LOCUS       sequence-193947-        3760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S. meditearranea boule1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Iyer H, Issigonis M, Sharma PP, Extavour CG, Newmark PA
  TITLE     A premeiotic function for boule in the planarian Schmidtea 
            mediterranea.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):E3509-18. doi: 
            10.1073/pnas.1521341113. Epub 2016 Jun 2.
  PUBMED    27330085
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1521341113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-21- 21"; volume: "113"; issue: "25"; pages: 
            "E3509-18"
COMMENT     Sequence Label: boule1
FEATURES             Location/Qualifiers
     source          1..3760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..48)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        84..102
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     84..101
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(639..657)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(661..679)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      693..740
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             1147..1941
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1201..1220)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1811..1830
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(1959..2027,2028..2819)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2177..2196)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2990..3578
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3479..3498
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3732..3749
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctaggc gcgccggatc
       61 ctaatacgac tcactatagg gagatttagg tgacactata gaagtgacct ttgcaaacaa
      121 aatgtcaact gatagtcaac ttacgcctaa agtagataca ctagctcaag aatttcaagt
      181 ttcacttgct gctgttaatc aagcaacgat gactgctgct gcaaacccta aaatcggtac
      241 tctgatacca aatcgaatat ttgttggtgg aatcacgtca aatacgacag tagaagaatt
      301 gaaaatattt ttttcaagtt acggtccaat taaggatgtt aaaataataa atgatcgtac
      361 tggattatcc aaagggagtt atgggtttgt tacatttgaa aatcaagaaa ctgctgaaaa
      421 aataatcaaa aatgaagcag aaacactgat atttaaggaa cggaaattaa acataggaca
      481 cgctgttaga aaacagcatt tatttcctcg ccagggttag tatcatgata tgttttaagt
      541 tctgttaagt tgtttaagca acaaaattac attttgtatt tttgctagaa atttctggaa
      601 cagttttata taatggaccc gtgccttatg aaggtctccc tttagtgagg gttaattctc
      661 cctatagtga gtcgtattag gatccggcgc gcctagcata accccttggg gcctctaaac
      721 gggtcttgag gggttttttg acgcgtcgat ttcggcctat tggttaaaaa atgagctgat
      781 ttaacaaaaa tttaacgcga attttaacaa aattcagggc gcaagggctg ctaaaggaag
      841 cggaacacgt agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac
      901 tgggctatct ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg
      961 cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca
     1021 gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg
     1081 ccgccaatga tctgatggcg caggggatca agatctgatc aagagacagg atgaggatcg
     1141 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     1201 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     1261 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     1321 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     1381 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     1441 gggcaggatc tcctgtcatc ccaccttgct cctgccgaga aagtatccat catggctgat
     1501 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     1561 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     1621 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     1681 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     1741 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     1801 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     1861 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     1921 cttcttgacg agttcttctg aattgaaaaa ggaagagtat gagtattcaa catttccgtg
     1981 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     2041 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     2101 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     2161 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     2221 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     2281 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     2341 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     2401 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     2461 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     2521 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     2581 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     2641 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     2701 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagc caggcaacta
     2761 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     2821 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     2881 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     2941 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3001 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3061 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3121 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3181 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3241 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3301 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3361 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3421 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3481 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3541 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3601 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3661 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3721 cgaccgagcg cagcgagtca gtgagcgagg aagcacgcgt
//
