LOCUS       sequence-194089-         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vitro expression of YPet from the T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Stark JC, Huang A, Nguyen PQ, Dubner RS, Hsu KJ, Ferrante TC, 
            Anderson M, Kanapskyte A, Mucha Q, Packett JS, Patel P, Patel R, Qaq
            D, Zondor T, Burke J, Martinez T, Miller-Berry A, Puppala A, 
            Reichert K, Schmid M, Brand L, Hill LR, Chellaswamy JF, Faheem N, 
            Fetherling S, Gong E, Gonzalzles EM, Granito T, Koritsaris J, Nguyen
            B, Ottman S, Palffy C, Patel A, Skweres S, Slaton A, Woods T, 
            Donghia N, Pardee K, Collins JJ, Jewett MC
  TITLE     BioBits Bright: A fluorescent synthetic biology education kit.
  JOURNAL   Sci Adv. 2018 Aug 1;4(8):eaat5107. doi: 10.1126/sciadv.aat5107. 
            eCollection 2018 Aug.
  PUBMED    30083609
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-08-1"; volume: "4(8):eaat5107. doi"; pages: " 
            10.1126/sciadv.aat5107. eCollection 2018 Aug"
COMMENT     Sequence Label: pJL1-YPet
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..720)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELYK"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggtgagca aaggcgaaga gctgttcacc ggcgtggtgc ccatcctggt ggagctggac
       61 ggcgacgtga acggccacaa gttcagcgtg agcggcgagg gcgagggcga cgccacctac
      121 ggcaagctga ccctgaagct gctgtgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctggtgacca ccctgggcta cggcgtgcag tgcttcgccc ggtaccccga ccacatgaag
      241 cagcacgact tcttcaagag cgccatgccc gagggctacg tgcaggagcg gaccatcttc
      301 ttcaaggacg acggcaacta caagacccgg gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgga tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctgggccac
      421 aagctggagt acaactacaa cagccacaac gtgtacatca ccgccgacaa gcagaagaac
      481 ggcatcaagg ccaacttcaa gatccggcac aacatcgagg acggcggcgt gcagctggcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagct accagagcgc cctgttcaag gaccccaacg agaagcggga ccacatggtg
      661 ctgctggagt tcctgaccgc cgccggcatc accgagggca tgaacgagct ctataagtaa
//
