LOCUS       sequence-194013-        6581 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes sequence of the murine H2-Kb allele.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6581)
  AUTHORS   Kelton W, Waindok AC, Pesch T, Pogson M, Ford K, Parola C, Reddy ST
  TITLE     Reprogramming MHC specificity by CRISPR-Cas9-assisted cassette 
            exchange.
  JOURNAL   Sci Rep. 2017 Apr 4;7:45775. doi: 10.1038/srep45775.
  PUBMED    28374766
REFERENCE   2  (bases 1 to 6581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-04-4"; volume: "7:45775. doi"; pages: " 
            10.1038/srep45775"
COMMENT     Sequence Label: pUC19, containing H2-Kb c57/BL6 WK9
FEATURES             Location/Qualifiers
     source          1..6581
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     4417..4439
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4477..4495)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4563..4667
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4668..4736,4737..5528)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4886..4905)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5699..6287
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6188..6207
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6441..6458
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gaaccaatca gtgtcgccgc ggacgctgga tataaagtcc acgcagcccg cagaactcag
       61 aagtcgcgaa tcgccgacag gtgcgatggt accgtgcacg ctgctcctgc tgttggcggc
      121 cgccctggct ccgactcaga cccgcgcggg tgagtaccgg gccgggaggg aaacggcctc
      181 tgaggggagg ggcggcaccg gggaagccgc gtcctcgggt cgcccaccgg accctccgcc
      241 ccttctccac ccgagtcccg cgccctgctc ccctctcagc ccgcgcagcc gcccggggtc
      301 tggtgaggtg gtccgggtct caccgcgcgc cgcccccagg cccacactcg ctgaggtatt
      361 tcgtcaccgc cgtgtcccgg cccggcctcg gggagccccg gtacatggaa gtcggctacg
      421 tggacgacac ggagttcgtg cgcttcgaca gcgacgcgga gaatccgaga tatgagccgc
      481 gggcgcggtg gatggagcag gaggggcccg agtattggga gcgggagaca cagaaagcca
      541 agggcaatga gcagagtttc cgagtggacc tgaggaccct gctcggctac tacaaccaga
      601 gcaagggcgg tgagtgaccc cgggtcggag gtcacgaccc ctccacgtcc cgacacaggg
      661 acgctgacgt cccgggtccc aagtccgagg ttcgggaaca gaacggaccc ggaaccggtt
      721 tctctttcag tttggaggag tccgcgggcg ggcggggccg gggcgggtga gcggggctga
      781 ccgcggggtc ccgcaggctc tcacactatt caggtgatct ctggctgtga agtggggtcc
      841 gacgggcgac tcctccgcgg gtaccagcag tacgcctacg acggctgcga ttacatcgcc
      901 ctgaacgaag acctgaaaac gtggacggcg gcgggcatgg cggcgctgat caccaaacac
      961 aagtgggagc aggctggtga agcagagaga ctcagggcct acctggaggg cacgtgcgtg
     1021 gagtggctcc gcagatacct gaaggacggg aacgcgacgc tgctgcgcac aggtgcaggg
     1081 gccgcgggca gctcctccct ctgccctcgg gctggggctc agtcctgggg aagaagaaac
     1141 cctcagctgg ggtgatgccc ctgtctcaga ggggagagag tgtcgctggt ctcctgatcc
     1201 ctcatcacag tgactgcact gactctccca gggctcagcc ttctccctgg acagtgccca
     1261 ggctgtctca ggagggaagg agagaatttc cctgaggtaa caacagctgc tcccttcagt
     1321 tcccctgcag cctctgtcag ccatggcctc tcccaggccg ggttctctgc ccacgcccac
     1381 tgtctgtaga cactgactcc tgtcctgctg aatgtgtcag cccttacacc tcaggaccgg
     1441 aagtctcctt acctgataag agacatggac acctctacac taggacggtt cacctagttt
     1501 ctagaatttt ccaaagaata cattctcaca gatccctccc tgtctgtggg gttttgcacc
     1561 ccttaacaac ccaattctat ctattcctac agtggtgaat ggtcacatga gcccttatgg
     1621 gctaccctgg aggaatataa atcgtggatt ttcttctctc tctctctctc cctctctttc
     1681 cgtttttttt tttttttttt tttttttttt ttcctagacg gggtttctct gtatagccct
     1741 ggctgtcctg gaactcactt tgtagaccag gttggccttg aactcagaaa tctgcctgcc
     1801 tctggctccc gagtgctggg attaaaggca tgcgccacca ctcctggcct ttcttttttc
     1861 ttaacttttt ttttggtggg gggggggctt attttgtttc tagtcagctt ttgtctgcat
     1921 tggagtgatc cagtctctcc atgccctcct attatcattt gtttcagtct ccacaggtgc
     1981 cagggaaggc tgctaacctg gataattaga caagttaaat cagggttcga attaaagaag
     2041 agattcctgt gagcttagac tgtttcctgt cagaactaaa catgcagaag cctcctgctc
     2101 ttcctctgcc ccacaagtta cagtgctccc tcccgctagt aaatcaggac ttggactctg
     2161 agaggcaggg tcttctgcaa tccagggctg agtgagaggg aagaccacac accctgtgag
     2221 cccactgtgt tccagtgagt gctgcactgg ggtccacagc acactccagg gatcctgtgt
     2281 gacacacctg taccttgtcc cccagagtca ggggctggga gtcattttct ctggctacac
     2341 acttagtgat ggctgttcac ttggactgac agttaatgtt ggtcagcaag gtgactacaa
     2401 tggttgagtc tcaatggtgt caccttccag gatcatacag ccctaatttt aatatgaact
     2461 caaacacata ttaaattagt tattttccat tccctcctcc attctttgac tacctctctc
     2521 atgctattga acatcacata aggatggcca tgtttaccca atggctcatg tggattccct
     2581 cttagcttct gagtcccaaa agaaaatgtg cagtcctgtg ctgaggggac cagctctgct
     2641 tttggtcact agtgcgatga cagttgaagt gtcaaacaga cacatagttc actgtcatca
     2701 ttgatttaac tgagtcttgg gtagatttca gtttgtcttg ttaattgtgt gatttcttaa
     2761 atcttccaca cagattcccc aaaggcccat gtgacccatc acagcagacc tgaagataaa
     2821 gtcaccctga ggtgctgggc cctgggcttc taccctgctg acatcaccct gacctggcag
     2881 ttgaatgggg aggagctgat ccaggacatg aagcttgtgg agaccaggcc tgcaggggat
     2941 ggaaccttcc agaagtgggc atctgtggtg gtgcctcttg ggaaggagca gtattacaca
     3001 tgccatgtgt accatcaggg gctgcctgag cccctcaccc tgagatgggg taaggagagt
     3061 gtgggtgcag agctggggtc agggaaagct ggagctttct gcagaccctg agctgctcag
     3121 ggctgagagc tggggtcatg accctcacct tcatttcttg tacctgtcct tcccagagcc
     3181 tcctccatcc actgtctcca acatggcgac cgttgctgtt ctggttgtcc ttggagctgc
     3241 aatagtcact ggagctgtgg tggcttttgt gatgaagatg agaaggagaa acacaggtag
     3301 gaaagggcag agtctgagtt ttctctcagc ctcctttaga gtgtgctctg ctcatcaatg
     3361 gggaacacag gcacacccca cattgctact gtctctaact gggtctgctg tcagttctgg
     3421 gaacttccta gtgtcaagat cttcctggaa ctctcacagc ttttcttctc acaggtggaa
     3481 aaggagggga ctatgctctg gctccaggtt agtgtgggga cagagttgtc ctggggacat
     3541 tggagtgaag ttggagatga tgggagctct gggaatccat aatagctcct ccagagaaat
     3601 cttctaggtg cctgagttgt gccatgaaat gaatatgtac atgtacatat gcatatacat
     3661 ttgttttgtt ataccctagg ctcccagacc tctgatctgt ctctcccaga ttgtaaaggt
     3721 gacactctag ggtctgattg gggaggggca atgtggacat gattgggttt caggaactcc
     3781 cagaatcccc tgtgagcgag tgatgggttg ttcgaatgtt gtcttcacag tgatggttca
     3841 tgaccctcat tctctagcgt gaagacagct gcctggagtg gacttggtga cagacaatgt
     3901 cttctcatat ctcctgtgac atccagagcc ctcagttctc tttagtcaag tgtctgatgt
     3961 tccctgtgag cctatggact caatgtgaag aactgtggag cccagtccac ccctctacac
     4021 caggaccctg tccctgcact gctctgtctt cccttccaca gccaaccttg ctggttcagc
     4081 caaacactga gggacatctg tagcctgtca gctccatgct accctgacct gcaactcctc
     4141 acttccacac tgagaataat aatttgaatg taaccttgat tgttatcatc acgacctagg
     4201 gctgatttct tgttaatttc atggattgag aatgcttaga ggttttgttt gtttgtttgg
     4261 ttgatttgtt tttttgaaga aataaatgat agatgaataa acttccagaa tctgggtcac
     4321 tattaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     4381 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     4441 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     4501 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     4561 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4621 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4681 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4741 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     4801 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     4861 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     4921 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     4981 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5041 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5101 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5161 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5221 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5281 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5341 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5401 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5461 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5521 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     5581 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     5641 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5701 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5761 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5821 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     5881 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     5941 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6001 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6061 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6121 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6181 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6241 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6301 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6361 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     6421 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     6481 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     6541 ccgactggaa agcgggcagt gagcgcaacg caattaatgt g
//
