LOCUS       sequence-19403-e         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  TITLE     Bokoch Lab GTPases
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRK5-myc-Cdc42-Q61L
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             22..51
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(58..237,238..240,241..633)
                     /codon_start=1
                     /product="constitutively active mutant of human Rho family 
                     small GTPase Cdc42"
                     /label=Cdc42 (Q61L)
                     /translation="MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVM
                     IGGEPYTLGLFDTAGLEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHH
                     CPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQK
                     GLKNVFDEAILAALEPPEPKKSRRCVLL"
     regulatory      653..662
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    666..747
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(703..722)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tcgattgaat tggccaccat ggagcagaag ctgatctccg aggaggacct gggatccatg
       61 cagacaatta agtgtgttgt tgtgggcgat ggtgctgttg gtaaaacatg tctcctgata
      121 tcctacacaa caaacaaatt tccatcggaa tatgtaccga ctgtttttga caactatgca
      181 gtcacagtta tgattggtgg agaaccatat actcttggac tttttgatac tgcagggcta
      241 gaggattatg acagattacg accgctgagt tatccacaaa cagatgtatt tctagtctgt
      301 ttttcagtgg tctctccatc ttcatttgaa aacgtgaaag aaaagtgggt gcctgagata
      361 actcaccact gtccaaagac tcctttcttg cttgttggga ctcaaattga tctcagagat
      421 gacccctcta ctattgagaa acttgccaag aacaaacaga agcctatcac tccagagact
      481 gctgaaaagc tggcccgtga cctgaaggct gtcaagtatg tggagtgttc tgcacttaca
      541 cagaaaggcc taaagaatgt atttgacgaa gcaatattgg ctgccctgga gcctccagaa
      601 ccgaagaaga gccgcaggtg tgtgctgcta tgagaattcc tgcagaagct tggccgccat
      661 ggcccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
      721 tcacaaataa agcatttttt tcactgcat
//
