LOCUS       sequence-194068-        9483 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid containing the parB-m(sf)gfp fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9483)
  AUTHORS   van Raaphorst R, Kjos M, Veening JW
  TITLE     Chromosome segregation drives division site selection in 
            Streptococcus pneumoniae.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jul 3. pii: 201620608. doi: 
            10.1073/pnas.1620608114.
  PUBMED    28674002
REFERENCE   2  (bases 1 to 9483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Jul 3. pii: 201620608. doi: 
            10.1073/pnas.1620608114."
COMMENT     Sequence Label: pMK17-01
FEATURES             Location/Qualifiers
     source          1..9483
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      16..604
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     505..524
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     758..775
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    790..930
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(856..875)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1016..1038
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8277..8295)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8363..8467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8468..8536,8537..9328)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8686..8705)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
       61 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
      121 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
      181 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
      241 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
      301 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
      361 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
      421 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
      481 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
      541 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
      601 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
      661 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
      721 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      781 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
      841 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
      901 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
      961 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     1021 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca ggctaaacct
     1081 gctgctactg ctgcttggct tgccgcacta cctgtatttg gtaactgggc tttattagtt
     1141 tgatcagctt cacttgaatc aattggtttt gtatctgctt tttctgacac ttgtggtttt
     1201 ttagcttctt gagctactgg tttggttcca accaagacga tgcggtctgt cggaacttct
     1261 accacttcac ggagtttttc ttccttactt ccatcaggat taatcactgt aaagatacgt
     1321 tcttttccaa cttttccttc ttgttctaca cgagtttcac ctagatacag tgttgaatct
     1381 tttttctcaa ctgtcttgta ggccaaatct ttttcaacaa attcgagttt tggaagatct
     1441 tcttgtacag cagaaactgg tttttcctta gtcaactgga tacggtattc cttgacttgt
     1501 tttccacttt ctgaaacgag gcgaacaagt actggaaggc tatcttctcc actatctacc
     1561 acagttgaag ctacttgatt gttttcttca actgagactt ttggccgttg acctttatag
     1621 gtaatttgat agtcttgacg attttcagcg aaatcagcaa gttcttttcc atctacaaga
     1681 atctttgatt gagtgctttc ttgaggcaat tcacttggtg caaggaaggt catctcaatc
     1741 atcgcaacac cgctcttgtc agctttacgc tccatacgcc atctcatgac tttggctttg
     1801 acagctttaa atgttacgtt gatttcatca ccagaaatag tacataatgg atttccttac
     1861 gcgaaatacg ggcagacatg gcctgcccgg ttattattat ttttgacacc agaccaactg
     1921 gtaatggtag cgaccggcgc tcagcgatag cgctatcaaa agaatcttgc ttggcaaggt
     1981 tctttttttt gtcttttcaa aaaagcacga aaaaagcacc cataaggtaa acttttgagt
     2041 gctttgctat aaatacgctt cacagtttct tcttcatttt taacaaacaa aaaacccagc
     2101 tcattgagct gggtttaagc tggcttggcg gcgtcctagt actgcagaat tgccgacctt
     2161 gactagctta cttataaagc tcatccatgc cgtgagtgat accagcagct gtcacaaatt
     2221 caagcagcac catgtggtca cgcttttcat ttgggtcttt tgataatttg ctttgagttg
     2281 ataaataatg gttatcagga agaagaaccg ggccgtcacc gatcggtgta ttttgctgat
     2341 aatgatcagc aagctgaaca gaaccgtcct ctacgttatg acggattttg aagtttgctt
     2401 tgatgccgtt tttttgtttg tcagcagtga tgtatacgtt gtggctgttg aagttgtatt
     2461 caagtttgtg tccaaggatg tttccatctt ctttgaaatc aatgcctttc aattcaatgc
     2521 ggttcacaag tgtgtcacct tcaaacttca cttctgctct tgttttgtac gttccgtcat
     2581 ctttgaagct gatcgtacgc tcttgaacgt agccttcagg cattgcagat ttgaagaaat
     2641 catggcgttt catgtggtct ggataacgag agaagcattg aacgccgtaa gtcagcgtcg
     2701 ttacaagtgt cggccaagga accggcagct ttccagttgt gcaaataaac ttcaatgtca
     2761 gcttgccgtt tgttgcatct ccttcacctt caccgcgaac agagaatttg tgtccgttta
     2821 catctccgtc aagctcaacg agaatcggca caacacctgt gaacagctct tctccttttg
     2881 atccagcttt agctgcagct tctccaccag atcctctaga actagatttc atcaacaact
     2941 caatcaattt tttcttgatt tcttgttctt cttccgttcc ctcaaactct aatttttcta
     3001 attgagtttc aattttcttt aactgcgtga ttggcttttt agcggttttc ttttgggggc
     3061 taaatttagc cccctcttct tcaccacctt gttctttagc ttttctaatc agtttttgag
     3121 cggtataatg actgatacca aatctttcgg caattaagtc cctagtcttt cctttaacag
     3181 gttttccgtt gatttgtacc ccttgttttt tggctttatc taaaatatcc atatagtcat
     3241 caatgatggc tattttttct tcttctggca tttttaagag cgagcgagtt tgtaggttag
     3301 tttcatgcaa ctttagtctt gttacaagct cgtcctcgtc ttcatcaact ttcttagcga
     3361 gaacttcgta atcttcaggg agtctatttt cactaattag ttttttaata gcattaaatc
     3421 gtctatgccc tgcaatcaat tcatagctat atttatcacg ttgtttaaca agaacgggtt
     3481 gtaataagcc taactcgtct atgctatcag ctaacttatc aatgtcaact aaatcatagt
     3541 tgttattctc attttccttt atctcctcaa taggaatatt ttttgtattt gttttttttc
     3601 gcttgtgttc gtccttgttc ataaggtctg taaatccgaa actattactc atgtttgtcc
     3661 tccttattag ttaatcagta ctcgagatcc gaattctcat atttgcctcc ttaagatccg
     3721 cagactcaga atagactcat tctttgtctt tcccttacca accaccagac tcgttaacag
     3781 attgatagta atcgcaataa ttcctaacca gagaatcccc tcatcattga ctggttgcgg
     3841 atgcaaaatc ttcgtgacat tttccaaaat gactagaaca gagcccgtta cgagaatcac
     3901 agctgttact aaggctccta gcaggctaaa ccgcttatag cccaaggtgt actgattgtc
     3961 ttcttcacga ttggagattg tttctagaaa agctgatatt ccaattgcaa tcgcatctcc
     4021 caagtcatgc acagagtcag caagaacagc gctagaacca aatactccac ctgcaataaa
     4081 ctcaacaatg gcataagtca aatttaagaa aaaagccacc caaacagcat attttgcctt
     4141 catatttctc attcctttgt tataatagat ttatgaacac cttgttcatt atcattatcc
     4201 actaaaactc aaagaaagga tagaagcaaa acgtcagctt tattcagttc tgaacaattt
     4261 gccttaagtg tccatatgac taacattgac cgccgtatca gcagcatgcg gtaccgtcgg
     4321 ctgcatgcat cgatagatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt
     4381 ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc
     4441 gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagccaag taatcgtgaa
     4501 tgtcgctgtg aaatatttag atcaagaaac aaaggcaacc caaatctcgc aatttgagtt
     4561 gatattagaa aaacaagata attggaagat tgtaaaataa taaatattgg tacatgatta
     4621 cagatacttt gtaatcatgt actctttttg ataaaaaatt ggagattcct ttacaaatat
     4681 gctcttacgt gctataattt aagtatctat ttaaaaggag ttaataaata tgcggcaagg
     4741 tattcttaaa taaactgtca atttgatagc gggaacaaat aattggacgt ccttttttag
     4801 gagggcttag ttttttgtcc ccagtttaag aataccttta tcatgtgatt ctaaagtatc
     4861 cggagaatat ctgtatgctt tgtatgccta tggttatgca taaaaatccc agtgataaga
     4921 gtatttatca ctgggatttt tatgcccttt tgggcttttg aatggaggaa aatcacatga
     4981 aaattattaa tattggagtt ttagctcatg ttgatgcagg aaaaactacc ttaacagaaa
     5041 gcttattata taacagtgga gcgattacag aattaggaag cgtggacaaa ggtacaacga
     5101 ggacggataa tacgctttta gaacgtcaga gaggaattac aattcagaca ggaataacct
     5161 cttttcagtg ggaaaatacg aaggtgaaca tcatagacac gccaggacat atggatttct
     5221 tagcagaagt atatcgttca ttatcagttt tagatggggc aattctactg atttctgcaa
     5281 aagatggcgt acaagcacaa actcgtatat tatttcatgc acttaggaaa atggggattc
     5341 ccacaatctt ttttatcaat aagattgacc aaaatggaat tgatttatca acggtttatc
     5401 aggatattaa agagaaactt tctgccgaaa ttgtaatcaa acagaaggta gaactgtatc
     5461 ctaatgtgtg tgtgacgaac tttaccgaat ctgaacaatg ggatacggta atagagggaa
     5521 acgatgacct tttagagaaa tatatgtccg gtaaatcatt agaagcattg gaactcgaac
     5581 aagaggaaag cataagattt cagaattgtt ctctgttccc tctttatcat ggaagtgcaa
     5641 aaagtaatat agggattgat aaccttatag aagttattac taataaattt tattcatcaa
     5701 cacatcgagg tccgtctgaa ctttgcggaa atgttttcaa aattgaatat acaaaaaaaa
     5761 gacaacgtct tgcatatata cgcctttata gtggagtact acatttacga gattcggtta
     5821 gagtatcaga aaaagaaaaa ataaaagtta cagaaatgta tacttcaata aatggtgaat
     5881 tatgtaagat tgatcgagct tattctggag aaattgttat tttgcaaaat gagcttttga
     5941 agttaaatag tgttcttgga gatacaaaac tattgccaca gagaaaaaag attgaaaatc
     6001 cgcaccctct actacaaaca actgttgaac cgagtaaacc tgaacagaga gaaatgttgc
     6061 ttgatgccct tttggaaatc tcagatagtg atccgcttct acgatattac gtggattcta
     6121 cgacacatga aattatactt tctttcttag ggaaagtaca aatggaagtg attagtgcac
     6181 tgttgcaaga aaagtatcat gtggagatag aactaaaaga gcctacagtc atttatatgg
     6241 agagaccgtt aaaaaatgca gaatatacca ttcacatcga agtgccgcca aatcctttct
     6301 gggcttccat tggtttatct gtatcaccgc ttccgttggg aagtggaatg cagtatgaga
     6361 gctcggtttc tcttggatac ttaaatcaat catttcaaaa tgcagttatg gaagggatac
     6421 gctatggttg tgaacaagga ttgtatggtt ggaatgtgac ggactgtaaa atctgtttta
     6481 agtatggctt atactatagc cctgttagta ccccagcaga ttttcggatg cttgctccta
     6541 ttgtattgga gcaagccttt agaaaagctg gaacagaatt gttagagcca tatcttagtt
     6601 ttaaagttta tgcaccacag gaatatcttt cacgggcata taacgatgct cccaaatatt
     6661 gtgcaaatat cgtaaatact caactgaaaa ataatgaggt cattattatt ggagaaattc
     6721 ctgctcgatg tattcaagat tatcgcaatg atttaacttt ttttacaaat gggcttagtg
     6781 tttgtttagc agagctaaaa ggatatcagg ttaccactgg cgaacctgtt tgccagaccc
     6841 gtcgtctaaa tagtcagata gataaagtga gatatatgtt caataaaata acttagtgcg
     6901 ttttatgttg ttatataaat atggtttctt attaaataag atgaaatatt ctttaatata
     6961 gatttgaatt aaagtggaaa ggaggagatt gttattataa actacaagtg gatattgtgt
     7021 cctatttgtg gaaataaaac aagactacga atacgagtgg atactatact taaaaatttc
     7081 cctttatact gccccaaatg taagaacgaa actttaatta atgttcaaaa aatgaatata
     7141 ataacaatca aagagccaga cgccaagacg cagagccgat aatttgagaa atgaaactct
     7201 catcttatcg gctctttttg tttatctgaa ttttactgac tagccttcaa tatttcttgc
     7261 ttcattgata ccttttgcta gtgcttccat taaggatagt tctttgtctg taaagctagc
     7321 ggagcgcatt attttgcttt tgctgcgtac tcttcgttac gtttgatcat ttgttttctg
     7381 taccaagcaa agataccgat atagaataca aggaaggcta ctgcaccaag gattgctttg
     7441 atatcaccag ttgtagtgtt accaattgtc caaccaagaa gtttttcgat tggtccttca
     7501 agagtagagt gagtaatcaa ttgagtttgg ctcacacctt ctgggaaggc acctacacct
     7561 ttagcaagtt ctgttgcaaa tggtgcaata agtgtacctg aaagaaggaa gagtggcaac
     7621 aagagtgttc cgaagataat catacggagc aatttaccac gagttacaac caagagagct
     7681 ggagtaacac ccatagcgat gatacctgca agtggcaaga taccatttcc aacttttgaa
     7741 agaagcactg cttcaatcaa catgattggt gcaagtacgt tggcacaagc ccagatttca
     7801 gcacgaccag cgatgaatgg ccagtcaaga ccgatattga atttacgtcc ttgaagacgt
     7861 ttagtagcaa cgtttgtaat accttgtgat agtggttcta cggctgcgat gaaccatgaa
     7921 ccgataagtg agaagagttc caaagataca ccggcagtca aaccaagaga caaccatcct
     7981 ttgataacaa gacgccattt atctgcatct gcaacacctg caattggatg tggagttccc
     8041 ataataccga taacgatacc aaggatgaaa ccgatgaaga atttagatcc ccagaaaccg
     8101 attttcttgt tcaatttagc agcatcaaag tcatatttat caaggcctgg gaagaatttt
     8161 tcaaaaatct tatccaaaac catgataact gggttcatca tgtagttcat gtgagttgat
     8221 gtcattggtg atgaacttgg ggcgttaaga aggaattaat tcttgaagac gaaagggcct
     8281 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     8341 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     8401 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     8461 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     8521 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     8581 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     8641 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     8701 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     8761 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     8821 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     8881 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     8941 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     9001 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     9061 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     9121 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     9181 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     9241 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     9301 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     9361 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     9421 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     9481 aaa
//
