PA
  TITLE     NF-YB Regulates Spermatogonial Stem Cell Self-Renewal and 
            Proliferation in the Planarian Schmidtea mediterranea.
  JOURNAL   PLoS Genet. 2016 Jun 15;12(6):e1006109. doi: 
            10.1371/journal.pgen.1006109. eCollection 2016 Jun.
  PUBMED    27304889
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2016-06-15"; volume: "12(6):e1006109. doi"; pages: "
            10.1371/journal.pgen.1006109. eCollection 2016 Jun"
COMMENT     Sequence Label: pHI-Sm-NF-YC
FEATURES             Location/Qualifiers
     source          1..3645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..48)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        84..102
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(524..542)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(525..542)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(546..564)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      578..625
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(637..654)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(808..1396)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(888..907)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1567..2358,2359..2427))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2190..2209
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(2445..3239)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2556..2575)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3166..3185
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctaggc gcgccggatc
       61 ctaatacgac tcactatagg gagaattaac cctcactaaa gggagacctt tttctcataa
      121 atggattact caagtgatcc actgacagat tgtcagtctc aaatgttatc gttctgggat
      181 cttatacgtg ttgaaataga tggcctgaaa tgtgaccatg ccgctttcaa aacccaggac
      241 ttaccactgg ctcgtattaa aaagattatg aagttggatg atgatatcaa atgtatgatg
      301 ataagcgctg aggcacccat attgttcgca aaagctgctg agcttttcat acgagaacta
      361 acgttgagag catggattca tacagaacgt aaccgccggc gtactcttca aagaaatgat
      421 attgcaatgg cagtcagtga tggagataca gatcaatttg attttcttat tgatattgta
      481 ccacgtgaag aagcaagagg acatcgtcgg ccaacaggtc acttctatag tgtcacctaa
      541 atctccctat agtgagtcgt attaggatcc ggcgcgccta gcataacccc ttggggcctc
      601 taaacgggtc ttgaggggtt ttttgacgcg tgcttcctcg ctcactgact cgctgcgctc
      661 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      721 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      781 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
      841 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      901 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      961 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1021 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1081 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1141 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1201 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1261 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1321 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1381 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1441 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1501 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1561 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     1621 atccatagtt gcctggctcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     1681 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     1741 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     1801 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     1861 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     1921 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     1981 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     2041 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     2101 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     2161 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     2221 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     2281 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     2341 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     2401 ggcgacacgg aaatgttgaa tactcatact cttccttttt caattcagaa gaactcgtca
     2461 agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg
     2521 aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg
     2581 tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca
     2641 ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg
     2701 tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct
     2761 tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg
     2821 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc
     2881 attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgggatga caggagatcc
     2941 tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc
     3001 acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc
     3061 agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct
     3121 gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg
     3181 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg
     3241 cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc ccctgcgcca tcagatcatt
     3301 ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc agagggcgcc
     3361 ccagctggca attccggttc gcttgctgtc cataaaaccg cccagtctag ctatcgccat
     3421 gtaagcccac tgcaagctac ctgctttctc tttgcgcttg cgttttccct tgtccagata
     3481 gcccagtagc tgacattcat ccggggtcag caccgtttct gcggactggc tttctacgtg
     3541 ttccgcttcc tttagcagcc cttgcgccct gaattttgtt aaaattcgcg ttaaattttt
     3601 gttaaatcag ctcatttttt aaccaatagg ccgaaatcga cgcgt
//
