LOCUS       sequence-194076-        3641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S. meditearranea DAZAP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Iyer H, Issigonis M, Sharma PP, Extavour CG, Newmark PA
  TITLE     A premeiotic function for boule in the planarian Schmidtea 
            mediterranea.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):E3509-18. doi: 
            10.1073/pnas.1521341113. Epub 2016 Jun 2.
  PUBMED    27330085
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1521341113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-21- 21"; volume: "113"; issue: "25"; pages: 
            "E3509-18"
COMMENT     Sequence Label: DAZAP1
FEATURES             Location/Qualifiers
     source          1..3641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..48)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        84..102
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     84..101
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(520..538)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(542..560)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      574..621
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             1028..1822
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1082..1101)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1692..1711
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(1840..1908,1909..2700)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2058..2077)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2871..3459
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3360..3379
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3613..3630
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctaggc gcgccggatc
       61 ctaatacgac tcactatagg gagatttagg tgacactata gaagtgacct tgatggtaac
      121 gagattggaa aggttttcat cggaggttta tcacaagcta caaatgatga cagtcttcga
      181 aactatttcg atcagtttgg atctattgaa gattgtgttg tgatgatgga caataaaact
      241 ggtagatcca ggggattcgg ctatattaaa tttcacgaag aatcaacagt acgaacagtt
      301 cttgaagaag gacctcattt actagatgga aaaatagtag acgtcaagca atgcaatatt
      361 cacatgaagg gtagaaatcg aagaagcttg aaaatatttg ttggtggaat aggcctcgat
      421 gacaatgaag aaactattcg tcaattcttt tcgcaattcg gcaatgtgac agatgtaaac
      481 ttaatgctcg atcaaaccaa acaacgtcat aaaggtctcc ctttagtgag ggttaattct
      541 ccctatagtg agtcgtatta ggatccggcg cgcctagcat aaccccttgg ggcctctaaa
      601 cgggtcttga ggggtttttt gacgcgtcga tttcggccta ttggttaaaa aatgagctga
      661 tttaacaaaa atttaacgcg aattttaaca aaattcaggg cgcaagggct gctaaaggaa
      721 gcggaacacg tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta
      781 ctgggctatc tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg
      841 gcttacatgg cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc
      901 agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt
      961 gccgccaatg atctgatggc gcaggggatc aagatctgat caagagacag gatgaggatc
     1021 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     1081 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     1141 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     1201 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     1261 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     1321 ggggcaggat ctcctgtcat cccaccttgc tcctgccgag aaagtatcca tcatggctga
     1381 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     1441 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     1501 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     1561 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     1621 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     1681 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     1741 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     1801 ccttcttgac gagttcttct gaattgaaaa aggaagagta tgagtattca acatttccgt
     1861 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     1921 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     1981 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     2041 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     2101 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     2161 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     2221 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     2281 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     2341 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     2401 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     2461 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     2521 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     2581 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag ccaggcaact
     2641 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     2701 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     2761 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     2821 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2881 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2941 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3001 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3061 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3121 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3181 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3241 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3301 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3361 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3421 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3481 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3541 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3601 acgaccgagc gcagcgagtc agtgagcgag gaagcacgcg t
//
