LOCUS       sequence-194091-        5104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Name: pCBG68-RBPjk-Delta-B. RBPjk binding site B mutation in 
            pCBG68-LANApi (pDD2002)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Hilton IB, Dittmer DP
  TITLE     Quantitative analysis of the bidirectional viral G-protein-coupled 
            receptor and lytic latency-associated nuclear antigen promoter of 
            Kaposi's sarcoma-associated herpesvirus.
  JOURNAL   J Virol. 2012 Sep;86(18):9683-95. doi: 10.1128/JVI.00881-12. Epub 
            2012 Jun 27.
  PUBMED    22740392
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.00881-12"; journalName: "J Virol"; date: "2012-09"; 
            volume: "86"; issue: "18"; pages: "9683-95"
COMMENT     Sequence Label: pDD2022
FEATURES             Location/Qualifiers
     source          1..5104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      376..385
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             382..2010
                     /codon_start=1
                     /product="green-emitting variant of click beetle 
                     luciferase"
                     /label=CBG68luc
                     /note="human codon-optimized"
                     /translation="MVKREKNVIYGPEPLHPLEDLTAGEMLFRALRKHSHLPQALVDVV
                     GDESLSYKEFFEATVLLAQSLHNCGYKMNDVVSICAENNTRFFIPVIAAWYIGMIVAPV
                     NESYIPDELCKVMGISKPQIVFTTKNILNKVLEVQSRTNFIKRIIILDTVENIHGCESL
                     PNFISRYSDGNIANFKPLHFDPVEQVAAILCSSGTTGLPKGVMQTHQNICVRLIHALDP
                     RVGTQLIPGVTVLVYLPFFHAFGFSITLGYFMVGLRVIMFRRFDQEAFLKAIQDYEVRS
                     VINVPSVILFLSKSPLVDKYDLSSLRELCCGAAPLAKEVAEVAAKRLNLPGIRCGFGLT
                     ESTSANIHSLRDEFKSGSLGRVTPLMAAKIADRETGKALGPNQVGELCIKGPMVSKGYV
                     NNVEATKEAIDDDGWLHSGDFGYYDEDEHFYVVDRYKELIKYKGSQVAPAELEEILLKN
                     PCIRDVAVVGIPDLEAGELPSAFVVKQPGKEITAKEVYDYLAERVSHTKYLRGGVRFVD
                     SIPRNVTGKITRKELLKQLLEKAGG"
     RBS             1857..1865
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    2067..2188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2078..2097)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2132..2151
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2436..2453)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2607..3195)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2687..2706)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3366..4157,4158..4226))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVAEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3989..4008
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4227..4331)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4358..4813
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4445..4464)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4655..4676
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    4944..4992
                     /note="synthetic polyadenylation signal"
     misc_feature    5006..5097
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5046..5065
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctgcaggaa ttcgataggc agctgcgcca
       61 cgaagcagtc acgtccccaa gagcagcagc ttggtccggc tgacttataa acagtgagta
      121 gcgcccgccc acttgtttcg ggcggcccgt aaggcaccct tatctttgaa atgctataag
      181 atgtggtaat catagtaata ccaggtgtac atgatttgtg ttaaggtgtg tggtggtttt
      241 cgaaaaacat gggttattgg ccgtttctgt ttctcacgcc cggattatat atctggactt
      301 gcctaggtag catccatggc aacgcaaggc cagcaaaccc actatcaagc ttggcattcc
      361 ggtactgttg gtaaagccac catggtgaaa cgcgaaaaga acgtgatcta cggcccagaa
      421 ccactgcatc cactggaaga cctcaccgct ggtgagatgc tcttccgagc actgcgtaaa
      481 catagtcacc tccctcaagc actcgtggac gtcgtgggag acgagagcct ctcctacaaa
      541 gaatttttcg aagctactgt gctgttggcc caaagcctcc ataattgtgg gtacaaaatg
      601 aacgatgtgg tgagcatttg tgctgagaat aacactcgct tctttattcc tgtaatcgct
      661 gcttggtaca tcggcatgat tgtcgcccct gtgaatgaat cttacatccc agatgagctg
      721 tgtaaggtta tgggtattag caaacctcaa atcgtcttta ctaccaaaaa catcttgaat
      781 aaggtcttgg aagtccagtc tcgtactaac ttcatcaaac gcatcattat tctggatacc
      841 gtcgaaaaca tccacggctg tgagagcctc cctaacttca tctctcgtta cagcgatggt
      901 aatatcgcta atttcaagcc cttgcatttt gatccagtcg agcaagtggc cgctattttg
      961 tgctcctccg gcaccactgg tttgcctaaa ggtgtcatgc agactcacca gaatatctgt
     1021 gtgcgtttga tccacgctct cgaccctcgt gtgggtactc aattgatccc tggcgtgact
     1081 gtgctggtgt atctgccttt ctttcacgcc tttggtttct ctattaccct gggctatttc
     1141 atggtcggct tgcgtgtcat catgtttcgt cgcttcgacc aagaagcctt cttgaaggct
     1201 attcaagact acgaggtgcg ttccgtgatc aacgtccctt cagtcatttt gttcctgagc
     1261 aaatctcctt tggttgacaa gtatgatctg agcagcttgc gtgagctgtg ctgtggcgct
     1321 gctcctttgg ccaaagaagt ggccgaggtc gctgctaagc gtctgaacct ccctggtatc
     1381 cgctgcggtt ttggtttgac tgagagcact tctgctaaca tccatagctt gcgagacgag
     1441 tttaagtctg gtagcctggg tcgcgtgact cctcttatgg ctgcaaagat cgccgaccgt
     1501 gagaccggca aagcactggg cccaaatcaa gtcggtgaat tgtgtattaa gggccctatg
     1561 gtctctaaag gctacgtgaa caatgtggag gccactaaag aagccattga tgatgatggc
     1621 tggctccata gcggcgactt cggttactat gatgaggacg aacacttcta tgtggtcgat
     1681 cgctacaaag aattgattaa gtacaaaggc tctcaagtcg caccagccga actggaagaa
     1741 attttgctga agaacccttg tatccgcgac gtggccgtcg tgggtatccc agacttggaa
     1801 gctggcgagt tgcctagcgc ctttgtggtg aaacaacccg gcaaggagat cactgctaag
     1861 gaggtctacg actatttggc cgagcgcgtg tctcacacca aatatctgcg tggcggcgtc
     1921 cgcttcgtcg attctattcc acgcaacgtt accggtaaga tcactcgtaa agagttgctg
     1981 aagcaactcc tcgaaaaagc tggcggctag taaagtcttc atgattatct agagtcgggg
     2041 cggccggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
     2101 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     2161 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     2221 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa
     2281 aatcgataag gatccgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc
     2341 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
     2401 tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     2461 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2521 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2581 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2641 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2701 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2761 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     2821 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2881 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2941 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3001 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     3061 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3121 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3181 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3241 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3301 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3361 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     3421 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     3481 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     3541 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     3601 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3661 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3721 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     3781 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     3841 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     3901 ttttctgcga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     3961 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     4021 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4081 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4141 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4201 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4261 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4321 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta
     4381 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4441 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4501 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4561 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4621 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4681 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     4741 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     4801 acgcttacaa tttgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     4861 cgggcctctt cgctattacg ccagcccaag ctaccatgat aagtaagtaa tattaaggta
     4921 cgggaggtac ttggagcggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg
     4981 gttttttgtg tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa
     5041 acaaactagc aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctctatc
     5101 gata
//
