LOCUS       sequence-19399-e         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  TITLE     Bokoch Lab GTPases
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGEX-2T-Cdc42-T17N
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(18..65,66..68,69..593)
                     /codon_start=1
                     /product="dominant negative mutant of human Rho family 
                     small GTPase Cdc42"
                     /label=Cdc42 (T17N)
                     /translation="MQTIKCVVVGDGAVGKNCLLISYTTNKFPSEYVPTVFDNYAVTVM
                     IGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHH
                     CPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQK
                     GLKNVFDEAILAALEPPEPKKSRRCVLL"
     primer_bind     complement(682..704)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tggttccgcg tggatccatg cagacaatta agtgtgttgt tgtgggcgat ggtgctgttg
       61 gtaaaaattg tctcctgata tcctacacaa caaacaaatt tccatcggaa tatgtaccga
      121 ctgtttttga caactatgca gtcacagtta tgattggtgg agaaccatat actcttggac
      181 tttttgatac tgcagggcaa gaggattatg acagattacg accgctgagt tatccacaaa
      241 cagatgtatt tctagtctgt ttttcagtgg tctctccatc ttcatttgaa aacgtgaaag
      301 aaaagtgggt gcctgagata actcaccact gtccaaagac tcctttcttg cttgttggga
      361 ctcaaattga tctcagagat gacccctcta ctattgagaa acttgccaag aacaaacaga
      421 agcctatcac tccagagact gctgaaaagc tggcccgtga cctgaaggct gtcaagtatg
      481 tggagtgttc tgcacttaca cagaaaggcc taaagaatgt atttgacgaa gcaatattgg
      541 ctgccctgga gcctccagaa ccgaagaaga gccgcaggtg tgtgctgcta tgaggatccc
      601 cgaattcccg ggtcgactcg agcggccgca tcgtgactga ctgacgatct gcctcgcgcg
      661 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
      721 tct
//
