LOCUS       sequence-194129-        5128 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Name: pCBR-K14. K14 driving red luciferase reporter for CBR in pCBR 
            backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Hilton IB, Dittmer DP
  TITLE     Quantitative analysis of the bidirectional viral G-protein-coupled 
            receptor and lytic latency-associated nuclear antigen promoter of 
            Kaposi's sarcoma-associated herpesvirus.
  JOURNAL   J Virol. 2012 Sep;86(18):9683-95. doi: 10.1128/JVI.00881-12. Epub 
            2012 Jun 27.
  PUBMED    22740392
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.00881-12"; journalName: "J Virol"; date: "2012-09"; 
            volume: "86"; issue: "18"; pages: "9683-95"
COMMENT     Sequence Label: pDD2005
FEATURES             Location/Qualifiers
     source          1..5128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    46..167
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(57..76)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     111..130
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(415..432)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(586..1174)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(666..685)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1345..2136,2137..2205))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1968..1987
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2206..2310)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2337..2792
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2424..2443)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2634..2655
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    2923..2971
                     /note="synthetic polyadenylation signal"
     misc_feature    2985..3076
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3025..3044
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     primer_bind     3100..3116
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3101..3117
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      3497..3506
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3503..5128,1..3)
                     /codon_start=1
                     /product="red-emitting variant of click beetle luciferase"
                     /label=CBRluc
                     /note="human codon-optimized"
                     /translation="MVKREKNVIYGPEPLHPLEDLTAGEMLFRALRKHSHLPQALVDVV
                     GDESLSYKEFFEATVLLAQSLHNCGYKMNDVVSICAENNTRFFIPVIAAWYIGMIVAPV
                     NESYIPDELCKVMGISKPQIVFTTKNILNKVLEVQSRTNFIKRIIILDTVENIHGCESL
                     PNFISRYSDGNIANFKPLHFDPVEQVAAILCSSGTTGLPKGVMQTHQNICVRLIHALDP
                     RYGTQLIPGVTVLVYLPFFHAFGFHITLGYFMVGLRVIMFRRFDQEAFLKAIQDYEVRS
                     VINVPSVILFLSKSPLVDKYDLSSLRELCCGAAPLAKEVAEVAAKRLNLPGIRCGFGLT
                     ESTSAIIQTLGDEFKSGSLGRVTPLMAAKIADRETGKALGPNQVGELCIKGPMVSKGYV
                     NNVEATKEAIDDDGWLHSGDFGYYDEDEHFYVVDRYKELIKYKGSQVAPAELEEILLKN
                     PCIRDVAVVGIPDLEAGELPSAFVVKQPGTEITAKEVYDYLAERVSHTKYLRGGVRFVD
                     SIPRNVTGKITRKELLKQLLVKAGG"
     RBS             4453..4461
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tagtaatcta gagtcggggc ggccggccgc ttcgagcaga catgataaga tacattgatg
       61 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
      121 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
      181 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
      241 acctctacaa atgtggtaaa atcgataagg atccgtcgac cgatgccctt gagagccttc
      301 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
      361 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct
      421 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      481 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      541 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
      601 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      661 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      721 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
      781 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
      841 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
      901 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
      961 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1021 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1081 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1141 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1201 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     1261 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     1321 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     1381 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     1441 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     1501 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     1561 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     1621 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     1681 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     1741 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     1801 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     1861 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     1921 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     1981 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     2041 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     2101 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     2161 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     2221 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     2281 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc
     2341 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     2401 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     2461 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     2521 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     2581 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     2641 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     2701 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     2761 gaattttaac aaaatattaa cgcttacaat ttgccattcg ccattcaggc tgcgcaactg
     2821 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagcccaagc taccatgata
     2881 agtaagtaat attaaggtac gggaggtact tggagcggcc gcaataaaat atctttattt
     2941 tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag tactaacata cgctctccat
     3001 caaaacaaaa cgaaacaaaa caaactagca aaataggctg tccccagtgc aagtgcaggt
     3061 gccagaacat ttctctatcg ataggtaccg ggccccccct cgaggtcgac ggtatcgata
     3121 agcttgatag tgggtttgct ggccttgcgt tgccatggat gctacctagg caagtccaga
     3181 tatataatcc gggcgtgaga aacagaaacg gccaataacc catgtttttc gaaaaccacc
     3241 acacacctta acacaaatca tgtacacctg gtattactat ttcccacaca tcttatagca
     3301 tttcaaagat aagggtgcct tacgggccgc ccgaaacaag tgggcgggcg ctactcactg
     3361 tttataagtc agccggacca agctgctgct cttggggacg tgactgcttc gtggcgcagc
     3421 tgcctatcga attcctgcag cccgggctcg agatctgcga tctaagtaag cttggcattc
     3481 cggtactgtt ggtaaagcca ccatggtaaa gcgtgagaaa aatgtcatct atggccctga
     3541 gcctctccat cctttggagg atttgactgc cggcgaaatg ctgtttcgtg ctctccgcaa
     3601 gcactctcat ttgcctcaag ccttggtcga tgtggtcggc gatgaatctt tgagctacaa
     3661 ggagtttttt gaggcaaccg tcttgctggc tcagtccctc cacaattgtg gctacaagat
     3721 gaacgacgtc gttagtatct gtgctgaaaa caatacccgt ttcttcattc cagtcatcgc
     3781 cgcatggtat atcggtatga tcgtggctcc agtcaacgag agctacattc ccgacgaact
     3841 gtgtaaagtc atgggtatct ctaagccaca gattgtcttc accactaaga atattctgaa
     3901 caaagtcctg gaagtccaaa gccgcaccaa ctttattaag cgtatcatca tcttggacac
     3961 tgtggagaat attcacggtt gcgaatcttt gcctaatttc atctctcgct attcagacgg
     4021 caacatcgca aactttaaac cactccactt cgaccctgtg gaacaagttg cagccattct
     4081 gtgtagcagc ggtactactg gactcccaaa gggagtcatg cagacccatc aaaacatttg
     4141 cgtgcgtctg atccatgctc tcgatccacg ctacggcact cagctgattc ctggtgtcac
     4201 cgtcttggtc tacttgcctt tcttccatgc tttcggcttt catattactt tgggttactt
     4261 tatggtcggt ctccgcgtga ttatgttccg ccgttttgat caggaggctt tcttgaaagc
     4321 catccaagat tatgaagtcc gcagtgtcat caacgtgcct agcgtgatcc tgtttttgtc
     4381 taagagccca ctcgtggaca agtacgactt gtcttcactg cgtgaattgt gttgcggtgc
     4441 cgctccactg gctaaggagg tcgctgaagt ggccgccaaa cgcttgaatc ttccagggat
     4501 tcgttgtggc ttcggcctca ccgaatctac cagtgcgatt atccagactc tcggggatga
     4561 gtttaagagc ggctctttgg gccgtgtcac tccactcatg gctgctaaga tcgctgatcg
     4621 cgaaactggt aaggctttgg gcccgaacca agtgggcgag ctgtgtatca aaggccctat
     4681 ggtgagcaag ggttatgtca ataacgttga agctaccaag gaggccatcg acgacgacgg
     4741 ctggttgcat tctggtgatt ttggatatta cgacgaagat gagcattttt acgtcgtgga
     4801 tcgttacaag gagctgatca aatacaaggg tagccaggtt gctccagctg agttggagga
     4861 gattctgttg aaaaatccat gcattcgcga tgtcgctgtg gtcggcattc ctgatctgga
     4921 ggccggcgaa ctgccttctg ctttcgttgt caagcagcct ggtacagaaa ttaccgccaa
     4981 agaagtgtat gattacctgg ctgaacgtgt gagccatact aagtacttgc gtggcggcgt
     5041 gcgttttgtt gactccatcc ctcgtaacgt aacaggcaaa attacccgca aggagctgtt
     5101 gaaacaattg ttggtgaagg ccggcggt
//
