LOCUS       sequence-194111-         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vitro expression of mKalama1 from the T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Stark JC, Huang A, Nguyen PQ, Dubner RS, Hsu KJ, Ferrante TC, 
            Anderson M, Kanapskyte A, Mucha Q, Packett JS, Patel P, Patel R, Qaq
            D, Zondor T, Burke J, Martinez T, Miller-Berry A, Puppala A, 
            Reichert K, Schmid M, Brand L, Hill LR, Chellaswamy JF, Faheem N, 
            Fetherling S, Gong E, Gonzalzles EM, Granito T, Koritsaris J, Nguyen
            B, Ottman S, Palffy C, Patel A, Skweres S, Slaton A, Woods T, 
            Donghia N, Pardee K, Collins JJ, Jewett MC
  TITLE     BioBits Bright: A fluorescent synthetic biology education kit.
  JOURNAL   Sci Adv. 2018 Aug 1;4(8):eaat5107. doi: 10.1126/sciadv.aat5107. 
            eCollection 2018 Aug.
  PUBMED    30083609
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-08-1"; volume: "4(8):eaat5107. doi"; pages: " 
            10.1126/sciadv.aat5107. eCollection 2018 Aug"
COMMENT     Sequence Label: pJL1-mKalama1
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..720)
                     /codon_start=1
                     /product="photostable monomeric blue variant of GFP (Ai et 
                     al., 2007)"
                     /label=mKalama1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVEMDGDVNGRKFSVRGVGEGDATHGKLTL
                     KFICTSGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GSYKTRAEVKFEGDTLVNRIVLKGTDFKEDGNILGHKLEYNMNVGNVYITADKQKNGIK
                     ANFEIRHNVEDGGVQLADHYQQNTPIGDGSVLLPDNHYLSVQVKLSKDPNEKRDHMVLL
                     EFRTAAGITPGMDELYK"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagatggac
       61 ggcgacgtaa acggccgcaa gttcagcgtg cgcggcgtgg gcgagggtga tgccacccac
      121 ggcaagctga ccctgaagtt catctgcacc tccggcaagc tgcccgtgcc ctggcctacc
      181 ctcgtgacca ccctgtctta cggcgtgcag tgcttctccc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcagcta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgtgctgaa gggcaccgac ttcaaggagg acggtaacat cctggggcac
      421 aagctggagt acaacatgaa cgtcgggaac gtctatatca cggccgacaa gcagaagaac
      481 ggcatcaaag cgaacttcga gatcaggcac aacgtcgagg acggcggcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggctccg tgctgctgcc cgacaaccac
      601 tacctgagcg tgcaggtgaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tccgcaccgc cgccgggatc actcccggca tggacgagct gtacaagtaa
//
