LOCUS       sequence-19400-e         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  TITLE     Bokoch Lab GTPases
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGEX-2T-Cdc42-Q61L
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             5..22
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             join(23..202,203..205,206..598)
                     /codon_start=1
                     /product="constitutively active mutant of human Rho family 
                     small GTPase Cdc42"
                     /label=Cdc42 (Q61L)
                     /translation="MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVM
                     IGGEPYTLGLFDTAGLEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHH
                     CPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQK
                     GLKNVFDEAILAALEPPEPKKSRRCVLL"
     primer_bind     complement(687..709)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ggatctggtt ccgcgtggat ccatgcagac aattaagtgt gttgttgtgg gcgatggtgc
       61 tgttggtaaa acatgtctcc tgatatccta cacaacaaac aaatttccat cggaatatgt
      121 accgactgtt tttgacaact atgcagtcac agttatgatt ggtggagaac catatactct
      181 tggacttttt gatactgcag ggctagagga ttatgacaga ttacgaccgc tgagttatcc
      241 acaaacagat gtatttctag tctgtttttc agtggtctct ccatcttcat ttgaaaacgt
      301 gaaagaaaag tgggtgcctg agataactca ccactgtcca aagactcctt tcttgcttgt
      361 tgggactcaa attgatctca gagatgaccc ctctactatt gagaaacttg ccaagaacaa
      421 acagaagcct atcactccag agactgctga aaagctggcc cgtgacctga aggctgtcaa
      481 gtatgtggag tgttctgcac ttacacagaa aggcctaaag aatgtatttg acgaagcaat
      541 attggctgcc ctggagcctc cagaaccgaa gaagagccgc aggtgtgtgc tgctatgagg
      601 atccccgaat tcccgggtcg actcgagcgg ccgcatcgtg actgactgac gatctgcctc
      661 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
      721 gcttgtctgt aagcggatgc cgggagc
//
