LOCUS       sequence-19404-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  TITLE     Bokoch Lab GTPases
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGEX-2T-Rac1-wt
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             31..609
                     /codon_start=1
                     /product="human Rac family small GTPase 1"
                     /label=Rac1
                     /translation="MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     primer_bind     complement(668..690)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ctcaaatcgg atctggttcc gcgtggatcc atgcaggcca tcaagtgtgt ggtggtggga
       61 gacggagctg taggtaaaac ttgcctactg atcagttaca caaccaatgc atttcctgga
      121 gaatatatcc ctactgtctt tgacaattat tctgccaatg ttatggtaga tggaaaaccg
      181 gtgaatctgg gcttatggga tacagctgga caagaagatt atgacagatt acgcccccta
      241 tcctatccgc aaacagatgt gttcttaatt tgcttttccc ttgtgagtcc tgcatcattt
      301 gaaaatgtcc gtgcaaagtg gtatcctgag gtgcggcacc actgtcccaa cactcccatc
      361 atcctagtgg gaactaaact tgatcttagg gatgataaag acacgatcga gaaactgaag
      421 gagaagaagc tgactcccat cacctatccg cagggtctag ccatggctaa ggagattggt
      481 gctgtaaaat acctggagtg ctcggcgctc acacagcgag gcctcaagac agtgtttgac
      541 gaagcgatcc gagcagtcct ctgcccgcct cccgtgaaga agaggaagag aaaatgcctg
      601 ctgttgtaag aattcatcgt gactgactga cgatctgcct cgcgcgtttc ggtgatgacg
      661 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
      721 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
      781 ccatgaccca gtcacgt
//
