LOCUS       sequence-19402-e         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  TITLE     Bokoch Lab GTPases
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRK5-myc-Cdc42-T17N
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             48..77
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(84..131,132..134,135..659)
                     /codon_start=1
                     /product="dominant negative mutant of human Rho family 
                     small GTPase Cdc42"
                     /label=Cdc42 (T17N)
                     /translation="MQTIKCVVVGDGAVGKNCLLISYTTNKFPSEYVPTVFDNYAVTVM
                     IGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHH
                     CPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQK
                     GLKNVFDEAILAALEPPEPKKSRRCVLL"
ORIGIN
        1 ccaggtccaa ctgcacctcg gttctatcga ttgaattggc caccatggag cagaagctga
       61 tctccgagga ggacctggga tccatgcaga caattaagtg tgttgttgtg ggcgatggtg
      121 ctgttggtaa aaattgtctc ctgatatcct acacaacaaa caaatttcca tcggaatatg
      181 taccgactgt ttttgacaac tatgcagtca cagttatgat tggtggagaa ccatatactc
      241 ttggactttt tgatactgca gggcaagagg attatgacag attacgaccg ctgagttatc
      301 cacaaacaga tgtatttcta gtctgttttt cagtggtctc tccatcttca tttgaaaacg
      361 tgaaagaaaa gtgggtgcct gagataactc accactgtcc aaagactcct ttcttgcttg
      421 ttgggactca aattgatctc agagatgacc cctctactat tgagaaactt gccaagaaca
      481 aacagaagcc tatcactcca gagactgctg aaaagctggc ccgtgacctg aaggctgtca
      541 agtatgtgga gtgttctgca cttacacaga aaggcctaaa gaatgtattt gacgaagcaa
      601 tattggctgc cctggagcct ccagaaccga agaagagccg caggtgtgtg ctgctatgag
      661 gatccgcggc cgcgctagcg aattcct
//
