LOCUS       sequence-193957-       12268 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12268)
  AUTHORS   Moore MW, Carbone FR, Bevan MJ
  TITLE     Introduction of soluble protein into the class I pathway of antigen 
            processing and presentation.
  JOURNAL   Cell. 1988 Sep 9. 54(6):777-85.
  PUBMED    3261634
REFERENCE   2  (bases 1 to 12268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1988 
            Sep 9. 54(6):777-85."
COMMENT     Sequence Label: pAc-Neo-OVA
FEATURES             Location/Qualifiers
     source          1..12268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    4384..4440
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             4466..5626
                     /codon_start=1
                     /product="chicken ovalbumin"
                     /label=ovalbumin
                     /translation="MGSIGAASMEFCFDVFKELKVHHANENIFYCPIAIMSALAMVYLG
                     AKDSTRTQINKVVRFDKLPGFGDSIEAQCGTSVNVHSSLRDILNQITKPNDVYSFSLAS
                     RLYAEERYPILPEYLQCVKELYRGGLEPINFQTAADQARELINSWVESQTNGIIRNVLQ
                     PSSVDSQTAMVLVNAIVFKGLWEKAFKDEDTQAMPFRVTEQESKPVQMMYQIGLFRVAS
                     MASEKMKILELPFASGTMSMLVLLPDEVSGLEQLESIINFEKLTEWTSSNVMEERKIKV
                     YLPRMKMEEKYNLTSVLMAMGITDVFSSSANLSGISSAESLKISQAVHAAHAEINEAGR
                     EVVGSAEAGVDAASVSEEFRADHPFLFCIKHIATNAVLFFGRCVSP"
     polyA_signal    6323..6457
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6600..6622)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6833..6850)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7004..7592)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7084..7103)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7763..8554,8555..8623))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8386..8405
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8624..8728)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8796..8814
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8834..8854)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8839..9168
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9019..9154
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9529..10323
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9583..9602)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10193..10212
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     intron          10743..10808
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10938..10958
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11383..11517
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 aattccgact ttcaaacagc ctttggactc aggccttggg gacattccag gggacccttt
       61 accagggagg ggcagtgtga ggcaggaggc agggctgccg cctcagggac cctgagccta
      121 gagtccttcc caccgcattt ggggacttga gcacttcacc agtcctgact cagtttccta
      181 gtctgtgaat gggggtgtgt ctcctggaat gttgcccccc ctcattcatt cattctcctc
      241 tcactgagca cctaccgtgt tcagggtccc tgtcctccta gagctaactc aagtcttgga
      301 gggaagctct gtctcagttc cttcctctac aaaatggagc ttgaggacgg gcacggtggc
      361 tcatgcctgt aatcccagga ctttgggagg ctgaggtggg aggatcgctt gagccccccg
      421 gagctggaga ccagcctggg caacatggtg agaagccata cctctacaga taatttaaaa
      481 attagccagg cttggaagtg tgtgcctata gtcccagcta ctcgtgaagg ctttgagccc
      541 aggaggtcga ggctgcagtg agctgtgatc gcactccagc ctgggcatca cagcaagacc
      601 ttgtatcaaa aatagtaata ataataaaaa ggaggttgga ttccctcctg gcaggatagg
      661 gagggcgctg cagtgcccag ggcaaggtgg ctgggtggtt gttttgcggg agggccaagg
      721 agtggtccct gggtctgcgc tgtaagagtt ggttgcctgg aggccttgca gggtgggggt
      781 gtcaccaaag caaaggcttg gaggggaaaa aacaaaagtc ccagccaggc aggtggagtc
      841 cccttggctg agttcaaccg agggttctcc gggggctgcg tgcgtgcccc agtgacagct
      901 ccgaaagctc ccttacaggg caaagttccc aagcacagaa gagaacctgt tcacttctcc
      961 cctgctcggc ccgccccctg gccaggcacc tctacttcct cttttcctgc tccgctgctt
     1021 gctttctctc ttcagctcct ccctgcccct caccccaggc tgctcggcca cctccaacct
     1081 gccacctgag gacacccagg cagtcactca ttcaacagcg aggagccctg gggtgggtgt
     1141 agtgggaagg agtgggggtg acggagaccc tgggagggct cgcagcctgg tggctgaggc
     1201 ccagttctaa atgccagctg caagccttgg tctgaggtag ggaggaaggc gtggctgcag
     1261 aggctaaaac gcttccccaa agaggggctt tctgggatgg gacttgaagg gtgcatagga
     1321 gagcactagg aagtggccgc tgcagacaga gggaaccaca agccaggagg acaggccagg
     1381 aatgctgcag cccggggcgg ggtggggctg gagctcctgt ctcttggcca gctgaatgga
     1441 ggcccagcgg caacacaggt cctgcctggg gatcaggtct gctctgcacc ccaccttgct
     1501 gcctggagcc gcccacctga caacctctca tccctgctct gtagatccgg tcccatcccc
     1561 actgcccacc ccaccccccc agcactccac ccagttcaac gttccacgaa cccccagaac
     1621 cagccctcat caacaggcag caagaagggc cccccgccca tcgccccaca acgccagccg
     1681 ggtgaactgt agcgttggca ggtcctgagg cagctggcaa gacgcctgca gctgaaagat
     1741 acaaggccag ggacaggaca gtcccatccc caggaggcag ggagtataca ggctggggaa
     1801 gtttgccctt gcgtggggtg gtgatggagg aggctcagca agtcttctgg actgtgaacc
     1861 tgtgtctgcc actgtgtgct gggtggtggt catctttccc accaggctgt ggcctctgca
     1921 accttcaagg gaggagcagg tcccattggc tgagcacagc cttgtaccgt gaactggaac
     1981 aagcagcctc cttcctggcc acaggttcca tgtccttata tggactcatc tttgcctatt
     2041 gcgacacaca ctcaatgaac acctactacg cgctgcaaag agccccgcag gcctgaggtg
     2101 cccccacctc accactcttc ctatttttgt gtaaaaatcc agcttcttgt caccacctcc
     2161 aaggaggggg aggaggagga aggcaggttc ctctaggctg agccgaatgc ccctctgtgg
     2221 tcccacgcca ctgatcgctg catgcccacc acctgggtac acacagtctg tgattcccgg
     2281 agcagaacgg accctgccca cccggtcttg tgtgctactc agtggacaga cccaaggcaa
     2341 gaaagggtga caaggacagg gtcttcccag gctggctttg agttcctagc accgccccgc
     2401 ccccaatcct ctgtggcaca tggagtcttg gtccccagag tcccccagcg gcctccagat
     2461 ggtctgggag ggcagttcag ctgtggctgc gcatagcaga catacaacgg acggtgggcc
     2521 cagacccagg ctgtgtagac ccagcccccc cgccccgcag tgcctaggtc acccactaac
     2581 gccccaggcc tggtcttggc tgggcgtgac tgttaccctc aaaagcaggc agctccaggg
     2641 taaaaggtgc cctgccctgt agagcccacc ttccttccca gggctgcggc tgggtaggtt
     2701 tgtagccttc atcacgggcc acctccagcc actggaccgc tggcccctgc cctgtcctgg
     2761 ggagtgtggt cctgcgactt ctaagtggcc gcaagccacc tgactccccc aacaccacac
     2821 tctacctctc aagcccaggt ctctccctag tgacccaccc agcacattta gctagctgag
     2881 ccccacagcc agaggtcctc aggccctgct ttcagggcag ttgctctgaa gtcggcaagg
     2941 gggagtgact gcctggccac tccatgccct ccaagagctc cttctgcagg agcgtacaga
     3001 acccagggcc ctggcacccg tgcagaccct ggcccacccc acctgggcgc tcagtgccca
     3061 agagatgtcc acacctagga tgtcccgcgg tgggtggggg gcccgagaga cgggcaggcc
     3121 gggggcaggc ctggccatgc ggggccgaac cgggcactgc ccagcgtggg gcgcgggggc
     3181 cacggcgcgc gcccccagcc cccgggccca gcaccccaag gcggccaacg ccaaaactct
     3241 ccctcctcct cttcctcaat ctcgctctcg ctcttttttt ttttcgcaaa aggaggggag
     3301 agggggtaaa aaaatgctgc actgtgcggc gaagccggtg agtgagcggc gcggggccaa
     3361 tcagcgtgcg ccgttccgaa agttgccttt tatggctcga gcggccgcgg cggcgcccta
     3421 taaaacccag cggcgcgacg cgccaccacc gccgagaccg cgtccgcccc gcgagcacag
     3481 agcctcgcct ttgccgatcc gccgcccgtc cacacccgcc gccaggtaag cccggccagc
     3541 cgaccggggc atgcggccgc ggccccttcg cccgtgcaga gccgccgtct gggccgcagc
     3601 ggggggcgca tgggggggga accggaccgc cgtggggggc gcgggagaag cccctgggcc
     3661 tccggagatg ggggacaccc cacgccagtt cggaggcgcg aggccgcgct cgggaggcgc
     3721 gctccggggg tgccgctctc ggggcggggg caaccggcgg ggtctttgtc tgagccgggc
     3781 tcttgccaat ggggatcgca gggtgggcgc ggcgtagccc ccgccaggcc cggtgggggc
     3841 tggggcgcca ttgcgcgtgc gcgctggtcc tttgggcgct aactgcgtgc gcgctgggaa
     3901 ttggcgctaa ttgcgcgtgc gcgctgggac tcaaggcgct aattgcgcgt gcgttctggg
     3961 gcccggggtg ccgcggcctg ggctggggcg aaggcgggct cggccggaag gggtggggtc
     4021 gccgcggctc ccgggcgctt gcgcgcactt cctgcccgag ccgctggccg cccgagggtg
     4081 tggccgctgc gtgcgcgcgc gccgacccgg cgctgtttga accgggcgga ggcggggctg
     4141 gcgcccggtt gggagggggt tggggcctgg cttcctgccg cgcgccgcgg ggacgcctcc
     4201 gaccagtgtt tgccttttat ggtaataacg cggccggccc ggcttccttt gtccccaatc
     4261 tgggcgcgcg ccggcgcccc ctggcggcct aaggactcgg cgcgccggaa gtggccaggg
     4321 cgggggcgac ttcggctcac agcgcgcccg gctattctcg caggatcagt cgacctgcag
     4381 cccaagcttg catgcctgca ggtcgactct agaggatccc cgggtaccga gctcgaattc
     4441 ctcgaaagac aactcagagt tcaccatggg ctccatcggt gcagcaagca tggaattttg
     4501 ttttgatgta ttcaaggagc tcaaagtcca ccatgccaat gagaacatct tctactgccc
     4561 cattgccatc atgtcagctc tagccatggt atacctgggt gcaaaagaca gcaccaggac
     4621 acaaataaat aaggttgttc gctttgataa acttccagga ttcggagaca gtattgaagc
     4681 tcagtgtggc acatctgtaa acgttcactc ttcacttaga gacatcctca accaaatcac
     4741 caaaccaaat gatgtttatt cgttcagcct tgccagtaga ctttatgctg aagagagata
     4801 cccaatcctg ccagaatact tgcagtgtgt gaaggaactg tatagaggag gcttggaacc
     4861 tatcaacttt caaacagctg cagatcaagc cagagagctc atcaattcct gggtagaaag
     4921 tcagacaaat ggaattatca gaaatgtcct tcagccaagc tccgtggatt ctcaaactgc
     4981 aatggttctg gttaatgcca ttgtcttcaa aggactgtgg gagaaagcat ttaaggatga
     5041 agacacacaa gcaatgcctt tcagagtgac tgagcaagaa agcaaacctg tgcagatgat
     5101 gtaccagatt ggtttattta gagtggcatc aatggcttct gagaaaatga agatcctgga
     5161 gcttccattt gccagtggga caatgagcat gttggtgctg ttgcctgatg aagtctcagg
     5221 ccttgagcag cttgagagta taatcaactt tgaaaaactg actgaatgga ccagttctaa
     5281 tgttatggaa gagaggaaga tcaaagtgta cttacctcgc atgaagatgg aggaaaaata
     5341 caacctcaca tctgtcttaa tggctatggg cattactgac gtgtttagct cttcagccaa
     5401 tctgtctggc atctcctcag cagagagcct gaagatatct caagctgtcc atgcagcaca
     5461 tgcagaaatc aatgaagcag gcagagaggt ggtagggtca gcagaggctg gagtggatgc
     5521 tgcaagcgtc tctgaagaat ttagggctga ccatccattc ctcttctgta tcaagcacat
     5581 cgcaaccaac gccgttctct tctttggcag atgtgtttcc ccttaaaaag aagaaagctg
     5641 aaaaactctg tcccttccaa caagacccag agcactgtag tatcaggggt aaaatgaaaa
     5701 gtatgttatc tgctgcatcc agacttcata aaagctggag cttaatctag aaaaaaaatc
     5761 agaaagaaat tacactgtga gaacaggtgc aattcacttt tcctttacac agagtaatac
     5821 tggtaactca tggatgaagg cttaagggaa tgaaattgga ctcacagtac tgagtcatca
     5881 cactgaaaaa tgcaacctga tacatcagca gaaggtttat gggggaaaaa tgcagccttc
     5941 caattaagcc agatatctgt atgaccaagc tgctccagaa ttagtcactc aaaatctctc
     6001 agattaaatt atcaactgtc accaaccatt cctatgctga caaggcaatt gcttgttctc
     6061 tgtgttcctg atactacaag gctcttcctg acttcctaaa gatgcattat aaaaatctta
     6121 taattcacat ttctccctaa actttgactc aatcatggta tgttggcaaa tatggtatat
     6181 tactattcaa attgttttcc ttgtacccat atgtaatggg tcttgtgaat gtgctctttt
     6241 gttcctttaa tcataataaa aacatgttta agcaaaaaaa aaaaaaaaaa aaaaaaaaag
     6301 gaattcgccc atgggcgccc cagatccaga catgataaga tacattgatg agtttggaca
     6361 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     6421 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     6481 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     6541 atgtggtatg gctgattatg atccggctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     6601 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     6661 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     6721 cagtcacgta gcgggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     6781 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
     6841 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6901 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6961 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7021 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7081 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7141 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7201 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7261 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7321 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7381 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7441 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7501 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7561 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7621 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7681 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7741 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7801 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7861 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7921 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7981 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8041 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct
     8101 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8161 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8221 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8281 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8341 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
     8401 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8461 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8521 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8581 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8641 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8701 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     8761 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     8821 ttatcatcgc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     8881 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     8941 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     9001 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     9061 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     9121 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctagcttca
     9181 cgctgccgca agcactcagg gcgcaagggc tgctaaagga agcggaacac gtagaaagcc
     9241 agtccgcaga aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg
     9301 gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta
     9361 gactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt
     9421 aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg
     9481 cgcaggggat caagatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     9541 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     9601 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     9661 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     9721 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     9781 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     9841 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     9901 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     9961 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
    10021 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
    10081 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
    10141 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
    10201 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
    10261 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
    10321 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
    10381 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
    10441 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccgggc
    10501 tcgatcccct cgcgagttgg ttcagctgct gcctgaggct ggacgacctc gcggagttct
    10561 accggcagtg caaatccgtc ggcatccagg aaaccagcag cggctatccg cgcatccatg
    10621 cccccgaact gcaggagtgg ggaggcacga tggccgcttt ggtccggatc tttgtgaagg
    10681 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
    10741 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
    10801 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
    10861 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
    10921 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
    10981 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
    11041 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
    11101 ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt tttcttactc
    11161 cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct
    11221 ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc
    11281 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
    11341 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
    11401 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    11461 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcgat
    11521 ccccaggaag ctcctctgtg tcctcataaa ccctaacctc ctctacttga gaggacattc
    11581 caatcatagg ctgcccatcc accctctgtg tcctcctgtt aattaggtca cttaacaaaa
    11641 aggaaattgg gtaggggttt ttcacagacc gctttctaag ggtaatttta aaatatctgg
    11701 gaagtccctt ccactgctgt gttccagaag tgttggtaaa cagcccacaa atgtcaacag
    11761 cagaaacata caagctgtca gctttgcaca agggcccaac accctgctca tcaagaagca
    11821 ctgtggttgc tgtgttagta atgtgcaaaa caggaggcac attttcccca cctgtgtagg
    11881 ttccaaaata tctagtgttt tcatttttac ttggatcagg aacccagcac tccactggat
    11941 aagcattatc cttatccaaa acagccttgt ggtcagtgtt catctgctga ctgtcaactg
    12001 tagcattttt tggggttaca gtttgagcag gatatttggt cctgtagttt gctaacacac
    12061 cctgcagctc caaaggttcc ccaccaacag caaaaaaatg aaaatttgac ccttgaatgg
    12121 gttttccagc accattttca tgagtttttt gtgtccctga atgcaagttt aacatagcag
    12181 ttaccccaat aacctcagtt ttaacagtaa cagcttccca catcaaaata tttccacagg
    12241 ttaagtcctc atttaaatta ggcaaagg
//
