LOCUS       sequence-193972-        2855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of GFP spaghetti monster myc construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Viswanathan S, Williams ME, Bloss EB, Stasevich TJ, Speer CM, Nern 
            A, Pfeiffer BD, Hooks BM, Li WP, English BP, Tian T, Henry GL, 
            Macklin JJ, Patel R, Gerfen CR, Zhuang X, Wang Y, Rubin GM, Looger 
            LL
  TITLE     High-performance probes for light and electron microscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):568-76. doi: 10.1038/nmeth.3365. Epub 
            2015 Apr 27.
  PUBMED    25915120
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3365"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "568-76"
COMMENT     Sequence Label: pAAV.CAG.GFPsm-myc.WPRE.SV40
FEATURES             Location/Qualifiers
     source          1..2855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(8..596)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(88..107)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(767..1558,1559..1627))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1390..1409
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1628..1732)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1758..2213)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1895..1916)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2107..2126
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   2285..2414
                     /label=AAV2 ITR
ORIGIN
        1 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
       61 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      121 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      181 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      241 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      301 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      361 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
      421 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
      481 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
      541 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
      601 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
      661 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
      721 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
      781 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
      841 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
      901 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
      961 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     1021 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     1081 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     1141 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     1201 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     1261 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     1321 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     1381 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     1441 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     1501 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     1561 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     1621 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     1681 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     1741 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     1801 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     1861 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     1921 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     1981 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     2041 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     2101 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     2161 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
     2221 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     2281 ccagctgcgc gctcgctcgc tcactgaggc cgcccgggca aagcccgggc gtcgggcgac
     2341 ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat
     2401 cactaggggt tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg
     2461 ctctaggaag agtaccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     2521 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     2581 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2641 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2701 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
     2761 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
     2821 cgatgggggc gggggggggg ggggggcgcg cgcca
//
