LOCUS       sequence-193956-        3757 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S. meditearranea CDC25-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Iyer H, Issigonis M, Sharma PP, Extavour CG, Newmark PA
  TITLE     A premeiotic function for boule in the planarian Schmidtea 
            mediterranea.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):E3509-18. doi: 
            10.1073/pnas.1521341113. Epub 2016 Jun 2.
  PUBMED    27330085
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1521341113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-21- 21"; volume: "113"; issue: "25"; pages: 
            "E3509-18"
COMMENT     Sequence Label: CDC25-2
FEATURES             Location/Qualifiers
     source          1..3757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..48)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        84..102
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(636..654)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(637..654)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(658..676)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      690..737
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(749..766)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(920..1508)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1000..1019)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1679..2470,2471..2539))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2302..2321
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(2557..3351)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2668..2687)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3278..3297
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctaggc gcgccggatc
       61 ctaatacgac tcactatagg gagaattaac cctcactaaa gggagacctt atgcaatatt
      121 tctgtcagtc aactcaacct gacatggagc aggatctcca gcacgtatta aagttatttg
      181 aatctccaaa ttcttctgaa gatgattttg atacgaaagc tttcatcttt tactgtgaat
      241 tctcatcctt tcgaggacct aaattagctg aatgcctccg aggactggat aagctctcta
      301 atcttgagtc tctcccgaga gtaaaattcc ctcatgttta cgttttgcat ggaggatata
      361 atgagttttt tattaaatat ccagaattat gtactccaca aggatatatt agtatgaaac
      421 agacgcgata tgctttacga tcaccaataa atccgtattt gatggccaat tctccgacca
      481 agcatggtga tctcactgtt gaatccgaca gtgttagagt gcccaacgtc ctcactccga
      541 aactcaccga tgaagttact tgtacattat ataggaagca acagataaac tgccagagac
      601 aattacgatg tgatattaag cgtcttaagg tcacttctat agtgtcacct aaatctccct
      661 atagtgagtc gtattaggat ccggcgcgcc tagcataacc ccttggggcc tctaaacggg
      721 tcttgagggg ttttttgacg cgtgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      781 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      841 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      901 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      961 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1021 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1081 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1141 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1201 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1261 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1321 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1381 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1441 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1501 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1561 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1621 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     1681 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1741 ttgcctggct ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1801 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1861 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1921 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1981 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2041 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2101 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2161 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     2221 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     2281 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     2341 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     2401 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     2461 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     2521 ggaaatgttg aatactcata ctcttccttt ttcaattcag aagaactcgt caagaaggcg
     2581 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     2641 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     2701 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     2761 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
     2821 gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     2881 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     2941 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     3001 agccatgatg gatactttct cggcaggagc aaggtgggat gacaggagat cctgccccgg
     3061 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     3121 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt
     3181 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     3241 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     3301 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga
     3361 tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatca ttggcggcaa
     3421 gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg
     3481 caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc
     3541 actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta
     3601 gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg tgttccgctt
     3661 cctttagcag cccttgcgcc ctgaattttg ttaaaattcg cgttaaattt ttgttaaatc
     3721 agctcatttt ttaaccaata ggccgaaatc gacgcgt
//
