LOCUS       sequence-194115-       10044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  low-efficiency Cre used to turn on dio YFP/ PSD95-mCherry/ 
            Teal-gephyrin expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10044)
  AUTHORS   Villa KL, Berry KP, Subramanian J, Cha JW, Oh WC, Kwon HB, Kubota Y,
            So PT, Nedivi E
  TITLE     Inhibitory Synapses Are Repeatedly Assembled and Removed at 
            Persistent Sites In Vivo.
  JOURNAL   Neuron. 2016 Feb 3. pii: S0896-6273(16)00011-8. doi: 
            10.1016/j.neuron.2016.01.010.
  PUBMED    26853302
REFERENCE   2  (bases 1 to 10044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Feb 3. pii: S0896-6273(16)00011-8. doi: 
            10.1016/j.neuron.2016.01.010."
COMMENT     Sequence Label: pSin wPGK-Cre
FEATURES             Location/Qualifiers
     source          1..10044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(3..502)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(51..70)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     444..467
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(801..845)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1030..1263
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1756..1881
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1928..2561
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    2729..2863
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2753..2772)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2807..2826
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3288..3308)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          3438..3503
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(4637..4966)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4651..4786
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4951..4971
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(5041..5058)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5212..5800)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5292..5311)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5971..6762,6763..6831))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6594..6613
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6832..6936)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7004..7022
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7060..7082)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7182..7201
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7415..7434
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        7724..7742
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     7724..7741
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             7767..8000
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    8089..8677
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             8195..8206
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8604..8624
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     8690..8706
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     8691..8707
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(9764..9784)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    9853..9970
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtcgaaaggc ccggagatga ggaagaggag aacagcgcgg cagacgtgcg cttttgaagc
       61 gtgcagaatg ccgggcctcc ggaggacctt cgggcgcccg ccccgcccct gagcccgccc
      121 ctgagcccgc ccccggaccc accccttccc agcctctgag cccagaaagc gaaggagcaa
      181 agctgctatt ggccgctgcc ccaaaggcct acccgcttcc attgctcagc ggtgctgtcc
      241 atctgcacga gactagtgag acgtgctact tccatttgtc acgtcctgca cgacgcgagc
      301 tgcggggcgg gggggaactt cctgactagg ggaggagtag aaggtggcgc gaaggggcca
      361 ccaaagaacg gagccggttg gcgcctaccg gtggatgtgg aatgtgtgcg aggccagagg
      421 ccacttgtgt agcgccaagt gcccagcggg gctgctaaag cgcatgctcc agactgcctt
      481 gggaaaagcg cctcccctac ccggtagaat agatccgatg gtcgaggcta gtctcgtgat
      541 cgataccgtc gagatccgtt cactaatcga atggatctgt ctctgtctct ctctccacct
      601 tcttcttcta ttccttcggg cctgtcgggt cccctcgggg ttgggaggtg ggtctgaaac
      661 gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc aaaaactatt
      721 cttaaaccta ccaagcctcc tactatcatt atgaataatt ttatatacca cagccaattt
      781 gttatgttaa accaattcca caaacttgcc catttatcta attccaataa ttcttgttca
      841 ttcttttctt gctggttttg cgattcttca attaaggagt gtattaagct tgtgtaattg
      901 ttaatttctc tgtcccactc catccaggtc gtgtgattcc aaatctgttc cagagattta
      961 ttactccaac tagcattcca aggcacagca gtggtgcaaa tgagttttcc agagcaaccc
     1021 caaatcccca ggagctgttg atcctttagg tatctttcca cagccaggat tcttgcctgg
     1081 agctgcttga tgccccagac tgtgagttgc aacagatgct gttgcgcctc aatagccctc
     1141 agcaaattgt tctgctgctg cactatacca gacaataatt gtctggcctg taccgtcagc
     1201 gtcattgacg ctgcgcccat agtgcttcct gctgctccca agaacccaag gaacaaagct
     1261 cctattccca ctgctctttt ttctctctgc accactcttc tctttgcctt ggtgggtgct
     1321 actcctaatg gttcaatttt tactacttta tatttatata attcacttct ccaattgtcc
     1381 ctcatatctc ctcctccagg tctgaagatc agcggccgct tgctgtgcgg tggtcttact
     1441 tttgttttgc tcttcctcta tcttgtctaa agcttccttg gtgtctttta tctctatcct
     1501 ttgatgcaca caatagaggg ttgctactgt attatataat gatctaagtt cttctgatcc
     1561 tgtctgaagg gatggttgta gctgtcccag tatttgtcta cagccttctg atgtttctaa
     1621 caggccagga ttaactgcga atcgttctag ctccctgctt gcccatacta tatgttttaa
     1681 tttatatttt ttctttcccc ctggccttaa ccgaattttt tcccatcgcg atctaattct
     1741 cccccgctta atactgacgc tctcgcaccc atctctctcc ttctagcctc cgctagtcaa
     1801 aatttttggc gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag
     1861 ccgagtcctg cgtcgagaga gctcctctgg tttccctttc gctttcaagt ccctgttcgg
     1921 gcgccactgc tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca
     1981 gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc
     2041 ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag
     2101 agagacccag tacaggcaaa aagcagctgc ttatatgcag gatctgaggg ctcgccactc
     2161 cccagtcccg cccaggccac gcctccctgg aaagtcccca gcggaaagtc ccttgtagca
     2221 agctcgatat cagcagttct tgaagtactc cggatgcagc tctcgggcca cgtgatgaaa
     2281 tgctaggcgg ctgtcaaacc tccactctaa cacttctctc tccgggtcat ccatcccatg
     2341 caggctcaca gggtgtaaca agctggtgtt ctctccttta ttggcctctt ctaccttatc
     2401 tggctcaact ggtactagct tgtagcacca tccaaaggtc agtggatatc tgacccctgg
     2461 ccctggtgtg tagttctgct aatcagggaa gtagccttgt gtgtggtaga tccacagatc
     2521 aaggatatct tgtcttcttt gggagtgaat tagcccttcc aactactaag tttgtagtac
     2581 atatttaaca aatacaattt ctttaaaatg aaaataattc agaggaatca caggtttaga
     2641 gtaaatgaaa ccacaggtaa ttggcagtgg taatagggta tggggtggga agtttgggat
     2701 gattttggtt agcttgagtt atccagttga tccagacatg ataagataca ttgatgagtt
     2761 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     2821 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     2881 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     2941 ctacaaatgt ggtatggctg attatgatct ctagtcaagg cactatacat caaatattcc
     3001 ttattaaccc ctttacaaat taaaaagcta aaggtacaca atttttgagc atagttatta
     3061 atagcagaca ctctatgcct gtgtggagta agaaaaaaca gtatgttatg attataactg
     3121 ttatgcctac ttataaaggt tacagaatat ttttccataa ttttcttgta tagcagtgca
     3181 gctttttcct ttgtggtgta aatagcaaag caagcaagag ttctattact aaacacagca
     3241 tgactcaaaa aacttagcaa ttctgaagga aagtccttgg ggtcttctac ctttctcttc
     3301 ttttttggag gagtagaatg ttgagagtca gcagtagcct catcatcact agatggcatt
     3361 tcttctgagc aaaacaggtt ttcctcatta aaggcattcc accactgctc ccattcatca
     3421 gttccatagg ttggaatcta aaatacacaa acaattagaa tcagtagttt aacacattat
     3481 acacttaaaa attttatatt taccttagag ctttaaatct ctgtaggtag tttgtccaat
     3541 tatgtcacac cacagaagta aggttccttc acaaagatcc ggggcccact cataaatcca
     3601 gttgccgcca cggtagccaa tcaccgtatc gtataaatca tcgtcggtac gttcggcatc
     3661 gctcatcaca atacgtgcct ggacgtcgag gatttcgcgt gggtcaatgc cgcgccagat
     3721 ccacatcaga cggttaatca tgcgatacca gtgagggatg gttttaccat caagggccga
     3781 ctgcacaggc ggttgtgcgc cgtgattaaa gcggcggact agcgtcgagg tttcaggatg
     3841 tttaaagcgg ggtttgaaca gggtttcgct caggtttgcc tgtgtcatgg atgcagcctc
     3901 cagaatactt actggaaact attgtaaccc gcctgaagtt aaaaagaaca acgcccggca
     3961 gtgccaggcg ttgaaaagat tagcgaccgg agattggcgg gacgaatacg acgcccatat
     4021 cccacggctg ttcaatccag gtatcttgcg ggatatcaac aacatagtca tcaaccagcg
     4081 gacgaccagc cggttttgcg aagatggtga caaagtgcgc ttttggatac atttcacgaa
     4141 tcgcaaccgc agtaccaccg gtatccacca ggtcatcaat aacgatgaag ccttcgccat
     4201 cgccttctgc gcgtttcagc actttaagct cgcgctggtt gtcgtgatcg tagctggaaa
     4261 tacaaacggt atcgacatga cgaataccca gttcacgcgc cagtaacgca cccggtacag
     4321 accgccacgg cttacggcaa taatgccttt ccattgttca gaaggcatca gtcggcttgc
     4381 gagtttacgt gcatggatct gcaacatgtc ccaggtgacg atgtattttt cgctcatgtg
     4441 aagtgtccca gcctgtttat ctacggctta aaaagtgttc gaggggaaaa taggttgcgc
     4501 gagattatag agatctggcg cactaaaaac cagtatttca catgagtccg cgtcttttta
     4561 cgcactgcct ctccctgacg cgggataaag tggtattctc aaacatatct cgcaagcctg
     4621 tcttgtgtcc aagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct
     4681 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
     4741 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
     4801 gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
     4861 agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc
     4921 tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc cacagctgca
     4981 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     5041 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     5101 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5161 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     5221 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5281 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5341 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5401 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     5461 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5521 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5581 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     5641 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5701 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     5761 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     5821 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     5881 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     5941 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6001 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6061 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6121 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6181 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6241 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6301 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6361 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     6421 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     6481 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     6541 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     6601 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     6661 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     6721 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     6781 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     6841 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     6901 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     6961 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     7021 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     7081 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     7141 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     7201 actgagagtg caccattcga cgctctccct tatgcgactc ctgcattagg aagcagccca
     7261 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
     7321 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     7381 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     7441 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatctggc
     7501 tagcgatgac cctgctgatt ggttcgctga ccatttccgg gtgcgggacg gcgttaccag
     7561 aaactcagaa ggttcgtcca accaaaccga ctctgacggc agtttacgag agagatgata
     7621 gggtctgctt cagtaagcca gatgctacac aattaggctt gtacatattg tcgttagaac
     7681 gcggctacaa ttaatacata accttatgta tcatacacat acgatttagg tgacactata
     7741 gaatacacgg aattaattct agatgctgct agagattttc cacactgact aaaagggtct
     7801 gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca
     7861 aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc
     7921 tcagatctgg tctaaccaga gagacccagt acaagcaaaa agcagatctt gtcttcgttg
     7981 ggagtgaatt agcccttcca gtcccccctt ttcttttaaa aagtggctaa gatctacagc
     8041 tgccttgtaa gtcattggtc ttaaaggtac cgagctcgaa ttcccgatgc ggggaggcgg
     8101 cccaaaggga gatccgactc gtctgagggc gaaggcgaag acgcggaaga ggccgcagag
     8161 ccggcagcag gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg acgtagcaga
     8221 aggacgtccc gcgcagaatc caggtggcaa cacaggcgag cagccatgga aaggacgtca
     8281 gcttccccga caacaccacg gaattgtcag tgcccaacag ccgagcccct gtccagcagc
     8341 gggcaaggca ggcggcgatg agttccgccg tggcaatagg gagggggaaa gcgaaagtcc
     8401 cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca
     8461 cagtgcacac cacgccacgt tgcctgacaa cgggccacaa ctcctcataa agagacagca
     8521 accaggattt atacaaggag gagaaaatga aagccatacg ggaagcaata gcatgataca
     8581 aaggcattaa agcagcgtat ccacatagcg taaaaggagc aacatagtta agaataccag
     8641 tcaatctttc acaaattttg taatccagag gttgattatc ggaattccct cgaggtcgac
     8701 ggtatcgata agcttgatct agtggatccc cccgcgttaa tggctaatcg ccatcttcca
     8761 gcaggcgcac cattgcccct gtttcactat ccaggttacg gatatagttc atgacaatat
     8821 ttacattggt ccagccacca gcttgcatga tctccggtat tgaaactcca gcgcgggcca
     8881 tatctcgcgc ggctccgaca cgggcactgt gtccagacca ggccaggtat ctctgaccag
     8941 agtcatcctt agcgccgtaa atcaatcgat gagttgcttc aaaaatccct tccagggcgc
     9001 gagttgatag ctggctggtg gcagatggcg cggcaacacc attttttctg acccggcaaa
     9061 acaggtagtt attcggatca tcagctacac cagagacgga aatccatcgc tcgaccagtt
     9121 tagttacccc caggctaagt gccttctcta cacctgcggt gctaaccagc gttttcgttc
     9181 tgccaatatg gattaacatt ctcccaccgt cagtacgtga gatatcttta accctgatcc
     9241 tggcaatttc ggctatacgt aacagggtgt tataagcaat ccccagaaat gccagattac
     9301 gtatatcctg gcagcgatcg ctattttcca tgagtgaacg aacctggtcg aaatcagtgc
     9361 gttcgaacgc tagagcctgt tttgcacgtt caccggcatc aacgttttct tttcggatcc
     9421 gccgcataac cagtgaaaca gcattgctgt cacttggtcg tggcagcccg gaccgacgat
     9481 gaagcatgtt tagctggccc aaatgttgct ggatagtttt tactgccaga ccgcgcgcct
     9541 gaagatatag aagataatcg cgaacatctt caggttctgc gggaaaccat ttccggttat
     9601 tcaacttgca ccatgccgcc cacgaccggc aaacggacag aagcattttc caggtatgct
     9661 cagaaaacgc ctggcgatcc ctgaacatgt ccatcaggtt cttgcgaacc tcatcactcg
     9721 ttgcatcgac cggtaatgca ggcaaatttt ggtgtgacct gcataccttt ctcttcttct
     9781 taggtgtcat acctaagctg ggctgcagcc cgatccccaa agtggatctc tgctgtccct
     9841 gtaataaacc cgaaaatttt gaatttttgt aatttgtttt tgtaattctt tagtttgtat
     9901 gtctgttgct attatgtcta ctattctttc ccctgcactg taccccccaa tccccccttt
     9961 tcttttaaaa ttgtggatga atactgccat ttgtagatcc actagtaacg gccgccagtg
    10021 tgctggaatt cggcttgcaa gctt
//
