LOCUS       sequence-194152-        5020 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter for mouse Fgf21 promoter activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Miyake M, Nomura A, Ogura A, Takehana K, Kitahara Y, Takahara K, 
            Tsugawa K, Miyamoto C, Miura N, Sato R, Kurahashi K, Harding HP, 
            Oyadomari M, Ron D, Oyadomari S
  TITLE     Skeletal muscle-specific eukaryotic translation initiation factor 
            2alpha phosphorylation controls amino acid metabolism and fibroblast
            growth factor 21-mediated non-cell-autonomous energy metabolism.
  JOURNAL   FASEB J. 2016 Feb;30(2):798-812. doi: 10.1096/fj.15-275990. Epub 
            2015 Oct 20.
  PUBMED    26487695
REFERENCE   2  (bases 1 to 5020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1096/fj.15-275990"; journalName: "FASEB J"; date: "2016-02"; 
            volume: "30"; issue: "2"; pages: "798-812"
COMMENT     Sequence Label: pGL3-mFgf21pro3-luc
FEATURES             Location/Qualifiers
     source          1..5020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      284..293
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             290..1942
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(356..374)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    1983..2104
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1994..2013)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2048..2067
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2352..2369)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2523..3111)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2603..2622)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3282..4073,4074..4142))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3905..3924
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4143..4247)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4274..4729
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4361..4380)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4571..4592
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    4860..4908
                     /note="synthetic polyadenylation signal"
     misc_feature    4922..5013
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4962..4981
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcacgg tggaattcag gttcctgcca agtgtgtcaa
       61 atatcacgcg tcaggagtgg ggagggcacg tgggcgggcc tgtctgggta taaattctgg
      121 tatttctgcg ttcaccagac agccttagtg tcttctcagc tggggattca acacaggaga
      181 aacagccatt cactttgcct gagccccagt ctgaacctga cccatccctg ctgggcaaga
      241 tctgcgatct aagtaagctt ggcattccgg tactgttggt aaagccacca tggaagacgc
      301 caaaaacata aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga
      361 gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga
      421 tgcacatatc gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc
      481 agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa
      541 ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc
      601 cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac
      661 cgtggtgttc gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc
      721 aatcatccaa aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat
      781 gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga
      841 gtccttcgat agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct
      901 gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga
      961 tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca
     1021 tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt
     1081 aatgtataga tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag
     1141 tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata
     1201 cgatttatct aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg
     1261 ggaagcggtt gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga
     1321 gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa
     1381 agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt
     1441 taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa
     1501 tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc
     1561 ttactgggac gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta
     1621 caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat
     1681 cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt
     1741 tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag
     1801 tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa
     1861 aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa
     1921 gggcggaaag atcgccgtgt aattctagag tcggggcggc cggccgcttc gagcagacat
     1981 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     2041 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     2101 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     2161 tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc cgtcgaccga
     2221 tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg
     2281 tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc
     2341 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     2401 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     2461 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2521 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2581 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2641 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2701 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2761 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2821 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     2881 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     2941 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     3001 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     3061 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     3121 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     3181 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     3241 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     3301 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     3361 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     3421 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     3481 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     3541 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     3601 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     3661 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     3721 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     3781 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     3841 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     3901 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     3961 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     4021 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     4081 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     4141 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     4201 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     4261 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4321 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4381 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4441 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4501 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4561 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4621 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4681 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg ccattcgcca
     4741 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     4801 cccaagctac catgataagt aagtaatatt aaggtacggg aggtacttgg agcggccgca
     4861 ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac
     4921 taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc
     4981 ccagtgcaag tgcaggtgcc agaacatttc tctatcgata
//
