LOCUS       sequence-194080-        6798 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Name: pBluescript-empty vector. pBluescript-LANApi,K14 (pDD2000) 
            bidirectional empty vector for backbone only..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6798)
  AUTHORS   Hilton IB, Dittmer DP
  TITLE     Quantitative analysis of the bidirectional viral G-protein-coupled 
            receptor and lytic latency-associated nuclear antigen promoter of 
            Kaposi's sarcoma-associated herpesvirus.
  JOURNAL   J Virol. 2012 Sep;86(18):9683-95. doi: 10.1128/JVI.00881-12. Epub 
            2012 Jun 27.
  PUBMED    22740392
REFERENCE   2  (bases 1 to 6798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.00881-12"; journalName: "J Virol"; date: "2012-09"; 
            volume: "86"; issue: "18"; pages: "9683-95"
COMMENT     Sequence Label: pDD2045
FEATURES             Location/Qualifiers
     source          1..6798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(35..52)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(206..794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(286..305)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(965..1756,1757..1825))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1588..1607
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1826..1930)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1957..2412
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2044..2063)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2254..2275
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2538..2560
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2552..2569
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2553..2569
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2576..2595
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2576..2594
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2627..2643
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2627..2643
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    2754..2875
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2918..4546)
                     /codon_start=1
                     /product="red-emitting variant of click beetle luciferase"
                     /label=CBRluc
                     /note="human codon-optimized"
                     /translation="MVKREKNVIYGPEPLHPLEDLTAGEMLFRALRKHSHLPQALVDVV
                     GDESLSYKEFFEATVLLAQSLHNCGYKMNDVVSICAENNTRFFIPVIAAWYIGMIVAPV
                     NESYIPDELCKVMGISKPQIVFTTKNILNKVLEVQSRTNFIKRIIILDTVENIHGCESL
                     PNFISRYSDGNIANFKPLHFDPVEQVAAILCSSGTTGLPKGVMQTHQNICVRLIHALDP
                     RYGTQLIPGVTVLVYLPFFHAFGFHITLGYFMVGLRVIMFRRFDQEAFLKAIQDYEVRS
                     VINVPSVILFLSKSPLVDKYDLSSLRELCCGAAPLAKEVAEVAAKRLNLPGIRCGFGLT
                     ESTSAIIQTLGDEFKSGSLGRVTPLMAAKIADRETGKALGPNQVGELCIKGPMVSKGYV
                     NNVEATKEAIDDDGWLHSGDFGYYDEDEHFYVVDRYKELIKYKGSQVAPAELEEILLKN
                     PCIRDVAVVGIPDLEAGELPSAFVVKQPGTEITAKEVYDYLAERVSHTKYLRGGVRFVD
                     SIPRNVTGKITRKELLKQLLVKAGG"
     RBS             3588..3596
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      4543..4552
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4605..4614
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4611..6239
                     /codon_start=1
                     /product="green-emitting variant of click beetle 
                     luciferase"
                     /label=CBG68luc
                     /note="human codon-optimized"
                     /translation="MVKREKNVIYGPEPLHPLEDLTAGEMLFRALRKHSHLPQALVDVV
                     GDESLSYKEFFEATVLLAQSLHNCGYKMNDVVSICAENNTRFFIPVIAAWYIGMIVAPV
                     NESYIPDELCKVMGISKPQIVFTTKNILNKVLEVQSRTNFIKRIIILDTVENIHGCESL
                     PNFISRYSDGNIANFKPLHFDPVEQVAAILCSSGTTGLPKGVMQTHQNICVRLIHALDP
                     RVGTQLIPGVTVLVYLPFFHAFGFSITLGYFMVGLRVIMFRRFDQEAFLKAIQDYEVRS
                     VINVPSVILFLSKSPLVDKYDLSSLRELCCGAAPLAKEVAEVAAKRLNLPGIRCGFGLT
                     ESTSANIHSLRDEFKSGSLGRVTPLMAAKIADRETGKALGPNQVGELCIKGPMVSKGYV
                     NNVEATKEAIDDDGWLHSGDFGYYDEDEHFYVVDRYKELIKYKGSQVAPAELEEILLKN
                     PCIRDVAVVGIPDLEAGELPSAFVVKQPGKEITAKEVYDYLAERVSHTKYLRGGVRFVD
                     SIPRNVTGKITRKELLKQLLEKAGG"
     RBS             6086..6094
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    6296..6417
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(6563..6581)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6602..6618)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6602..6618)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6615..6637)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6626..6642
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6650..6656,6657..6674,6675..6680))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6695..6716
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
       61 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      121 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      181 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      241 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      301 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      361 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      421 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      481 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      541 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      601 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      661 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      721 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      781 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      841 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      901 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      961 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1021 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1081 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1141 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1201 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1261 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1321 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1381 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1441 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1501 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1561 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1621 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1681 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1741 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1801 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1861 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1921 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa
     1981 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     2041 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     2101 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     2161 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     2221 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     2281 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     2341 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     2401 cgcttacaat ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     2461 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     2521 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata
     2581 cgactcacta tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgg
     2641 atcccccgat ccttatcgat tttaccacat ttgtagaggt tttacttgct ttaaaaaacc
     2701 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     2761 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     2821 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     2881 tctgctcgaa gcggccggcc gccccgactc tagattacta accgccggcc ttcaccaaca
     2941 attgtttcaa cagctccttg cgggtaattt tgcctgttac gttacgaggg atggagtcaa
     3001 caaaacgcac gccgccacgc aagtacttag tatggctcac acgttcagcc aggtaatcat
     3061 acacttcttt ggcggtaatt tctgtaccag gctgcttgac aacgaaagca gaaggcagtt
     3121 cgccggcctc cagatcagga atgccgacca cagcgacatc gcgaatgcat ggatttttca
     3181 acagaatctc ctccaactca gctggagcaa cctggctacc cttgtatttg atcagctcct
     3241 tgtaacgatc cacgacgtaa aaatgctcat cttcgtcgta atatccaaaa tcaccagaat
     3301 gcaaccagcc gtcgtcgtcg atggcctcct tggtagcttc aacgttattg acataaccct
     3361 tgctcaccat agggcctttg atacacagct cgcccacttg gttcgggccc aaagccttac
     3421 cagtttcgcg atcagcgatc ttagcagcca tgagtggagt gacacggccc aaagagccgc
     3481 tcttaaactc atccccgaga gtctggataa tcgcactggt agattcggtg aggccgaagc
     3541 cacaacgaat ccctggaaga ttcaagcgtt tggcggccac ttcagcgacc tccttagcca
     3601 gtggagcggc accgcaacac aattcacgca gtgaagacaa gtcgtacttg tccacgagtg
     3661 ggctcttaga caaaaacagg atcacgctag gcacgttgat gacactgcgg acttcataat
     3721 cttggatggc tttcaagaaa gcctcctgat caaaacggcg gaacataatc acgcggagac
     3781 cgaccataaa gtaacccaaa gtaatatgaa agccgaaagc atggaagaaa ggcaagtaga
     3841 ccaagacggt gacaccagga atcagctgag tgccgtagcg tggatcgaga gcatggatca
     3901 gacgcacgca aatgttttga tgggtctgca tgactccctt tgggagtcca gtagtaccgc
     3961 tgctacacag aatggctgca acttgttcca cagggtcgaa gtggagtggt ttaaagtttg
     4021 cgatgttgcc gtctgaatag cgagagatga aattaggcaa agattcgcaa ccgtgaatat
     4081 tctccacagt gtccaagatg atgatacgct taataaagtt ggtgcggctt tggacttcca
     4141 ggactttgtt cagaatattc ttagtggtga agacaatctg tggcttagag atacccatga
     4201 ctttacacag ttcgtcggga atgtagctct cgttgactgg agccacgatc ataccgatat
     4261 accatgcggc gatgactgga atgaagaaac gggtattgtt ttcagcacag atactaacga
     4321 cgtcgttcat cttgtagcca caattgtgga gggactgagc cagcaagacg gttgcctcaa
     4381 aaaactcctt gtagctcaaa gattcatcgc cgaccacatc gaccaaggct tgaggcaaat
     4441 gagagtgctt gcggagagca cgaaacagca tttcgccggc agtcaaatcc tccaaaggat
     4501 ggagaggctc agggccatag atgacatttt tctcacgctt taccatggtg gctttaccaa
     4561 cagtaccgga atgccaagct tggcattccg gtactgttgg taaagccacc atggtgaaac
     4621 gcgaaaagaa cgtgatctac ggcccagaac cactgcatcc actggaagac ctcaccgctg
     4681 gtgagatgct cttccgagca ctgcgtaaac atagtcacct ccctcaagca ctcgtggacg
     4741 tcgtgggaga cgagagcctc tcctacaaag aatttttcga agctactgtg ctgttggccc
     4801 aaagcctcca taattgtggg tacaaaatga acgatgtggt gagcatttgt gctgagaata
     4861 acactcgctt ctttattcct gtaatcgctg cttggtacat cggcatgatt gtcgcccctg
     4921 tgaatgaatc ttacatccca gatgagctgt gtaaggttat gggtattagc aaacctcaaa
     4981 tcgtctttac taccaaaaac atcttgaata aggtcttgga agtccagtct cgtactaact
     5041 tcatcaaacg catcattatt ctggataccg tcgaaaacat ccacggctgt gagagcctcc
     5101 ctaacttcat ctctcgttac agcgatggta atatcgctaa tttcaagccc ttgcattttg
     5161 atccagtcga gcaagtggcc gctattttgt gctcctccgg caccactggt ttgcctaaag
     5221 gtgtcatgca gactcaccag aatatctgtg tgcgtttgat ccacgctctc gaccctcgtg
     5281 tgggtactca attgatccct ggcgtgactg tgctggtgta tctgcctttc tttcacgcct
     5341 ttggtttctc tattaccctg ggctatttca tggtcggctt gcgtgtcatc atgtttcgtc
     5401 gcttcgacca agaagccttc ttgaaggcta ttcaagacta cgaggtgcgt tccgtgatca
     5461 acgtcccttc agtcattttg ttcctgagca aatctccttt ggttgacaag tatgatctga
     5521 gcagcttgcg tgagctgtgc tgtggcgctg ctcctttggc caaagaagtg gccgaggtcg
     5581 ctgctaagcg tctgaacctc cctggtatcc gctgcggttt tggtttgact gagagcactt
     5641 ctgctaacat ccatagcttg cgagacgagt ttaagtctgg tagcctgggt cgcgtgactc
     5701 ctcttatggc tgcaaagatc gccgaccgtg agaccggcaa agcactgggc ccaaatcaag
     5761 tcggtgaatt gtgtattaag ggccctatgg tctctaaagg ctacgtgaac aatgtggagg
     5821 ccactaaaga agccattgat gatgatggct ggctccatag cggcgacttc ggttactatg
     5881 atgaggacga acacttctat gtggtcgatc gctacaaaga attgattaag tacaaaggct
     5941 ctcaagtcgc accagccgaa ctggaagaaa ttttgctgaa gaacccttgt atccgcgacg
     6001 tggccgtcgt gggtatccca gacttggaag ctggcgagtt gcctagcgcc tttgtggtga
     6061 aacaacccgg caaggagatc actgctaagg aggtctacga ctatttggcc gagcgcgtgt
     6121 ctcacaccaa atatctgcgt ggcggcgtcc gcttcgtcga ttctattcca cgcaacgtta
     6181 ccggtaagat cactcgtaaa gagttgctga agcaactcct cgaaaaagct ggcggctagt
     6241 aaagtcttca tgattatcta gagtcggggc ggccggccgc ttcgagcaga catgataaga
     6301 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     6361 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     6421 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     6481 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtcgac ctcgaggggg
     6541 ggcccggtac cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat
     6601 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6661 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6721 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6781 tcggccaacg cgcgggga
//
