LOCUS       sequence-194033-        5684 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ppsR2-bphP1 bicistronic operon plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Ong NTX, Olson EJ, Tabor JJ
  TITLE     Engineering an E. coli near-infrared light sensor.
  JOURNAL   ACS Synth Biol. 2017 Nov 1. doi: 10.1021/acssynbio.7b00289.
  PUBMED    29091422
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Nov 1. doi: 10.1021/acssynbio.7b00289."
COMMENT     Sequence Label: pNO183
FEATURES             Location/Qualifiers
     source          1..5684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(181..275)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(296..955)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     870..889
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1124..1142
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      4873..4944
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4960..4987
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(join(5189..5684,1..93))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5269..5288)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
       61 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
      121 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgac tagtgcttgg
      181 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
      241 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
      301 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
      361 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
      421 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
      481 ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa
      541 taaacccttt agggaaatag gccaggtttt caccataaca cgccacatct tgcgaatata
      601 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
      661 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
      721 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
      781 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
      841 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
      901 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
      961 cttccttagc tcctgaaaat ctcgataact caaaaaatac gaccggtagt gatcttattt
     1021 cattatggtg aaagttggaa cctcttacgg gccggccaac gtctcatttt cgccagatat
     1081 cgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     1141 gccctttcgt cttcacctcg agaaaaagag tattgactta aagtctaacc tataggtata
     1201 atgtgtggac atcgcgtccc aatagctcta gagaaggtta ccatggcgtc aaagtccgtt
     1261 catgccgaca tcacccttct gctcgatatg gagggtgtga ttcgcgaagc caccctgtct
     1321 ccgacgatgg cggccgagag cgtggacggt tggctggggc gtcgctggag cgacatcgcc
     1381 ggcgccgaag gcggcgacaa ggttcgccgc atggtcgaag acgcccgccg cagcggcatc
     1441 tcggctttcc gccagatcaa tcagcctttc ccgagcggcg tcgaaatccc gatcgaattc
     1501 accacgatgc tgctgggcga tcgcaccggc atgatcgcgg tcggcaagaa catgcaggcg
     1561 gtcaccgagc tgcattcccg gctgatcgct gcgcagcagg cgatggagcg cgactattgg
     1621 cggttgcgtg aattggagac tcgctaccgc ctggtgttcg acgctgccgc cgatgcggtg
     1681 atgatcgtct ccgccggcga catgcgcatc gtcgaagcca accgggcggc ggtgaatgcg
     1741 atcagccgcg tcgagcgcgg caatgacgac cttgcggggc gtgatttcct cgccgaagtg
     1801 gcggctgccg atcgcgatgc ggtgcgcgac atgctggccc aggtgcgtca gcgcggcacc
     1861 gcactcagcg tcctcgttca tctcggccgt tacgaccgcg cctggatgct gcgcggttcg
     1921 ctgatgtcgt ccgagcgtcg tcaggttttc ctgctgcact tcaccccggt gaccacgact
     1981 cccgcgatcg acgacgtcga cgatgatgcc gtgctgcgcg ggctgatcga tcgcattccc
     2041 gacgggttcg tcgcactgga ttcggaaggc gtcgttcgtc acgccaacca ggcgtttctc
     2101 gatctggtcc agatcggctc caagcctgcg gcggtcggac gatcgctggg cgtctggatg
     2161 ggtcgtccgg gcgccgatct gtccagcttg ctgacgctgc tgcggcgcta caagacggtg
     2221 cggctgttcc aaacgacgat ccgcggcgag ctcggcaccg agactgaagt cgaggtgtcg
     2281 gccgtcgacg gcgaggacga ccaatacatc ggcgttctga tgcgcaatgt cgcgcgacgc
     2341 ctcgacgctg cggacgacca cgatgccttg cgtcaggcgc tcggcccgat cagcaagcag
     2401 ctcgggcgat cctcgctgcg caagctggtg aagaacgccg tgagcattgt cgagcagcac
     2461 tacgtgaagg aagcgctgtt gcgatccaag ggcaatcgca cggcaactgc cgaactgctc
     2521 ggattgagcc ggcagagcct ttatgcaaaa ctcaactgct acggcttcga cgacaaaggt
     2581 gtcgttgctt ctgctgccga cggtgcagag ggcgcctcag acgacgcaga ggattgagac
     2641 accatctaca aaatagatag aggataatat ggcaggtcat gcctctggca gccccgcatt
     2701 cgggaccgcc gatctttcga attgcgaacg tgaagagatc cacctcgccg gctcgatcca
     2761 gccgcatggc gcgcttctgg tcgtcagcga gccggatcat cgcatcatcc aggccagcgc
     2821 caacgccgcg gaatttctga atctcggaag cgtgctcggc gttccgctcg ccgagatcga
     2881 cggcgatctg ttgatcaaga tcctgccgca tctcgatccc accgccgaag gcatgccggt
     2941 cgcggtgcgc tgccggatcg gcaatccctc cacggagtac gacggtctga tgcatcggcc
     3001 tccggaaggc gggctgatca tcgagctcga acgtgccggc ccgccgatcg atctgtccgg
     3061 cacgctggcg ccggcgctgg agcggatccg cacggcgggc tcgctgcgcg cgctgtgcga
     3121 tgacaccgcg ctgctgtttc agcagtgcac cggctacgac cgggtgatgg tgtatcgctt
     3181 cgacgagcag ggccacggcg aagtgttctc cgagcgccac gtgcccgggc tcgaatccta
     3241 tttcggcaac cgctatccgt cgtcggacat tccgcagatg gcgcggcggc tgtacgagcg
     3301 gcagcgcgtc cgcgtgctgg tcgacgtcag ctatcagccg gtgccgctgg agccgcggct
     3361 gtcgccgctg accgggcgcg atctcgacat gtcgggctgc ttcctgcgct cgatgtcgcc
     3421 gatccatctg cagtacctga agaacatggg cgtgcgcgcc accctggtgg tgtcgctggt
     3481 ggtcggcggc aagctgtggg gcctggttgc ctgtcaccat tatctgccgc gcttcatcca
     3541 tttcgagctg cgggcgatct gcgaactgct cgccgaagcg atcgcgacgc ggatcaccgc
     3601 gcttgagagc ttcgcgcaga gccagtcgga gctgttcgtg cagcggctcg aacagcgcat
     3661 gatcgaagcg atcacccgtg aaggcgattg gcgcgcagcg attttcgaca ccagccaatc
     3721 gatcctgcag ccgctgcacg ccgacggttg cgcgctggtg tacgaagacc agatcaggac
     3781 catcggtgac gtaccttcca cgcaggatgt tcgcgagatc gccgggtggc tcgatcgcca
     3841 gccacgtgcg gcggtgacct cgaccgcgtc gctcggactc gacgtgccgg agctcgcgca
     3901 tctgacgcgg atggcgagcg gcgtggtcgc ggcgccgatt tcggatcatc gcggcgagtt
     3961 tctgatgtgg ttccgccccg agcgcgtcca caccgttacc tggggcggcg atccgaagaa
     4021 gccgttcacg atgggcgata caccggcgga tctgtcgccg cggcgctcct tcgccaaatg
     4081 gcatcaggtt gtcgaaggca cgtccgatcc gtggacggcc gccgatctcg ccgcggctcg
     4141 caccatcggt cagaccgtcg ccgacatcgt gctgcaattc cgcgcggtgc ggacactgat
     4201 cgcccgcgaa cagtacgaac agttttcgtc ccaggtgcac gcttcgatgc agccggtgct
     4261 gatcaccgac gccgaaggcc gcatcctgct gatgaacgac tcgttccgcg acatgttgcc
     4321 ggcggggtcg ccatccgccg tccatctcga cgatctcgcc gggttcttcg tcgaatcgaa
     4381 cgatttcctg cgcaacgtcg ccgaactgat cgatcacggc cgcgggtggc gcggcgaagt
     4441 tctgctgcgc ggcgcaggta atcgcccgtt gccgctggca gtgcgcgccg atccggtgac
     4501 gcgcacggag gaccagtcgc tcggcttcgt gctgatcttc agcgacgcta ccgatcgtcg
     4561 caccgcagat gccgcacgca cgcgtttcca ggaaggcatt cttgccagcg cacgtcccgg
     4621 cgtgcggctc gactccaagt ccgacctctt gcacgagaag ctgctgtccg cgctggtcga
     4681 gaacgcgcag cttgccgcat tggaaattac ttacggcgtc gaaaccggac gcatcgccga
     4741 gctgctcgaa ggcgttcgcc agtcgatgct gcgcaccgcc gaagtgctcg gccatctggt
     4801 gcagcacgcg gcgcgcacgg ccggcagcga cagctcgagc aatggctcgc agaacaagaa
     4861 gtagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggactt tcgttttatc
     4921 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     4981 ttctgcgttt ataggtaccc gtggatcctc tagagtcgac ctgcaggcat gcaagcttcc
     5041 taggcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     5101 cagaatcagg ggataacgca tgcaagaaca tgtgagcaaa aggccagcaa aaggccagga
     5161 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     5221 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     5281 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     5341 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     5401 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     5461 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     5521 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     5581 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     5641 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agtt
//
