LOCUS       sequence-193960-        6835 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6835)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 6835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP3.1.2
FEATURES             Location/Qualifiers
     source          1..6835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1840..1857)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1846..2070
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2202..2790)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2282..2301)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3119..3166
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3191..3210
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3398..4192)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(3398..4192)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3509..3528)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4119..4138
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4227..4584)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4241..4376
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4393..4584
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4541..4561
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(4644..5435,5436..5504))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5267..5286
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5505..5609)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5636..6091
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5723..5742)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5933..5954
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        6103..6482
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6483..6686
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6637..6657
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6731..6750
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6731..6749
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggatccatgg gttgctcttt ttctatcttc cttcttgctc tgttctcttg cctaatccat
       61 ccagcagcta gtttagagtg gcggaacacg tctggcctct acatccttac caacgactgc
      121 cccaatagca gtattgtgta tgaggccgat gacgttattc tgcacacacc tggctgtata
      181 ccttgtgttc aggacggcaa tacatccacg tgctggacct cagtgacacc tacagtggca
      241 gtcagatacg tcggagctac caccgcttcg atacgcagtc atgtagacct gctggtaggc
      301 gcggccacga tgtgctctgc gctctacgtg ggtgacgcgt gtggggccgt cttcctcgtg
      361 ggacaggcct tcacgttcag accccgtcgc catcaaacgg tccagacctg caactgctcg
      421 ctgtacccag gccatctcac gggacatcga atggcttggg atatgatgat gaattggtcc
      481 cccgctgtgg gtatggtagt ggcgcacgtc ctgcgtctgc cccagacctt gttcgacata
      541 atagccgggg cccattgggg catcttggcg ggcctagcct attactccat gcagggcaac
      601 tgggccaagg tcgttatcat catggttatg ttttcagggg ttgacgcttc tacatacacc
      661 accggtggcc aagcagctcg tggtgcccgt gggatagctg gtctttttga tctgggcgcc
      721 cgacagaacc tgcagctggt taacaccaat ggctcgtggc acatcaacag gacagccctg
      781 aactgcaatg agtccataaa cacggggttc atagcagggt tgttttacta ccataagttc
      841 aactctactg ggtgtcctca aaggctcagc agctgcaagc ccatcacttc cttcaggcag
      901 gggtggggcc ctttgacaga tgctaacatc accggttcct ctgatgacaa accgtactgc
      961 tggcactacg cacctagacc ttgtgagtct gtcccagcat caaaagtctg cggccctgtg
     1021 tactgcttca caccatcgcc agtggttgta ggcactactg atgctaaggg cgtcccaacc
     1081 tacacctggg gtgcgaatga gacagacgtg ttcctgctga attccctgcg gcctcccaaa
     1141 ggtcggtggt tcgggtgcac ctggatgaac tctacggggt ttaccaagac gtgcggagcc
     1201 cccccttgta acatctatgg gggtgggggg aattccaaaa atgagtcaga cctcttctgc
     1261 cccaccgact gcttcaggaa acatcctgag gccacataca gccggtgtgg tgcggggccc
     1321 tggttgacac ctcggtgcat ggtcgactat ccataccggc tttggcacta cccatgtaca
     1381 gtcaatttca cattgttcca ggtgaggatg tttgtgggcg ggtttgaaca ccggtttacc
     1441 gccgcttgca actggaccag gggggagcgc tgcgatatcg aggatcgcga ccgcagcgag
     1501 caacatccgc tgctacattc aacaactgag ctcgctatac tgccttgctc cttcacgccc
     1561 atgcccgcat tgtcgacagg tctaatacac ctccaccaaa acatcgtgga tgtccaatac
     1621 ctctatggcg ttggatctgg catggtggga tgggcgttga aatgggagtt cgttgtcctc
     1681 gtcttcctcc tcctagcaga tgcacgcgtg tgcgttgccc tttggctgat gctgatgatt
     1741 tcacaggcgg aagcataagc ttataagccg aattctgcag atatccagca cagtggcggc
     1801 cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     1861 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     1921 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     1981 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     2041 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agtggcggta
     2101 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     2161 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     2221 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2281 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2341 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2401 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2461 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2521 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2581 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2641 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     2701 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     2761 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     2821 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     2881 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     2941 gagtaacctg aggctatggc agggcctgcc gccccgacgt tggctgcgag ccctgggcct
     3001 tcacccgaac ttggggggtg gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa
     3061 tggggtctcg gtggggtatc gacagagtgc cagccctggg accgaacccc gcgtttatga
     3121 acaaacgacc caacaccgtg cgttttattc tgtcttttta ttgccgtcat agcgcgggtt
     3181 ccttccggta ttgtctcctt ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac
     3241 tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa aacgattccg
     3301 aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc
     3361 gtcgcttggt cggtcatttc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc
     3421 gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt
     3481 cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat
     3541 agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca
     3601 ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca
     3661 tgctcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca
     3721 gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt
     3781 tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat
     3841 cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg
     3901 gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg
     3961 cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct tgcagttcat
     4021 tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc
     4081 ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc
     4141 tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg
     4201 atcctcatcc tgtctcttga tcgatctttg caaaagccta ggcctccaaa aaagcctcct
     4261 cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa
     4321 attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc
     4381 ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg
     4441 cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt
     4501 tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc
     4561 cacagctggt tctttccgcc tcaggactct tcctttttca ataaatcaat ctaaagtata
     4621 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     4681 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     4741 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     4801 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     4861 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4921 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     4981 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     5041 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     5101 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     5161 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     5221 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     5281 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     5341 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     5401 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     5461 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     5521 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5581 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg
     5641 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5701 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5761 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5821 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5881 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5941 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     6001 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6061 aattttaaca aaatattaac gcttacaatt tacgcgcgcg ttgacattga ttattgacta
     6121 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     6181 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     6241 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     6301 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     6361 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     6421 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     6481 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     6541 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     6601 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     6661 ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact gcttactggc
     6721 ttatcgaaat taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa
     6781 gcttggtacc gagctcggat ccactagtcc agtgtggtgg aattcggctt tcata
//
