LOCUS       sequence-194150-        6231 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter for mouse Fgf21 promoter activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Miyake M, Nomura A, Ogura A, Takehana K, Kitahara Y, Takahara K, 
            Tsugawa K, Miyamoto C, Miura N, Sato R, Kurahashi K, Harding HP, 
            Oyadomari M, Ron D, Oyadomari S
  TITLE     Skeletal muscle-specific eukaryotic translation initiation factor 
            2alpha phosphorylation controls amino acid metabolism and fibroblast
            growth factor 21-mediated non-cell-autonomous energy metabolism.
  JOURNAL   FASEB J. 2016 Feb;30(2):798-812. doi: 10.1096/fj.15-275990. Epub 
            2015 Oct 20.
  PUBMED    26487695
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1096/fj.15-275990"; journalName: "FASEB J"; date: "2016-02"; 
            volume: "30"; issue: "2"; pages: "798-812"
COMMENT     Sequence Label: pGL3-mFgf21pro1-luc
FEATURES             Location/Qualifiers
     source          1..6231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1495..1504
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1501..3153
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1567..1585)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    3194..3315
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3205..3224)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3259..3278
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3563..3580)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3734..4322)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3814..3833)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4493..5284,5285..5353))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5116..5135
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5354..5458)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5485..5940
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5572..5591)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5782..5803
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    6071..6119
                     /note="synthetic polyadenylation signal"
     misc_feature    6133..6224
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     6173..6192
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccaa gatgagatca tttcttcaca ctgcagagtt
       61 ccagggccac atcagttcta aaggtccact ccccagcctc ctccccttag atgctccttg
      121 ggacttaggg gtcagtgctc cagtctcact cccaagagtc cttgctcagg gttcctccta
      181 gaaatccaaa ctcggcccca gcgcctttga ctgcaggaaa caacccagct cttccccttc
      241 accgagccta atcctcccac cccccaaagc atctggagag cacctgtgca ggacgctgtc
      301 tggtgaaaga agcactagga ttgcatcagg gaggacagca gctgagcaca aggcccgaat
      361 gctaagcagg ggttggtgag ggtgactggc ctgtggccag ctgggagact tccacctgct
      421 gggctgagga ctcctcttac acactgctga tacagctctc ctgatgaaag aaagcctcag
      481 acacgccccc cccccaaagc accttgaagc ttaaaattcc aaaaggatga accacaccag
      541 ctcagttgct tacacagaaa gtctgaagcc ccaggttcct tcttagtacc caagagccag
      601 accctagcct cctccctcag acccaagagc tagatcccag cctcctccct cagacccaag
      661 agccagaccc tagcctcctc cctcagatcc aggaatacag accccagcca cctccctcag
      721 acccagaact gcagagccca gccaccttca gacccaggag tgtagacttc agtctcctac
      781 ctcagaccca gaactgcaga ccccagctac cttcagaccc aggagtgtag accccagcct
      841 cctccttcag actcaggagt gtagacccca gcctcctccc tcagactcag gagtgcagac
      901 cctagcctcc tccctcagac tcaggagtgc agaccccagc ctcctccttc agactcagga
      961 gtgcagacaa gtccccttcc ttagacccaa ggccccaaac cccatctttc ctcccctcag
     1021 atactaaggt gaagatccca acctcctcca gagtccaacc ctcctccctc agaccaagga
     1081 gcacagaccc cagcctcctc ctccagattt aggagtgcag acccgccccc cagcactctt
     1141 cccccccaaa cccagggggc ccatgcctag cccttttcat tcagacccct gttggaaaga
     1201 cccccccatt gcatcatccg tccaggccgc cctggccacg gtggaattca ggttcctgcc
     1261 aagtgtgtca aatatcacgc gtcaggagtg gggagggcac gtgggcgggc ctgtctgggt
     1321 ataaattctg gtatttctgc gttcaccaga cagccttagt gtcttctcag ctggggattc
     1381 aacacaggag aaacagccat tcactttgcc tgagccccag tctgaacctg acccatccct
     1441 gctgggcaag atctgcgatc taagtaagct tggcattccg gtactgttgg taaagccacc
     1501 atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga
     1561 accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt
     1621 gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc
     1681 gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta
     1741 tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt
     1801 gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt
     1861 tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa
     1921 aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga
     1981 tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat
     2041 tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga
     2101 tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg
     2161 catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt
     2221 gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt
     2281 cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct tcaggattac
     2341 aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa aagcactctg
     2401 attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc tcccctctct
     2461 aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag gcaaggatat
     2521 gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc
     2581 gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa
     2641 acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat tatgtccggt
     2701 tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct
     2761 ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg cctgaagtct
     2821 ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat cttgctccaa
     2881 caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt
     2941 cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat
     3001 tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac
     3061 gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata
     3121 aaggccaaga agggcggaaa gatcgccgtg taattctaga gtcggggcgg ccggccgctt
     3181 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     3241 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     3301 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     3361 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat
     3421 ccgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
     3481 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
     3541 gccggcagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3601 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3661 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3721 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3781 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3841 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3901 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3961 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4021 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4081 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4141 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     4201 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4261 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4321 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4381 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4441 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4501 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4561 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4621 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4681 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4741 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4801 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4861 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     4921 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     4981 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5041 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5101 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5161 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5221 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5281 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5341 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5401 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5461 catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     5521 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     5581 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5641 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5701 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5761 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     5821 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5881 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     5941 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     6001 tattacgcca gcccaagcta ccatgataag taagtaatat taaggtacgg gaggtacttg
     6061 gagcggccgc aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga
     6121 atcgatagta ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa
     6181 ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt ctctatcgat a
//
