LOCUS       sequence-194050-       15465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All ATGs from the start of gag to NC mutated to ATC except the first
            which was mutated to ACG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15465)
  AUTHORS   McCauley SM, Kim K, Nowosielska A, Dauphin A, Yurkovetskiy L, Diehl 
            WE, Luban J
  TITLE     Intron-containing RNA from the HIV-1 provirus activates type I 
            interferon and inflammatory cytokines.
  JOURNAL   Nat Commun. 2018 Dec 13;9(1):5305. doi: 10.1038/s41467-018-07753-2.
  PUBMED    30546110
REFERENCE   2  (bases 1 to 15465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-12-13"; volume: "9(1):5305. doi"; pages: " 
            10.1038/s41467-018-07753-2"
COMMENT     Sequence Label: pNL4-3 envFS eGFP NoStarts
FEATURES             Location/Qualifiers
     source          1..15465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             78..258
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     CDS             417..1916
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="GARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFAV
                     NPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKIE
                     EEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQIVHQAISPRTLNAWVKVVEEKAF
                     SPEVIPIFSALSEGATPQDLNTILNTVGGHQAAIQILKETINEEAAEWDRLHPVHAGPI
                     APGQIREPRGSDIAGTTSTLQEQIGWITHNPPIPVGEIYKRWIILGLNKIVRIYSPTSI
                     LDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWITETLLVQNANPDCKTILKALGPGA
                     TLEEIITACQGVGGPGHKARVLAEAISQVTNPATIIIQKGNFRNQRKTVKCFNCGKEGH
                     IAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAPP
                     EESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             1709..4720
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    4405..4522
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7385..7618
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7803..7847
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8141..8167
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             join(8434..8436,8437..9150)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(8434..8436,8437..9147)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8476..8497)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8737..8756)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9084..9105
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             9340..9973
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11613..11630)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11784..12372)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11864..11883)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12543..13334,13335..13403))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13166..13185
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13404..13508)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13576..13594
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(13632..13654)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     13754..13773
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gaggtgtggc ctgggcggga ctggggagtg gcgagccctc agatgctaca tataagcagc
       61 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
      121 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg ctcaaagtag
      181 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
      241 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag taaagccaga
      301 ggagatctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
      361 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagacgggtg
      421 cgagagcgtc ggtattaagc gggggagaat tagataaatg ggaaaaaatt cggttaaggc
      481 cagggggaaa gaaacaatat aaactaaaac atatagtatg ggcaagcagg gagctagaac
      541 gattcgcagt taatcctggc cttttagaga catcagaagg ctgtagacaa atactgggac
      601 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacaatag
      661 cagtcctcta ttgtgtgcat caaaggatag atgtaaaaga caccaaggaa gccttagata
      721 agatagagga agagcaaaac aaaagtaaga aaaaggcaca gcaagcagca gctgacacag
      781 gaaacaacag ccaggtcagc caaaattacc ctatagtgca gaacctccag gggcaaatcg
      841 tacatcaggc catatcacct agaactttaa atgcatgggt aaaagtagta gaagagaagg
      901 ctttcagccc agaagtaata cccatctttt cagcattatc agaaggagcc accccacaag
      961 atttaaatac catcctaaac acagtggggg gacatcaagc agccatccaa atcttaaaag
     1021 agaccatcaa tgaggaagct gcagaatggg atagattgca tccagtgcat gcagggccta
     1081 ttgcaccagg ccagatcaga gaaccaaggg gaagtgacat agcaggaact actagtaccc
     1141 ttcaggaaca aataggatgg atcacacata atccacctat cccagtagga gaaatctata
     1201 aaagatggat aatcctggga ttaaataaaa tagtaagaat ctatagccct accagcattc
     1261 tggacataag acaaggacca aaggaaccct ttagagacta tgtagaccga ttctataaaa
     1321 ctctaagagc cgagcaagct tcacaagagg taaaaaattg gatcacagaa accttgttgg
     1381 tccaaaatgc gaacccagat tgtaagacta ttttaaaagc attgggacca ggagcgacac
     1441 tagaagaaat catcacagca tgtcagggag tggggggacc cggccataaa gcaagagttt
     1501 tggctgaagc aatcagccaa gtaacaaatc cagctaccat aatcatacag aaaggcaatt
     1561 ttaggaacca aagaaagact gttaagtgtt tcaattgtgg caaagaaggg cacatagcca
     1621 aaaattgcag ggcccctagg aaaaagggct gttggaaatg tggaaaggaa ggacaccaaa
     1681 tgaaagattg tactgagaga caggctaatt ttttagggaa gatctggcct tcccacaagg
     1741 gaaggccagg gaattttctt cagagcagac cagagccaac agccccacca gaagagagct
     1801 tcaggtttgg ggaagagaca acaactccct ctcagaagca ggagccgata gacaaggaac
     1861 tgtatccttt agcttccctc agatcactct ttggcagcga cccctcgtca caataaagat
     1921 aggggggcaa ttaaaggaag ctctattaga tacaggagca gatgatacag tattagaaga
     1981 aatgaatttg ccaggaagat ggaaaccaaa aatgataggg ggaattggag gttttatcaa
     2041 agtaagacag tatgatcaga tactcataga aatctgcgga cataaagcta taggtacagt
     2101 attagtagga cctacacctg tcaacataat tggaagaaat ctgttgactc agattggctg
     2161 cactttaaat tttcccatta gtcctattga gactgtacca gtaaaattaa agccaggaat
     2221 ggatggccca aaagttaaac aatggccatt gacagaagaa aaaataaaag cattagtaga
     2281 aatttgtaca gaaatggaaa aggaaggaaa aatttcaaaa attgggcctg aaaatccata
     2341 caatactcca gtatttgcca taaagaaaaa agacagtact aaatggagaa aattagtaga
     2401 tttcagagaa cttaataaga gaactcaaga tttctgggaa gttcaattag gaataccaca
     2461 tcctgcaggg ttaaaacaga aaaaatcagt aacagtactg gatgtgggcg atgcatattt
     2521 ttcagttccc ttagataaag acttcaggaa gtatactgca tttaccatac ctagtataaa
     2581 caatgagaca ccagggatta gatatcagta caatgtgctt ccacagggat ggaaaggatc
     2641 accagcaata ttccagtgta gcatgacaaa aatcttagag ccttttagaa aacaaaatcc
     2701 agacatagtc atctatcaat acatggatga tttgtatgta ggatctgact tagaaatagg
     2761 gcagcataga acaaaaatag aggaactgag acaacatctg ttgaggtggg gatttaccac
     2821 accagacaaa aaacatcaga aagaacctcc attcctttgg atgggttatg aactccatcc
     2881 tgataaatgg acagtacagc ctatagtgct gccagaaaag gacagctgga ctgtcaatga
     2941 catacagaaa ttagtgggaa aattgaattg ggcaagtcag atttatgcag ggattaaagt
     3001 aaggcaatta tgtaaacttc ttaggggaac caaagcacta acagaagtag taccactaac
     3061 agaagaagca gagctagaac tggcagaaaa cagggagatt ctaaaagaac cggtacatgg
     3121 agtgtattat gacccatcaa aagacttaat agcagaaata cagaagcagg ggcaaggcca
     3181 atggacatat caaatttatc aagagccatt taaaaatctg aaaacaggaa agtatgcaag
     3241 aatgaagggt gcccacacta atgatgtgaa acaattaaca gaggcagtac aaaaaatagc
     3301 cacagaaagc atagtaatat ggggaaagac tcctaaattt aaattaccca tacaaaagga
     3361 aacatgggaa gcatggtgga cagagtattg gcaagccacc tggattcctg agtgggagtt
     3421 tgtcaatacc cctcccttag tgaagttatg gtaccagtta gagaaagaac ccataatagg
     3481 agcagaaact ttctatgtag atggggcagc caatagggaa actaaattag gaaaagcagg
     3541 atatgtaact gacagaggaa gacaaaaagt tgtcccccta acggacacaa caaatcagaa
     3601 gactgagtta caagcaattc atctagcttt gcaggattcg ggattagaag taaacatagt
     3661 gacagactca caatatgcat tgggaatcat tcaagcacaa ccagataaga gtgaatcaga
     3721 gttagtcagt caaataatag agcagttaat aaaaaaggaa aaagtctacc tggcatgggt
     3781 accagcacac aaaggaattg gaggaaatga acaagtagat aaattggtca gtgctggaat
     3841 caggaaagta ctatttttag atggaataga taaggcccaa gaagaacatg agaaatatca
     3901 cagtaattgg agagcaatgg ctagtgattt taacctacca cctgtagtag caaaagaaat
     3961 agtagccagc tgtgataaat gtcagctaaa aggggaagcc atgcatggac aagtagactg
     4021 tagcccagga atatggcagc tagattgtac acatttagaa ggaaaagtta tcttggtagc
     4081 agttcatgta gccagtggat atatagaagc agaagtaatt ccagcagaga cagggcaaga
     4141 aacagcatac ttcctcttaa aattagcagg aagatggcca gtaaaaacag tacatacaga
     4201 caatggcagc aatttcacca gtactacagt taaggccgcc tgttggtggg cggggatcaa
     4261 gcaggaattt ggcattccct acaatcccca aagtcaagga gtaatagaat ctatgaataa
     4321 agaattaaag aaaattatag gacaggtaag agatcaggct gaacatctta agacagcagt
     4381 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     4441 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     4501 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggaa
     4561 aggaccagca aagctcctct ggaaaggtga aggggcagta gtaatacaag ataatagtga
     4621 cataaaagta gtgccaagaa gaaaagcaaa gatcatcagg gattatggaa aacagatggc
     4681 aggtgatgat tgtgtggcaa gtagacagga tgaggattaa cacatggaaa agattagtaa
     4741 aacaccatat gtatatttca aggaaagcta aggactggtt ttatagacat cactatgaaa
     4801 gtactaatcc aaaaataagt tcagaagtac acatcccact aggggatgct aaattagtaa
     4861 taacaacata ttggggtctg catacaggag aaagagactg gcatttgggt cagggagtct
     4921 ccatagaatg gaggaaaaag agatatagca cacaagtaga ccctgaccta gcagaccaac
     4981 taattcatct gcactatttt gattgttttt cagaatctgc tataagaaat accatattag
     5041 gacgtatagt tagtcctagg tgtgaatatc aagcaggaca taacaaggta ggatctctac
     5101 agtacttggc actagcagca ttaataaaac caaaacagat aaagccacct ttgcctagtg
     5161 ttaggaaact gacagaggac agatggaaca agccccagaa gaccaagggc cacagaggga
     5221 gccatacaat gaatggacac tagagctttt agaggaactt aagagtgaag ctgttagaca
     5281 ttttcctagg atatggctcc ataacttagg acaacatatc tatgaaactt acggggatac
     5341 ttgggcagga gtggaagcca taataagaat tctgcaacaa ctgctgttta tccatttcag
     5401 aattgggtgt cgacatagca gaataggcgt tactcgacag aggagagcaa gaaatggagc
     5461 cagtagatcc tagactagag ccctggaagc atccaggaag tcagcctaaa actgcttgta
     5521 ccaattgcta ttgtaaaaag tgttgctttc attgccaagt ttgtttcatg acaaaagcct
     5581 taggcatctc ctatggcagg aagaagcgga gacagcgacg aagagctcat cagaacagtc
     5641 agactcatca agcttctcta tcaaagcagt aagtagtaca tgtaatgcaa cctataatag
     5701 tagcaatagt agcattagta gtagcaataa taatagcaat agttgtgtgg tccatagtaa
     5761 tcatagaata taggaaaata ttaagacaaa gaaaaataga caggttaatt gatagactaa
     5821 tagaaagagc agaagacagt ggcaatgaga gtgaaggaga agtatcagca cttgtggaga
     5881 tgggggtgga aatggggcac catgctcctt gggatattga tgatctgtag tgctacagaa
     5941 aaattgtggg tcacagtcta ttatggggta cctgtgtgga aggaagcaac caccactcta
     6001 ttttgtgcat cagatgctaa agcatatatg atacagaggt acataatgtt tgggccacac
     6061 atgcctgtgt acccacagac cccaacccac aagaagtagt attggtaaat gtgacagaaa
     6121 attttaacat gtggaaaaat gacatggtag aacagatgca tgaggatata atcagtttat
     6181 gggatcaaag cctaaagcca tgtgtaaaat taaccccact ctgtgttagt ttaaagtgca
     6241 ctgatttgaa gaatgatact aataccaata gtagtagcgg gagaatgata atggagaaag
     6301 gagagataaa aaactgctct ttcaatatca gcacaagcat aagagataag gtgcagaaag
     6361 aatatgcatt cttttataaa cttgatatag taccaataga taataccagc tataggttga
     6421 taagttgtaa cacctcagtc attacacagg cctgtccaaa ggtatccttt gagccaattc
     6481 ccatacatta ttgtgccccg gctggttttg cgattctaaa atgtaataat aagacgttca
     6541 atggaacagg accatgtaca aatgtcagca cagtacaatg tacacatgga atcaggccag
     6601 tagtatcaac tcaactgctg ttaaatggca gtctagcaga agaagatgta gtaattagat
     6661 ctgccaattt cacagacaat gctaaaacca taatagtaca gctgaacaca tctgtagaaa
     6721 ttaattgtac aagacccaac aacaatacaa gaaaaagtat ccgtatccag aggggaccag
     6781 ggagagcatt tgttacaata ggaaaaatag gaaatatgag acaagcacat tgtaacatta
     6841 gtagagcaaa atggaatgcc actttaaaac agatagctag caaattaaga gaacaatttg
     6901 gaaataataa aacaataatc tttaagcaat cctcaggagg ggacccagaa attgtaacgc
     6961 acagttttaa ttgtggaggg gaatttttct actgtaattc aacacaactg tttaatagta
     7021 cttggtttaa tagtacttgg agtactgaag ggtcaaataa cactgaagga agtgacacaa
     7081 tcacactccc atgcagaata aaacaattta taaacatgtg gcaggaagta ggaaaagcaa
     7141 tgtatgcccc tcccatcagt ggacaaatta gatgttcatc aaatattact gggctgctat
     7201 taacaagaga tggtggtaat aacaacaatg ggtccgagat cttcagacct ggaggaggcg
     7261 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     7321 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     7381 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     7441 caatgacgct gacggtacag gccagacaat tattgtctga tatagtgcag cagcagaaca
     7501 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     7561 aacagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     7621 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     7681 ggagtaataa atctctggaa cagatttgga ataacatgac ctggatggag tgggacagag
     7741 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     7801 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     7861 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     7921 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     7981 cattatcgtt tcagacccac ctcccaatcc cgaggggacc cgacaggccc gaaggaatag
     8041 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatccttag
     8101 cacttatctg ggacgatctg cggagcctgt gcctcttcag ctaccaccgc ttgagagact
     8161 tactcttgat tgtaacgagg attgtggaac ttctgggacg cagggggtgg gaagccctca
     8221 aatattggtg gaatctccta caatattgga gtcaggagct aaagaatagt gctgttagct
     8281 tgctcaatgc cacagccata gcagtagctg aggggacaga tagggttata gaagtagtac
     8341 aaggagcttg tagagctatt cgccacatac ctagaagaat aagacagggc ttggaaagga
     8401 ttttgctata agatgggtgg cgcggccgct ctggtgagca agggcgagga gctgttcacc
     8461 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     8521 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     8581 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     8641 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     8701 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     8761 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     8821 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     8881 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     8941 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     9001 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     9061 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     9121 actctcggca tggacgagct gtacaagtaa ctcgagacct agaaaaacat ggagcaatca
     9181 caagtagcaa tacagcagct aacaatgctg cttgtgcctg gctagaagca caagaggagg
     9241 aagaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     9301 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     9361 aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     9421 ggcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     9481 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     9541 tgttacaccc tgtgagcctg catggaatgg atgaccctga gagagaagtg ttagagtgga
     9601 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     9661 agaactgctg acatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     9721 gtggcctggg cgggactggg gagtggcgag ccctcagatg ctgcatataa gcagctgctt
     9781 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     9841 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     9901 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     9961 aaaatctcta gcacccagga ggtagaggtt gcagtgagcc aagatcgcgc cactgcattc
    10021 cagcctgggc aagaaaacaa gactgtctaa aataataata ataagttaag ggtattaaat
    10081 atatttatac atggaggtca taaaaatata tatatttggg ctgggcgcag tggctcacac
    10141 ctgcgcccgg ccctttggga ggccgaggca ggtggatcac ctgagtttgg gagttccaga
    10201 ccagcctgac caacatggag aaaccccttc tctgtgtatt tttagtagat tttattttat
    10261 gtgtatttta ttcacaggta tttctggaaa actgaaactg tttttcctct actctgatac
    10321 cacaagaatc atcagcacag aggaagactt ctgtgatcaa atgtggtggg agagggaggt
    10381 tttcaccagc acatgagcag tcagttctgc cgcagactcg gcgggtgtcc ttcggttcag
    10441 ttccaacacc gcctgcctgg agagaggtca gaccacaggg tgagggctca gtccccaaga
    10501 cataaacacc caagacataa acacccaaca ggtccacccc gcctgctgcc caggcagagc
    10561 cgattcacca agacgggaat taggatagag aaagagtaag tcacacagag ccggctgtgc
    10621 gggagaacgg agttctatta tgactcaaat cagtctcccc aagcattcgg ggatcagagt
    10681 ttttaaggat aacttagtgt gtagggggcc agtgagttgg agatgaaagc gtagggagtc
    10741 gaaggtgtcc ttttgcgccg agtcagttcc tgggtggggg ccacaagatc ggatgagcca
    10801 gtttatcaat ccgggggtgc cagctgatcc atggagtgca gggtctgcaa aatatctcaa
    10861 gcactgattg atcttaggtt ttacaatagt gatgttaccc caggaacaat ttggggaagg
    10921 tcagaatctt gtagcctgta gctgcatgac tcctaaacca taatttcttt tttgtttttt
    10981 tttttttatt tttgagacag ggtctcactc tgtcacctag gctggagtgc agtggtgcaa
    11041 tcacagctca ctgcagcctc aacgtcgtaa gctcaagcga tcctcccacc tcagcctgcc
    11101 tggtagctga gactacaagc gacgccccag ttaatttttg tatttttggt agaggcagcg
    11161 ttttgccgtg tggccctggc tggtctcgaa ctcctgggct caagtgatcc agcctcagcc
    11221 tcccaaagtg ctgggacaac cggggccagt cactgcacct ggccctaaac cataatttct
    11281 aatcttttgg ctaatttgtt agtcctacaa aggcagtcta gtccccaggc aaaaaggggg
    11341 tttgtttcgg gaaagggctg ttactgtctt tgtttcaaac tataaactaa gttcctccta
    11401 aacttagttc ggcctacacc caggaatgaa caaggagagc ttggaggtta gaagcacgat
    11461 ggaattggtt aggtcagatc tctttcactg tctgagttat aattttgcaa tggtggttca
    11521 aagactgccc gcttctgaca ccagtcgctg cattaatgaa tcggccaacg cgcggggaga
    11581 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    11641 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    11701 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    11761 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    11821 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    11881 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    11941 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    12001 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    12061 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    12121 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    12181 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
    12241 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    12301 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    12361 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    12421 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    12481 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    12541 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    12601 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    12661 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    12721 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    12781 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    12841 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    12901 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    12961 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    13021 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    13081 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    13141 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    13201 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    13261 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    13321 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    13381 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    13441 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    13501 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
    13561 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
    13621 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
    13681 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
    13741 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
    13801 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg
    13861 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca ggggaggcag
    13921 agattgcagt aagctgagat cgcagcactg cactccagcc tgggcgacag agtaagactc
    13981 tgtctcaaaa ataaaataaa taaatcaatc agatattcca atcttttcct ttatttattt
    14041 atttattttc tattttggaa acacagtcct tccttattcc agaattacac atatattcta
    14101 tttttcttta tatgctccag ttttttttag accttcacct gaaatgtgtg tatacaaaat
    14161 ctaggccagt ccagcagagc ctaaaggtaa aaaataaaat aataaaaaat aaataaaatc
    14221 tagctcactc cttcacatca aaatggagat acagctgtta gcattaaata ccaaataacc
    14281 catcttgtcc tcaataattt taagcgcctc tctccaccac atctaactcc tgtcaaaggc
    14341 atgtgcccct tccgggcgct ctgctgtgct gccaaccaac tggcatgtgg actctgcagg
    14401 gtccctaact gccaagcccc acagtgtgcc ctgaggctgc cccttccttc tagcggctgc
    14461 ccccactcgg ctttgctttc cctagtttca gttacttgcg ttcagccaag gtctgaaact
    14521 aggtgcgcac agagcggtaa gactgcgaga gaaagagacc agctttacag ggggtttatc
    14581 acagtgcacc ctgacagtcg tcagcctcac agggggttta tcacattgca ccctgacagt
    14641 cgtcagcctc acagggggtt tatcacagtg cacccttaca atcattccat ttgattcaca
    14701 atttttttag tctctactgt gcctaacttg taagttaaat ttgatcagag gtgtgttccc
    14761 agaggggaaa acagtatata cagggttcag tactatcgca tttcaggcct ccacctgggt
    14821 cttggaatgt gtcccccgag gggtgatgac tacctcagtt ggatctccac aggtcacagt
    14881 gacacaagat aaccaagaca cctcccaagg ctaccacaat gggccgccct ccacgtgcac
    14941 atggccggag gaactgccat gtcggaggtg caagcacacc tgcgcatcag agtccttggt
    15001 gtggagggag ggaccagcgc agcttccagc catccacctg atgaacagaa cctagggaaa
    15061 gccccagttc tacttacacc aggaaaggct ggaagggcta atttggtccc aaaaaagaca
    15121 agagatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta
    15181 cacaccaggg ccagggatca gatatccact gacctttgga tggtgcttca agttagtacc
    15241 agttgaacca gagcaagtag aagaggccaa tgaaggagag aacaacagct tgttacaccc
    15301 tatgagccag catgggatgg aggacccgga gggagaagta ttagtgtgga agtttgacag
    15361 cctcctagca tttcgtcaca tggcccgaga gctgcatccg gagtactaca aagactgctg
    15421 acatcgagct ttctacaagg gactttccgc tggggacttt ccagg
//
