LOCUS       sequence-19409-e         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  TITLE     Bokoch Lab GTPases
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRK5-myc-Rac1-wt
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             43..72
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             79..657
                     /codon_start=1
                     /product="human Rac family small GTPase 1"
                     /label=Rac1
                     /translation="MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     regulatory      677..686
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(727..746)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tccaactgca cctcggttct atcgattgaa ttggccacca tggagcagaa gctgatctcc
       61 gaggaggacc tgggatccat gcaggccatc aagtgtgtgg tggtgggaga cggagctgta
      121 ggtaaaactt gcctactgat cagttacaca accaatgcat ttcctggaga atatatccct
      181 actgtctttg acaattattc tgccaatgtt atggtagatg gaaaaccggt gaatctgggc
      241 ttatgggata cagctggaca agaagattat gacagattac gccccctatc ctatccgcaa
      301 acagatgtgt tcttaatttg cttttccctt gtgagtcctg catcatttga aaatgtccgt
      361 gcaaagtggt atcctgaggt gcggcaccac tgtcccaaca ctcccatcat cctagtggga
      421 actaaacttg atcttaggga tgataaagac acgatcgaga aactgaagga gaagaagctg
      481 actcccatca cctatccgca gggtctagcc atggctaagg agattggtgc tgtaaaatac
      541 ctggagtgct cggcgctcac acagcgaggc ctcaagacag tgtttgacga agcgatccga
      601 gcagtcctct gcccgcctcc cgtgaagaag aggaagagaa aatgcctgct gttgtaagaa
      661 ttcctgcaga agcttggccg ccatggccca acttgtttat tgcagcttat aatggttaca
      721 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tt
//
